Allele Dmel\daw³

General Information
Symbol	Dmel\daw³	Species	D. melanogaster
Name		FlyBase ID	FBal0211071
Feature type	allele	Associated gene	Dmel\daw

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Parker et al., 2006)
Mutagen	ethyl methanesulfonate (Parker et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Contains a 2bp deletion at 1893-1894, which results in a frame shift in the prodomain. This is predicted to result in a truncated protein at L515. (Parker et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	hypoactive \| larval stage (Parker et al., 2006) lethal \| pupal stage (Parker et al., 2006) neuroanatomy defective \| embryonic stage (Parker et al., 2006, Pentek et al., 2009) neuroanatomy defective \| embryonic stage (with daw^Δ2) (Parker et al., 2006) uncoordinated \| adult stage (Parker et al., 2006)
Phenotype Manifest In
	abdominal segmental nerve (Parker et al., 2006) abdominal segmental nerve (with daw^Δ2) (Parker et al., 2006) intersegmental nerve (Parker et al., 2006) intersegmental nerve (with daw^Δ2) (Parker et al., 2006) intersegmental nerve branch ISNb of A1-7 (Pentek et al., 2009)
Detailed Description
	Statement Reference Homozygous embryos show stalling of ISNb axons at muscle 13 and incomplete extension with failure to form a synapse at muscle 12. (Pentek et al., 2009) Photoreceptor axon targeting defects are not seen in daw³/daw⁴ larvae. (Zhu et al., 2008) The majority (68%) of daw³ mutants die as white prepupae or as pharate adults and do not eclose. When reared at low density on nutritive agar plates, 7% eclose but most die shortly after eclosion. Rare escapers are fertile. The CNS of stage 16/17 daw³ embryos show occasional breaks and fusions of the longitudinal axon fascicles at the low incidence of 5%. The glial cell population shows no overt defects. Motorneuron pathfinding is disrupted in these embryos as although the ISNb and SNa axons exit the ventral nerve cord correctly and extend into their target field, they fail to advance far enough to innervate the appropriate muscle. The ISNb axons commonly stall at muscle 6 and fail to form synapses on muscles 12 and 13, or reach muscle 12 but are unable to form a synapse. The SNa usually extends into the target muscle but frequently exhibits the loss of one or both branches. In embryos from daw³/daw³ mothers mated to daw^δ2/daw^δ2 fathers, the incidence of ISNb and SNa defects is higher than in zygotic nulls. (Parker et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference daw³ has neuroanatomy defective \| embryonic stage phenotype, enhanceable by babo[+]/babo³² (Parker et al., 2006) daw³ has neuroanatomy defective \| embryonic stage phenotype, enhanceable by put⁸⁸/put[+] (Parker et al., 2006)
Enhancer of
Statement Reference daw[+]/daw³ is an enhancer of neuroanatomy defective \| embryonic stage phenotype of babo³² (Parker et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference daw³ has intersegmental nerve phenotype, enhanceable by babo[+]/babo³² (Parker et al., 2006) daw³ has intersegmental nerve phenotype, enhanceable by put⁸⁸/put[+] (Parker et al., 2006)
Enhancer of
Statement Reference daw[+]/daw³ is an enhancer of intersegmental nerve phenotype of babo³² (Parker et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference In daw³/+; babo³²/+; double mutant embryos, 20% of hemisegments show ISNb pathfinding defects as opposed to only 2% in daw³/+ single mutants and 4% in babo³²/+ single mutants. In daw³/+; put⁸⁸/+ double mutant embryos, 14% of hemisegments show ISNb pathfinding defects as opposed to only 2% in daw³/+ single mutants and no defects in put⁸⁸/+ single mutants. (Parker et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	daw^Δ1 (Parker et al., 2006)
Rescued by	daw³ is rescued by daw^+t7.8 (Parker et al., 2006) daw^Δ1/daw³ is rescued by daw^Scer\UAS.cPa/Scer\GAL4^Mef2.PR (Parker et al., 2006) daw^Δ1/daw³ is rescued by daw^Scer\UAS.cPa/Scer\GAL4^Rapgap1-OK6 (Parker et al., 2006) daw^Δ1/daw³ is rescued by daw^Scer\UAS.cPa/Scer\GAL4^repo (Parker et al., 2006)
Comments	Two copies of daw^Scer\UAS.cPa, under the control of Scer\GAL4^repo or Scer\GAL4^Mef2.PR, rescues the axon guidance defects of daw³/daw^δ1 embryos, while one copy provides partial rescue. Expression driven by Scer\GAL4^OK6 also reverses the defects. (Parker et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	daw³ (Parker et al., 2006, Zhu et al., 2008, Pentek et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Pentek et al., 2009, genesis 47(4): 261--273 Follistatin preferentially antagonizes activin rather than BMP signaling in Drosophila. [FBrf0207743] Zhu et al., 2008, Development 135(3): 513--521 Drosophila Activin-beta and the Activin-like product Dawdle function redundantly to regulate proliferation in the larval brain. [FBrf0202883] Parker et al., 2006, Development 133(24): 4981--4991 The divergent TGF-beta ligand Dawdle utilizes an activin pathway to influence axon guidance in Drosophila. [FBrf0194818]

Allele Dmel\daw3

Allele Dmel\daw³