|Feature type||allele||Associated gene||Dmel\daw|
|Allele class||loss of function allele|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Contains a 2bp deletion at 1893-1894, which results in a frame shift in the prodomain. This is predicted to result in a truncated protein at L515.
|Phenotype Manifest In|
Homozygous embryos show stalling of ISNb axons at muscle 13 and incomplete extension with failure to form a synapse at muscle 12.
The majority (68%) of daw3 mutants die as white prepupae or as pharate adults and do not eclose. When reared at low density on nutritive agar plates, 7% eclose but most die shortly after eclosion. Rare escapers are fertile. The CNS of stage 16/17 daw3 embryos show occasional breaks and fusions of the longitudinal axon fascicles at the low incidence of 5%. The glial cell population shows no overt defects. Motorneuron pathfinding is disrupted in these embryos as although the ISNb and SNa axons exit the ventral nerve cord correctly and extend into their target field, they fail to advance far enough to innervate the appropriate muscle. The ISNb axons commonly stall at muscle 6 and fail to form synapses on muscles 12 and 13, or reach muscle 12 but are unable to form a synapse. The SNa usually extends into the target muscle but frequently exhibits the loss of one or both branches. In embryos from daw3/daw3 mothers mated to dawδ2/dawδ2 fathers, the incidence of ISNb and SNa defects is higher than in zygotic nulls.
|Phenotype Manifest In|
In daw3/+; babo32/+; double mutant embryos, 20% of hemisegments show ISNb pathfinding defects as opposed to only 2% in daw3/+ single mutants and 4% in babo32/+ single mutants. In daw3/+; put88/+ double mutant embryos, 14% of hemisegments show ISNb pathfinding defects as opposed to only 2% in daw3/+ single mutants and no defects in put88/+ single mutants.
|Complementation & Rescue Data|
|Fails to complement|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 3 )|