Allele Dmel\flam^BG02658

General Information
Symbol	Dmel\flam^BG02658	Species	D. melanogaster
Name		FlyBase ID	FBal0212109
Feature type	allele	Associated gene	Dmel\flam

Map ( GBrowse )	Untitled Document
Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site X:21,502,538..21,502,538 (Mevel-Ninio et al., 2007, Brennecke et al., 2007)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{GT1}flam^BG02658 (Mevel-Ninio et al., 2007, Brennecke et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Mevel-Ninio et al., 2007) female sterile (with Df(1)flam^EY18P) (Mevel-Ninio et al., 2007) female sterile (with Df(1)l3) (Mevel-Ninio et al., 2007) female sterile (with Df(1)l15) (Mevel-Ninio et al., 2007) female sterile (with flam^EY2P) (Mevel-Ninio et al., 2007) female sterile (with flam^EY7P) (Mevel-Ninio et al., 2007) female sterile (with flam^EY13P) (Mevel-Ninio et al., 2007) female sterile (with flam^KG00476) (Mevel-Ninio et al., 2007) female sterile (with flam^py+P) (Mevel-Ninio et al., 2007) increased cell death (with flam^KG00476) (Zamparini et al., 2011)
Phenotype Manifest In
	dorsal appendage (Mevel-Ninio et al., 2007) egg (Mevel-Ninio et al., 2007) egg chamber (Mevel-Ninio et al., 2007) germline cell (Mevel-Ninio et al., 2007) ovariole (Mevel-Ninio et al., 2007) ovary (Mevel-Ninio et al., 2007) ovary (with flam^KG00476) (Zamparini et al., 2011)
Detailed Description
	Statement Reference flam[BG02658]/flam[KG00476] ovaries show defective germline and soma differentiation phenotypes. Somatic cells also die. (Zamparini et al., 2011) Homozygous females do not lay any eggs. flam^BG02658 flies have a permissive phenotype with respect to gypsy expression (assayed by expression of a P{gypsy-lacZ.p12} reporter construct in the ovaries). flam^BG02658 flies are unstable for ZAM expression (assayed by expression of a P{ZAM-lacZ.LTR} reporter construct in the ovaries). 24 hour old flam^BG02658 females have ovaries which are very small and contain rudimentary ovarioles within which no differentiation of germ cells has taken place. Ovarioles of 48 hour old mutant females are severely dystrophic, containing two to three completely disorganised egg chambers which contain a reduced number of abnormally shaped nuclei. Mature eggs are rarely formed, and those that do are smaller than normal and appear to lack dorsal-ventral and anterior-posterior polarities. The dorsal appendages are short and abnormally shaped. The follicular epithelium is completely disorganised in the ovaries of 6-day old mutant females. Mosaic females containing homozygous germline clones develop several completely normal ovarioles and are fertile. The eggs produced by these females give rise to normal adult flies. (Mevel-Ninio et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
NOT suppressed by
Statement Reference flam^BG02658 has female sterile phenotype, non-suppressible by DIP1^cos7a (Mevel-Ninio et al., 2007) flam^BG02658 has female sterile phenotype, non-suppressible by DIP1^t8 (Mevel-Ninio et al., 2007)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	flam^679-5b (Mevel-Ninio et al., 2007) flam^FM7c (Mevel-Ninio et al., 2007)
Fails to complement	flam^EY2P (Mevel-Ninio et al., 2007) flam^EY7P (Mevel-Ninio et al., 2007) flam^EY13P (Mevel-Ninio et al., 2007) flam^KG00476 (Mevel-Ninio et al., 2007) flam^py+P (Mevel-Ninio et al., 2007)
Comments
Stocks ( 1 )
Bloomington	13912 w¹¹¹⁸ P{GT1}flam^BG02658
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	flam^BG02658 flam^BG(P) (Mevel-Ninio et al., 2007) flam^BG (Zamparini et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Zamparini et al., 2011, Development 138(18): 4039--4050 Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. [FBrf0214783] Brennecke et al., 2007, Cell 128(6): 1089--1103 Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. [FBrf0200494] Mevel-Ninio et al., 2007, Genetics 175(4): 1615--1624 The flamenco locus controls the gypsy and ZAM retroviruses and is required for Drosophila oogenesis. [FBrf0200369]

Allele Dmel\flamBG02658

Allele Dmel\flam^BG02658