Allele Dmel\dl^Scer\UAS.cMa

General Information
Symbol	Dmel\dl^Scer\UAS.cMa	Species	D. melanogaster
Name		FlyBase ID	FBal0212140
Feature type	allele	Associated gene	Dmel\dl

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Matova and Anderson, 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the dl-A isoform. (Minidorff et al., 2007) Scer\UAS regulatory sequences are placed upstream of dl coding sequence. (Matova and Anderson, 2006)
Carried in construct	P{UASp-dl.M} (Matova and Anderson, 2006, Minidorff et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^hs.PB (Matova and Anderson, 2006) neuroanatomy defective, with Scer\GAL4^GMR.PF (Minidorff et al., 2007)
Phenotype Manifest In
	axon & eye photoreceptor cell, with Scer\GAL4^GMR.PF (Minidorff et al., 2007) lamina, with Scer\GAL4^GMR.PF (Minidorff et al., 2007) medulla, with Scer\GAL4^GMR.PF (Minidorff et al., 2007)
Detailed Description
	Statement Reference When dl[Scer\UAS.cMa] is expressed under the control of Scer\GAL4[GMR.PF], some photoreceptor R2-R5 axons project past the lamina and into the medulla. (Minidorff et al., 2007) Expression of dl^Scer\UAS.cMa, driven by Scer\GAL4^hs.PB at 37^oC (as a 1 hour pulse) is lethal. (Matova and Anderson, 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference dl^Scer\UAS.cMa, dl¹, Scer\GAL4^hs.PB is a suppressor of lethal \| larval stage phenotype of Dif¹ (Matova and Anderson, 2006) dl^Scer\UAS.cMa, Scer\GAL4^He.PZ, dl¹ is a suppressor of lethal \| larval stage phenotype of Dif¹ (Matova and Anderson, 2006) Scer\GAL4^e33C, dl^Scer\UAS.cMa, dl¹ is a suppressor of lethal \| larval stage phenotype of Dif¹ (Matova and Anderson, 2006)
Phenotype Manifest In
Suppressor of
Statement Reference dl^Scer\UAS.cMa, Scer\GAL4^He.PZ, dl¹ is a suppressor of hemocyte phenotype of Dif¹ (Matova and Anderson, 2006) dl^Scer\UAS.cMa, Scer\GAL4^He.PZ, dl¹ is a suppressor of hemolymph phenotype of Dif¹ (Matova and Anderson, 2006) dl^Scer\UAS.cMa, Scer\GAL4^srp.Hemo, dl¹ is a suppressor of hemocyte phenotype of Dif¹ (Matova and Anderson, 2006) dl^Scer\UAS.cMa, Scer\GAL4^srp.Hemo, dl¹ is a suppressor of hemolymph phenotype of Dif¹ (Matova and Anderson, 2006) Scer\GAL4^e33C, dl^Scer\UAS.cMa, dl¹ is a suppressor of hemocyte phenotype of Dif¹ (Matova and Anderson, 2006) Scer\GAL4^e33C, dl^Scer\UAS.cMa, dl¹ is a suppressor of hemolymph phenotype of Dif¹ (Matova and Anderson, 2006)
Additional Comments
Genetic Interactions
Statement Reference Ubiquitous expression of dl^Scer\UAS.cMa, driven by Scer\GAL4^hs.PB at 25^oC (without heat shock) fully rescues the lethality of Dif¹ dl¹ double mutants. Expression of dl^Scer\UAS.cMa in circulating haemocytes, lymph glands, and epidermis, but not in the fat body, under the control of Scer\GAL4^e33C, restores normal haemocyte numbers in Dif¹ dl¹ double mutants. The rescued blood cells are indistinguishable in morphology from wild-type uninfected haemocytes. In addition, the rescued animals have do not have any microbes in their haemolymph and 66% survive to adult stages. Expression of dl^Scer\UAS.cMa in circulating haemocytes, but not in other immune-responsive tissues, under the control of Scer\GAL4^He.PZ, results in an absence of microbes from the haemolymph, with approximately 45% of dl^Scer\UAS.cMa expressing Dif¹ dl¹ double mutants surviving till adulthood. Expression of dl^Scer\UAS.cMa in circulating haemocytes and lymph gland cells, under the control of Scer\GAL4^srp.Hemo, increases blood cell number to approximately 90% of wild-type in Dif¹ dl¹ double mutants and enables approximately 50% survival till adulthood. The majority of these larvae have no microbes in their haemolymph. (Matova and Anderson, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	dl^Scer\UAS.cMa
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Minidorff et al., 2007, Genetics 176(4): 2247--2263 A Gain-of-Function Screen for Genes That Influence Axon Guidance Identifies the NF-κB Protein Dorsal and Reveals a Requirement for the Kinase Pelle in Drosophila Photoreceptor Axon Targeting. [FBrf0202193] Matova and Anderson, 2006, Proc. Natl. Acad. Sci. U.S.A. 103(44): 16424--16429 Rel/NF-kappaB double mutants reveal that cellular immunity is central to Drosophila host defense. [FBrf0193396]

Allele Dmel\dlScer\UAS.cMa

Allele Dmel\dl^Scer\UAS.cMa