Allele Dmel\PGRP-LC^ΔE

General Information
Symbol	Dmel\PGRP-LC^ΔE	Species	D. melanogaster
Name		FlyBase ID	FBal0212184
Feature type	allele	Associated gene	Dmel\PGRP-LC
Also Known As	PGRP-LC^E12, PGRP-LC^DE12

Allele class
Mutagen	P-element activity, recombination
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Gottar et al., 2002) recombination (Gottar et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{XP}4396 (Gottar et al., 2002) P{XP}PGRP-LC⁰⁶⁹³ (Gottar et al., 2002)
Nature of the lesion	Statement Reference Recombination between P{XP}PGRP-LC⁰⁶⁹³ and P{XP}4396, resulting in an 8.7kb deletion which removes the entire PGRP-LC coding region. (Gottar et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective (Gottar et al., 2002, Bischoff et al., 2004, Gendrin et al., 2009, Maillet et al., 2008, Neyen et al., 2012) short lived \| conditional (Nam et al., 2012)
Phenotype Manifest In

Detailed Description
	Statement Reference PGRP-LC[ΔE] mutants show no effect on mortality within 24 hours of mild wounding in 7-8 days old adults. The mortality rate is increased to ~50% in response to more severe wounding. (Nam et al., 2012) Mutant flies infected with Erwinia carotovora carotovora by septic injury rapidly succumb to the infection. (Neyen et al., 2012) PGRP-LC[ΔE] mutant flies do not show a systemic response to genital infection with E.carotovora. (Gendrin et al., 2009) PGRP-LC[ΔE] mutants are susceptible to infection by Gram-negative bacteria. (Maillet et al., 2008) Hemolymph clots from PGRP-LC[ΔE] third instar larvae show normal melanization. (Bidla et al., 2007) Mutant flies show reduced survival compared to wild-type controls after infection with E. coli. (Bischoff et al., 2004) PGRP-LC^ΔE flies show a marked susceptibility to infection by Agrobacterium tumefaciens. (Gottar et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference PGRP-LC^ΔE is a suppressor of immune response defective phenotype of PGRP-SC1a^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 (Bischoff et al., 2006) PGRP-LC^ΔE is a suppressor of visible \| adult stage phenotype of PGRP-LF^{dsRNA.1.Scer\UAS}, Scer\GAL4^ptc-559.1 (Maillet et al., 2008)
Other
Statement Reference PGRP-LC^ΔE, PGRP-LE¹¹² has immune response defective phenotype (Aggarwal et al., 2008)
Phenotype Manifest In
Suppressor of
Statement Reference PGRP-LC^ΔE is a suppressor of wing phenotype of PGRP-LF^{dsRNA.1.Scer\UAS}, Scer\GAL4^ptc-559.1 (Maillet et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference PGRP-LE[112];PGRP-LC[ΔE] double mutant flies are rapidly killed by infection with the gram-negative pathogen Erwinia carotovora carotovora. (Aggarwal et al., 2008) A PGRP-LC[ΔE] background suppresses the wing notches seen when PGRP-LF[dsRNA.1.Scer\UAS] is expressed under the control of Scer\GAL4[ptc-559.1]. (Maillet et al., 2008) The increase in lethality seen in larvae expressing PGRP-SC1a[dsRNA.Scer\UAS] (under the control of Scer\GAL4[da.G32]) is completely suppressed in a PGRP-LC[ΔE] background. (Bischoff et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	PGRP-LC^ΔE/PGRP-LC^ΔE is rescued by PGRP-LC^+tNa (Neyen et al., 2012) PGRP-LC^ΔE/PGRP-LC^ΔE is rescued by PGRP-LC^LCx (Neyen et al., 2012) PGRP-LC^ΔE/PGRP-LC^ΔE is rescued by PGRP-LC^{T:Avic\GFP-EGFP} (Neyen et al., 2012) PGRP-LC^ΔE/PGRP-LC^ΔE is rescued by Scer\GAL4^yolk/PGRP-LC^{A.TM+IC.Scer\UAS} (Maillet et al., 2008)
Not rescued by	PGRP-LC^ΔE/PGRP-LC^ΔE is not rescued by PGRP-LC^LCa (Neyen et al., 2012) PGRP-LC^ΔE/PGRP-LC^ΔE is not rescued by PGRP-LC^LCa/PGRP-LC^LCy (Neyen et al., 2012) PGRP-LC^ΔE/PGRP-LC^ΔE is not rescued by PGRP-LC^LCy (Neyen et al., 2012) PGRP-LC^ΔE/PGRP-LC^ΔE is not rescued by Scer\GAL4^yolk/PGRP-LC^{A.EC.Scer\UAS} (Maillet et al., 2008) PGRP-LC^ΔE/PGRP-LC^ΔE is not rescued by Scer\GAL4^yolk/PGRP-LC^{A.TM+EC.Scer\UAS} (Maillet et al., 2008)
Comments	PGRP-LC[+tNa] (carried either in M{PGRP-LC.PGRP-LF} or in M{PGRP-LC}) rescues the susceptibility of PGRP-LC[ΔE] flies to infection with Erwinia carotovora carotovora by septic injury. PGRP-LC[T:Avic\GFP-EGFP] (carried in M{GFP-PGRP-LC.PGRP-LF}) rescues the susceptibility of PGRP-LC[ΔE] flies to infection with Erwinia carotovora carotovora by septic injury. PGRP-LC[LCa] and PGRP-LC[LCy] (either alone or in combination) fail to rescue the susceptibility of PGRP-LC[ΔE] flies to infection with Erwinia carotovora carotovora by septic injury. (Neyen et al., 2012) Expression of PGRP-LC[A.TM+IC.Scer\UAS] under the control of Scer\GAL4[yolk] rescues the susceptibility of PGRP-LC[ΔE] mutants to infection by Gram-negative bacteria. Expression of PGRP-LC[A.EC.Scer\UAS] under the control of Scer\GAL4[yolk] does not rescue the susceptibility of PGRP-LC[ΔE] mutants to infection by Gram-negative bacteria. Expression of PGRP-LC[A.TM+EC.Scer\UAS] under the control of Scer\GAL4[yolk] does not rescue the susceptibility of PGRP-LC[ΔE] mutants to infection by Gram-negative bacteria. (Maillet et al., 2008)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	PGRP-LC^DE12 (Akhouayri et al., 2011, Bosco-Drayon et al., 2012) PGRP-LC^DeltaE (Ferrandon, 2007.11.30) PGRP-LC^E12 (Gottar et al., 2006, Ferrandon, 2007.11.30, Zaidman-Remy et al., 2006, Gendrin et al., 2009, Neyen et al., 2012, Buchon et al., 2009, Ha et al., 2009, Bischoff et al., 2004, Bidla et al., 2007, Aymeric et al., 2010, Maillet et al., 2008, Nam et al., 2012) PGRP-LC^ΔE12 (Bosco-Drayon et al., 2012) PGRP-LC^ΔE (Davis et al., 2008, Kim et al., 2006, Aggarwal et al., 2008, Bischoff et al., 2004, Bischoff et al., 2006)
Name Synonym
Secondary FlyBase IDs
	FBal0135886 FBal0156116
References ( 26 )

Generate a list of	All references relating to this allele ( 26 )

List References by type	Research paper ( 22 ) Supplementary material ( 3 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 4 )
	Bosco-Drayon et al., 2012, Cell Host Microbe 12(2): 153--165 Peptidoglycan Sensing by the Receptor PGRP-LE in the Drosophila Gut Induces Immune Responses to Infectious Bacteria and Tolerance to Microbiota. [FBrf0219236] Nam et al., 2012, EMBO J. 31(5): 1253--1265 Genetic evidence of a redox-dependent systemic wound response via Hayan Protease-Phenoloxidase system in Drosophila. [FBrf0217593] Neyen et al., 2012, J. Immunol. 189(4): 1886--1897 Tissue- and Ligand-Specific Sensing of Gram-Negative Infection in Drosophila by PGRP-LC Isoforms and PGRP-LE. [FBrf0219094] Akhouayri et al., 2011, PLoS Pathog. 7(10): e1002319 Toll-8/tollo negatively regulates antimicrobial response in the Drosophila respiratory epithelium. [FBrf0216452] Show all research papers ...

Allele Dmel\PGRP-LCΔE

Allele Dmel\PGRP-LC^ΔE