FlyBase Allele Report: Dmel\Cat[UAS.cAa]

General Information

Symbol

Dmel\Cat^UAS.cAa

Species

D. melanogaster

Name

FlyBase ID

FBal0212304

Feature type

allele

Associated gene

Dmel\Cat

Associated Insertion(s)

Carried in Construct

P{UAS-Cat.A}

Also Known As

UAS-Cat, UAS-Catalase, UAS-Cat.A

Key Links

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Missirlis et al., 2001)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-Cat.A}

Cytology

Nature of the lesion

Statement

Reference

UASt sequences drive expression of Cat.

(Missirlis et al., 2001)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Cat

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by park²⁵

(Vincent et al., 2012)

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Arctic.UAS.Tag:SS(nec)}

(Rival et al., 2009)

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(nec)}

(Rival et al., 2009)

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

ameliorates Alexander disease

modeled by Hsap\GFAP^R79H.UAS

(Wang et al., 2011)

DOES NOT ameliorate cancer

modeled by dlg1^HMS01954

(Bunker et al., 2015)

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^P58S.cRa.UAS

(Chaplot et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Global overexpression of Cat ameliorates the synaptic overgrowth phenotype but not the locomotion defects of park²⁵/park²⁵ larvae modelling Parkinson's disease.

(Vincent et al., 2012)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult heart, with Scer\GAL4^sns.GCN

(Lim et al., 2014)

adult heart, with Scer\GAL4^Ugt36A1.PK

(Lim et al., 2014)

adult heart, with Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

(Lim et al., 2014)

adult heart | RU486 conditional, with Scer\GAL4^{Hand.ΔVM.Switch}

(Monnier et al., 2012)

lamellocyte | third instar larval stage | conditional, with Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Detailed Description

Statement

Reference

The size distribution of regenerated adult wings in flies expressing Cat^Scer\UAS.cAa under the control of Scer\GAL4^rn-GAL4-5 that were subjected to ablation of imaginal wing disc tissue during the larval stage, is comparable to that observed in similarly treated controls.

(Khan et al., 2017)

Expression of Cat^Scer\UAS.cAa specifically in nonmyocytic pericardial cells, under the control of Scer\GAL4^Dot.PK or Scer\GAL4^sns.GCN results in a significant reduction in the level of physiological reactive oxygen species. Compared to wild-type controls, these flies exhibit a marked deterioration in the regularity of heart rhythm, with an increased frequency of irregular beats, a broader distribution of heart periods, and significantly increased arrhythmia. These flies also exhibit a narrowed heart tube phenotype at 1 week of age, as manifested by significant decreases in the diastolic and systolic diameters, compared to control hearts. By 4 weeks of age, the flies with reduced reactive oxygen species levels exhibit enlarged heart tubes compared with control hearts because of a significantly greater diastolic diameters.

Expression of Cat^Scer\UAS.cAa in cardiomyocytes and nonmyocytic pericardial cells, under the control of Scer\GAL4^Hand.PA, which results in a significant reduction in the level of physiological reactive oxygen species, has no significant effects on any major aspects of heart function, including heart rhythm or heart tube dimensions.

Overexpression of Cat^Scer\UAS.cAa in nonmyocytic pericardial cells during embryogenesis and larval stages, under the control of Scer\GAL4^Dot.PK and Scer\GAL80^ts.αTub84B results in significant cardiac arrhythmias, compared to controls.

(Lim et al., 2014)

Animals expressing Cat^Scer\UAS.cAa via Scer\GAL4^da.PU are strikingly resistant to ethanol exposure compared to controls.

(Logan-Garbisch et al., 2014)

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^{Hand.ΔVM.Switch} (initiated by feeding flies RU486 during adulthood) improves cardiac performance. The heart period (mean time between successive end-diastolic positions) and frequency of arrhythmia are reduced in 45 day and 60 day old mutant flies compared to age-matched controls. Expression of Cat^Scer\UAS.cAa also prevents the age-related decrease in End-Diastolic Diameter (EDD) seen in wild type flies; EDD remains stable between 10 days and 60 days.

(Monnier et al., 2012)

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^Antp-10 reduces the increase in lamellocyte number seen following wasp infestation.

(Sinenko et al., 2011)

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^Act does not rescue the reduction in lifespan seen in flies under hyperoxia.

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^ple.PF does not rescue the degeneration of dopaminergic neurons that is seen in flies under hyperoxia.

(Gruenewald et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cat^UAS.cAa, Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B has abnormal oxidative stress response phenotype, suppressible by p38a¹³/p38b^156A

(Lim et al., 2014)

Cat^UAS.cAa has increased cell death phenotype, suppressible by Scer\GAL4^GMR.PF/PHGPx^UAS.cMa

(Yang et al., 2013)

Cat^UAS.cAa has chemical sensitive phenotype, suppressible by Scer\GAL4^GMR.PF/PHGPx^UAS.cMa

(Yang et al., 2013)

Suppressor of

Statement

Reference

Cat^UAS.cAa, Scer\GAL4^da.G32 is a suppressor of short lived phenotype of ACOX1^N250S.UAS, Scer\GAL4^da.G32

(Chung et al., 2020)

Cat^UAS.cAa, Scer\GAL4^egr-GAL4 is a suppressor of short lived phenotype of ACOX1^N250S.UAS, Scer\GAL4^egr-GAL4

(Chung et al., 2020)

Cat^UAS.cAa, Scer\GAL4^egr-GAL4 is a suppressor of abnormal locomotor behavior | adult stage phenotype of ACOX1^N250S.UAS, Scer\GAL4^egr-GAL4

(Chung et al., 2020)

Cat^UAS.cAa, Scer\GAL4^en.PU is a suppressor | partially of increased cell death | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

Cat^UAS.cAa, Scer\GAL4^en.PU is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

Cat^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, egr^UAS.cIa

(Yang et al., 2013)

Cat^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of PHGPx^UAS.cMa

(Yang et al., 2013)

Cat^UAS.cAa/Scer\GAL4^GMR.PF is a suppressor of chemical sensitive phenotype of PHGPx^UAS.cMa

(Yang et al., 2013)

Scer\GAL4^Act5C.PU/Cat^UAS.cAa is a suppressor of abnormal neuroanatomy | recessive | third instar larval stage phenotype of park²⁵

(Vincent et al., 2012)

Cat^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Cln3^UAS.cTa

(Tuxworth et al., 2011)

Cat^UAS.cAa, Scer\GAL4^repo is a suppressor of increased cell death | progressive phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Cat^UAS.cAa, Scer\GAL4^repo is a suppressor of paralytic phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Cat^UAS.cAa, Scer\GAL4^Hml.Δ is a suppressor | partially of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Cat^UAS.cAa, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(nec)}/Hsap\APP^{1-42.Scer\UAS.T:SS-nec}, Scer\GAL4^elav-C155

(Rival et al., 2009)

Cat^UAS.cAa, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155

(Rival et al., 2009)

Cat^UAS.cAa, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior phenotype of Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155

(Rival et al., 2009)

NOT Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cWa, Cat^UAS.cAa, Scer\GAL4^repo is a non-suppressor of abnormal neuroanatomy phenotype of Cpes^KO

(Kunduri et al., 2018)

Cat^UAS.cAa/Scer\GAL4^ninaE.PT is a non-suppressor of abnormal neurophysiology | somatic clone | adult stage phenotype of fh¹

(Chen et al., 2016)

Cat^UAS.cAa, Scer\GAL4^Mef2.PR is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Cat^UAS.cAa, Scer\GAL4^Mef2.PR is a non-suppressor of partially lethal - majority die phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Cat^UAS.cAa, Scer\GAL4^Bx-MS1096 is a non-suppressor of neoplasia | third instar larval stage phenotype of Scer\GAL4^Bx-MS1096, dlg1^HMS01954

(Bunker et al., 2015)

Cat^UAS.cAa, Scer\GAL4^hs.2sev is a non-suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Scer\GAL4^hs.2sev, Cat^UAS.cAa/PHGPx^UAS.cMa is a non-suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Scer\GAL4^Act5C.PU/Cat^UAS.cAa is a non-suppressor of abnormal locomotor behavior | recessive | larval stage phenotype of park²⁵

(Vincent et al., 2012)

Scer\GAL4^Act5C.PU/Cat^UAS.cAa is a non-suppressor of abnormal neurophysiology | larval stage phenotype of park^Z3678/park²⁵

(Vincent et al., 2012)

Cat^UAS.cAa/Scer\GAL4^Mef2.PR is a non-suppressor of partially lethal - majority die phenotype of p38a¹, p38b^Δ25

(Vrailas-Mortimer et al., 2011)

Cat^UAS.cAa/Cat^Scer\UAS.cAa, Scer\GAL4^da.G32 is a non-suppressor of lethal phenotype of Scer\GAL4^da.G32, fh^dsRNA.IR.UAS

(Anderson et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cat^UAS.cAa, Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B has adult heart phenotype, suppressible by p38a¹³/p38b^156A

(Lim et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^sv.PJ/Cat^UAS.cAa is a suppressor | partially of retina phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/Cat^UAS.cAa is a suppressor | partially of lipid droplet | increased number | adult stage phenotype of Tace¹

(Muliyil et al., 2020)

Cat^UAS.cAa, Scer\GAL4^en.PU is a suppressor | partially of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

Cat^UAS.cAa, Scer\GAL4^en.PU is a suppressor of wing disc | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

Cat^UAS.cAa, Scer\GAL4^en.PU is a suppressor of hemocyte | third instar larval stage phenotype of Scer\GAL4^en.PU, vtd^GL00522

(Liu et al., 2015)

Cat^UAS.cAa, Scer\GAL4^CCAP.PP is a suppressor of wing phenotype of Idh^{R195H.UAS.Tag:FLAG}, Scer\GAL4^CCAP.PP

(Reitman et al., 2015)

Cat^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, egr^UAS.cIa

(Yang et al., 2013)

Cat^UAS.cAa, Scer\GAL4^hs.2sev is a suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Scer\GAL4^hs.2sev, Cat^UAS.cAa/PHGPx^UAS.cMa is a suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Scer\GAL4^Act5C.PU/Cat^UAS.cAa is a suppressor of terminal bouton | third instar larval stage phenotype of park²⁵

(Vincent et al., 2012)

Cat^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Cln3^UAS.cTa

(Tuxworth et al., 2011)

Cat^UAS.cAa, Scer\GAL4^repo is a suppressor of adult brain phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Cat^UAS.cAa, Scer\GAL4^repo is a suppressor of neuron | cell non-autonomous phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Cat^UAS.cAa, Scer\GAL4^repo is a suppressor of lamina phenotype of Hsap\GFAP^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Cat^UAS.cAa, Scer\GAL4^Hml.Δ is a suppressor | partially of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

NOT Suppressor of

Statement

Reference

Hsap\SOD1^UAS.cWa, Cat^UAS.cAa, Scer\GAL4^repo is a non-suppressor of adult brain cell body glial cell phenotype of Cpes^KO

(Kunduri et al., 2018)

Hsap\SOD1^UAS.cWa, Cat^UAS.cAa, Scer\GAL4^repo is a non-suppressor of adult brain phenotype of Cpes^KO

(Kunduri et al., 2018)

Cat^UAS.cAa/Scer\GAL4^ninaE.PT is a non-suppressor of eye photoreceptor cell | somatic clone | adult stage phenotype of fh¹

(Chen et al., 2016)

Cat^UAS.cAa, Scer\GAL4^Mef2.PR is a non-suppressor of mitochondrion | adult stage phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Cat^UAS.cAa/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing disc | third instar larval stage phenotype of dlg1^HMS01954

(Bunker et al., 2015)

Cat^UAS.cAa, Scer\GAL4^{Hand.ΔVM.Switch} is a non-suppressor of adult heart | RU486 conditional phenotype of Scer\GAL4^{Hand.ΔVM.Switch}, fh^dsRNA.IR.UAS

(Tricoire et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^ninaE.PT fails to rescue the neurodegeneration and electrophysiology defect seen in fh¹ mutant photoreceptor clones.

(Chen et al., 2016)

The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology defects in adult indirect flight muscles (enlarged clustered mitochondria) characteristic for animals expressing clu^GD13926 under the control of Scer\GAL4^Mef2.PR cannot be suppressed by co-expression of Cat^Scer\UAS.cAa.

(Wang et al., 2016)

The co-expression of Cat^Scer\UAS.cAa does not suppress the tumor phenotype and tissue disorganization resulting from the expression of dlg1^HMS01954 under the control of Scer\GAL4^Bx-MS1096.

(Bunker et al., 2015)

The expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient vtd RNAi) leads to a significant increase in apoptosis in the posterior compartment of the third instar larval wing disc, which is suppressed by co-expressing Cat^Scer\UAS.cAa.

Expression of vtd^GL00522 under the control of Scer\GAL4^en.PU (together with Dicer-2, for efficient RNAi) results in increased numbers of hemocytes on the surface of third instar larval wing discs, as compared to controls, which are suppressed by co-expressing Cat^Scer\UAS.cAa.

(Liu et al., 2015)

A p38b^156A, p38a¹³ double mutant background partially restores a normal heart rhythm and normalizes the diastolic and systolic heart diameters of flies expressing Cat^Scer\UAS.cAa under the control of Scer\GAL4^Dot.PK.

(Lim et al., 2014)

Expression of Cat^Scer\UAS.cAa fails to suppress the heart defects seen when fh^{dsRNA.IR.Scer\UAS} is expressed under the control of Scer\GAL4^{Hand.ΔVM.Switch}.

(Tricoire et al., 2014)

Co-expression of Cat^Scer\UAS.cAa and PHGPx^Scer\UAS.cMa, under the control of Scer\GAL4^GMR.PF rescues the cell death found after H[[2]]O[[2]] application on larval eye discs.

(Yang et al., 2013)

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^Act5C.PU suppresses the synaptic overgrowth, but not the locomotion defects of park²⁵/park²⁵ larvae.

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^Act5C.PU does not rescue the muscle resting membrane potential of park²⁵/park^Z3678 larvae.

(Vincent et al., 2012)

Co-expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^GMR.PF almost completely suppresses the cln3^Scer\UAS.cTa-overexpression phenotype in the eye.

(Tuxworth et al., 2011)

Expression of Cat^Scer\UAS.cAa under the control of Scer\GAL4^Mef2.PR does not rescue the reduced viability seen in p38b^Δ25 Mpk2¹ double mutants.

(Vrailas-Mortimer et al., 2011)

Xenogenetic Interactions

Statement

Reference

Overexpression of Cat^Scer\UAS.cAa in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS necrotic neurons reduces the eye defect and JNK activation without affecting primary necrosis.

Overexpression of Cat^Scer\UAS.cAa and PHGPx^Scer\UAS.cMa in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS necrotic neurons reduces the eye defect and JNK activation without affecting primary necrosis.

(Yang et al., 2013)

Overexpression of Cat^Scer\UAS.cAa in flies expressing Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of Cat^Scer\UAS.cAa also decreases the number of seizures. Protection from cellular toxicity does not alter the number of inclusion bodies.

Overexpression of Cat^Scer\UAS.cAa in flies expressing Hsap\GFAP^{R79H.Scer\UAS} under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of Cat^Scer\UAS.cAa also decreases the number of seizures. Protection from cellular toxicity does not alter the number of inclusion bodies.

(Wang et al., 2011)

Expression of Cat^Scer\UAS.cAa moderately suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Overexpression of Cat^Scer\UAS.cAa suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Cat^Scer\UAS.cAa suppresses the decline in locomotor function of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Cat^Scer\UAS.cAa suppresses the decline in locomotor function of flies expressing Hsap\APP^{Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

(Rival et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24621

w¹; P{UAS-Cat.A}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Cat^Scer\UAS.cAa

Cat^Scer\UAS.cMa

Cat^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Anderson

Saccharomyces cerevisiae UAS construct a of Missirlis

(Missirlis et al., 2001)

Secondary FlyBase IDs

FBal0198446
FBal0191000

References (55)

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