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General Information
Symbol
Dmel\CatUAS.cAa
Species
D. melanogaster
Name
FlyBase ID
FBal0212304
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Cat, UAS-Catalase, UAS-Cat.A
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt sequences drive expression of Cat.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 
Global overexpression of Cat ameliorates the synaptic overgrowth phenotype but not the locomotion defects of park25/park25 larvae modelling Parkinson's disease.
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The size distribution of regenerated adult wings in flies expressing CatScer\UAS.cAa under the control of Scer\GAL4rn-GAL4-5 that were subjected to ablation of imaginal wing disc tissue during the larval stage, is comparable to that observed in similarly treated controls.
Expression of CatScer\UAS.cAa specifically in nonmyocytic pericardial cells, under the control of Scer\GAL4Dot.PK or Scer\GAL4sns.GCN results in a significant reduction in the level of physiological reactive oxygen species. Compared to wild-type controls, these flies exhibit a marked deterioration in the regularity of heart rhythm, with an increased frequency of irregular beats, a broader distribution of heart periods, and significantly increased arrhythmia. These flies also exhibit a narrowed heart tube phenotype at 1 week of age, as manifested by significant decreases in the diastolic and systolic diameters, compared to control hearts. By 4 weeks of age, the flies with reduced reactive oxygen species levels exhibit enlarged heart tubes compared with control hearts because of a significantly greater diastolic diameters. Expression of CatScer\UAS.cAa in cardiomyocytes and nonmyocytic pericardial cells, under the control of Scer\GAL4Hand.PA, which results in a significant reduction in the level of physiological reactive oxygen species, has no significant effects on any major aspects of heart function, including heart rhythm or heart tube dimensions. Overexpression of CatScer\UAS.cAa in nonmyocytic pericardial cells during embryogenesis and larval stages, under the control of Scer\GAL4Dot.PK and Scer\GAL80ts.αTub84B results in significant cardiac arrhythmias, compared to controls.
Animals expressing CatScer\UAS.cAa via Scer\GAL4da.PU are strikingly resistant to ethanol exposure compared to controls.
Expression of CatScer\UAS.cAa under the control of Scer\GAL4Hand.ΔVM.Switch (initiated by feeding flies RU486 during adulthood) improves cardiac performance. The heart period (mean time between successive end-diastolic positions) and frequency of arrhythmia are reduced in 45 day and 60 day old mutant flies compared to age-matched controls. Expression of CatScer\UAS.cAa also prevents the age-related decrease in End-Diastolic Diameter (EDD) seen in wild type flies; EDD remains stable between 10 days and 60 days.
Expression of CatScer\UAS.cAa under the control of Scer\GAL4Antp-10 reduces the increase in lamellocyte number seen following wasp infestation.
Expression of CatScer\UAS.cAa under the control of Scer\GAL4Act does not rescue the reduction in lifespan seen in flies under hyperoxia. Expression of CatScer\UAS.cAa under the control of Scer\GAL4ple.PF does not rescue the degeneration of dopaminergic neurons that is seen in flies under hyperoxia.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Expression of CatScer\UAS.cAa under the control of Scer\GAL4ninaE.PT fails to rescue the neurodegeneration and electrophysiology defect seen in fh1 mutant photoreceptor clones.
The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology defects in adult indirect flight muscles (enlarged clustered mitochondria) characteristic for animals expressing cluGD13926 under the control of Scer\GAL4Mef2.PR cannot be suppressed by co-expression of CatScer\UAS.cAa.
The co-expression of CatScer\UAS.cAa does not suppress the tumor phenotype and tissue disorganization resulting from the expression of dlg1HMS01954 under the control of Scer\GAL4Bx-MS1096.
The expression of vtdGL00522 under the control of Scer\GAL4en.PU (together with Dicer-2, for efficient vtd RNAi) leads to a significant increase in apoptosis in the posterior compartment of the third instar larval wing disc, which is suppressed by co-expressing CatScer\UAS.cAa. Expression of vtdGL00522 under the control of Scer\GAL4en.PU (together with Dicer-2, for efficient RNAi) results in increased numbers of hemocytes on the surface of third instar larval wing discs, as compared to controls, which are suppressed by co-expressing CatScer\UAS.cAa.
A p38b156A, p38a13 double mutant background partially restores a normal heart rhythm and normalizes the diastolic and systolic heart diameters of flies expressing CatScer\UAS.cAa under the control of Scer\GAL4Dot.PK.
Expression of CatScer\UAS.cAa fails to suppress the heart defects seen when fhdsRNA.IR.Scer\UAS is expressed under the control of Scer\GAL4Hand.ΔVM.Switch.
Co-expression of CatScer\UAS.cAa and PHGPxScer\UAS.cMa, under the control of Scer\GAL4GMR.PF rescues the cell death found after H[[2]]O[[2]] application on larval eye discs.
Expression of CatScer\UAS.cAa under the control of Scer\GAL4Act5C.PU suppresses the synaptic overgrowth, but not the locomotion defects of park25/park25 larvae. Expression of CatScer\UAS.cAa under the control of Scer\GAL4Act5C.PU does not rescue the muscle resting membrane potential of park25/parkZ3678 larvae.
Co-expression of CatScer\UAS.cAa under the control of Scer\GAL4GMR.PF almost completely suppresses the cln3Scer\UAS.cTa-overexpression phenotype in the eye.
Expression of CatScer\UAS.cAa under the control of Scer\GAL4Mef2.PR does not rescue the reduced viability seen in p38bΔ25 Mpk21 double mutants.
Xenogenetic Interactions
Statement
Reference
Overexpression of CatScer\UAS.cAa in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS necrotic neurons reduces the eye defect and JNK activation without affecting primary necrosis. Overexpression of CatScer\UAS.cAa and PHGPxScer\UAS.cMa in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS necrotic neurons reduces the eye defect and JNK activation without affecting primary necrosis.
Overexpression of CatScer\UAS.cAa in flies expressing Hsap\GFAPScer\UAS.cWa under the control of Scer\GAL4repo results in a significant reduction in cell death. In addition, expression of CatScer\UAS.cAa also decreases the number of seizures. Protection from cellular toxicity does not alter the number of inclusion bodies. Overexpression of CatScer\UAS.cAa in flies expressing Hsap\GFAPR79H.Scer\UAS under the control of Scer\GAL4repo results in a significant reduction in cell death. In addition, expression of CatScer\UAS.cAa also decreases the number of seizures. Protection from cellular toxicity does not alter the number of inclusion bodies.
Expression of CatScer\UAS.cAa moderately suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1Scer\UAS.cSa is expressed under the control of Scer\GAL4Hml.Δ.
Overexpression of CatScer\UAS.cAa suppresses the decreased survival phenotype of flies expressing Hsap\APPArctic.Scer\UAS.T:nec under the control of Scer\GAL4elav-C155. Overexpression of CatScer\UAS.cAa suppresses the decline in locomotor function of flies expressing Hsap\APPArctic.Scer\UAS.T:nec under the control of Scer\GAL4elav-C155. Overexpression of CatScer\UAS.cAa suppresses the decline in locomotor function of flies expressing Hsap\APPScer\UAS.T:nec under the control of Scer\GAL4elav-C155.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
CatScer\UAS.cAa
CatScer\UAS.cMa
CatUAS.cAa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Anderson
Saccharomyces cerevisiae UAS construct a of Missirlis
Secondary FlyBase IDs
  • FBal0198446
  • FBal0191000
References (38)