Allele Hsap\HSPA1L^{K71E.Scer\UAS}

General Information
Symbol	Hsap\HSPA1L^{K71E.Scer\UAS}	Species	H. sapiens
Name		FlyBase ID	FBal0212634
Feature type	allele	Associated gene	Hsap\HSPA1L

Allele class
Mutagen	in vitro construct - amino acid replacement, in vitro construct - site directed mutagenesis
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Bonini, 2002) in vitro construct - site directed mutagenesis (Bonini, 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Hsap\HSPA1L^Scer\UAS.cWa (Bonini, 2002)
Nature of the lesion	Statement Reference Encodes a dominant negative form of Hsap\HSPA1L. (Bonini, 2002)
Carried in construct	P{UAS-Hsp70.K71E} (Warrick et al., 2005, Bonini, 2002, Bilen and Bonini, 2007)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^GMR.PF (Warrick et al., 2005, Bonini, 2002) visible, with Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Warrick et al., 2005, Bonini, 2002) eye, with Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
Detailed Description
	Statement Reference Expression of Hsap\HSPA1L[K71E.Scer\UAS] under the control of Scer\GAL4[GMR.PU] results in degeneration of the eye. (Bilen and Bonini, 2007) Flies expressing Hsap\HSPA1L^{K71E.Scer\UAS} under the control of Scer\GAL4^GMR.PF have a severely disrupted eye. Co-expression of Hsap\HSPA1L^Scer\UAS.cWa partially suppresses the mutant eye phenotype caused by expression of Hsap\HSPA1L^{K71E.Scer\UAS} under the control of Scer\GAL4^GMR.PF. (Warrick et al., 2005) Flies expressing Hsap\HSPA1L^{K71E.Scer\UAS} under the control of Scer\GAL4^GMR.PF show loss of eye pigmentation and deterioration of eye structure. (Bonini, 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by CG5009^B227.2, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by DnaJ-1^B345.2, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by DnaJ-1^EP411, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Tpr2^EB7-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
NOT Enhanced by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by Ubp64E^E213-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
Suppressed by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, suppressible \| partially by CG11700^EP1384, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Warrick et al., 2005) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by CG8209^B3-Sa, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by CG11700^EP1384, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by CG14207^EP1348, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by dbr^EP9, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by dbr^EP456, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by emb^E2-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by emb^E128-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Faf^E659, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Hsp68^E407, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Imp^EP1433, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Kdm2^EP3093, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by mrj^E1050, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by NFAT^EP1335, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by NFAT^EP1508, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by orb2^B8-S, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Sin3A^B9-E, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by wech^E546, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by wech^EP2594, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by wech^JM120, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible by wech^JM265, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
NOT suppressed by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Hsap\ATXN3^{fl.Q27.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Warrick et al., 2005) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Ubp64E^E213-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
Other
Statement Reference DnaJ-1^B345.2, Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has lethal - all die during pupal stage phenotype (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU, Tpr2^EB7-1A has lethal - all die during pupal stage phenotype (Bilen and Bonini, 2007)
Phenotype Manifest In
Enhanced by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by CG5009^B227.2, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by DnaJ-1^B345.2, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by DnaJ-1^EP411, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Tpr2^EB7-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
NOT Enhanced by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Ubp64E^E213-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
Suppressed by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible \| partially by CG11700^EP1384, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Warrick et al., 2005) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by CG8209^B3-Sa, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by CG11700^EP1384, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by CG14207^EP1348, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by dbr^EP9, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by dbr^EP456, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by emb^E2-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by emb^E128-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Faf^E659, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Hsp68^E407, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Imp^EP1433, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Kdm2^EP3093, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by mrj^E1050, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by NFAT^EP1335, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by NFAT^EP1508, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by orb2^B8-S, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Sin3A^B9-E, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by wech^E546, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by wech^EP2594, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by wech^JM120, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by wech^JM265, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
NOT suppressed by
Statement Reference Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Hsap\ATXN3^{fl.Q27.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Warrick et al., 2005) Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Ubp64E^E213-1A, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Hsap\MJD^{fl.Q27.Scer\UAS.T:Hsap\MYC} has little or no effect on the mutant eye phenotype caused by expression of Hsap\HSPA1L^{K71E.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Co-expression of CR11700^EP1384 partially suppresses the mutant eye phenotype caused by expression of Hsap\HSPA1L^{K71E.Scer\UAS} under the control of Scer\GAL4^GMR.PF. (Warrick et al., 2005)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\HSPA1L^{K71E.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Bilen and Bonini, 2007, PLoS Genet. 3(10): e177 Genome-wide screen for modifiers of ataxin-3 neurodegeneration in Drosophila. [FBrf0202746] Warrick et al., 2005, Mol. Cell 18(1): 37--48 Ataxin-3 suppresses polyglutamine neurodegeneration in Drosophila by a ubiquitin-associated mechanism. [FBrf0188095]
Review	Bonini, 2002, Proc. Natl. Acad. Sci. U.S.A. 99(Suppl. 4): 16407--16411 Chaperoning brain degeneration. [FBrf0159309]

Allele Hsap\HSPA1LK71E.Scer\UAS

Allele Hsap\HSPA1L^{K71E.Scer\UAS}