lethal, with Scer\GAL4T80
eye, with Scer\GAL4ey.PH
eye, with Scer\GAL4GMR.PF
wing, with Scer\GAL4sd.PU
wing | posterior, with Scer\GAL4en-e16E
Knock-down of kay in the wing (via Scer\GAL4sd.PU-mediated expression of kaydsRNA.Scer\UAS) results in partial lethality and in uninflated wings in the survivors.
Expression of kaydsRNA.Scer\UAS in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) results in a significant decrease in RP2 dendrite volume compared to controls.
Expression of kaydsRNA.Scer\UAS under the control of Scer\GAL4ey.PH at 25[o]C results in rough eyes that are smaller than the eyes of wild-type flies. This phenotype shows variable penetrance; in some flies, the eye is completely ablated. When flies of this genotype are raised at 29[o]C, the eye discs of third-instar imaginal larvae remain extremely small and are fragile.
Expression of kaydsRNA.Scer\UAS under the control of Scer\GAL4en-e16E results in pupal lethality when flies are raised at 25[o]C. At lower temperatures, a small proportion of pupae hatch into adult flies. The posterior wing compartment where the transgene is expressed is smaller than in control flies, while the anterior compartment, where the transgene is not expressed, is of normal size.
Expression of kaydsRNA.Scer\UAS throughout the wing, driven by Scer\GAL4sd-SG29.1, results in the entire wing being small and malformed.
Cells expressing kaydsRNA.Scer\UAS under the control of Scer\GAL4en-e16E are larger than control cells. An increased fraction of these cells are at the G[[2]]M phase of the cell cycle, while the fraction of cells in G[[1]] is small in comparison to controls. Many cells in the affected area undergo apoptosis. Cells expressing kaydsRNA.Scer\UAS for a short period of time only, under the control of Scer\GAL4T80, also show an accumulation in the G[[2]]/M phase of the cell cycle.
Eye discs expressing kaydsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF show a distinctive stripe of apoptosis immediately posterior to the second mitotic wave that is not seen in wild-type eye discs. Eyes emerging from Scer\GAL4GMR.PF>kaydsRNA.Scer\UAS larvae are smaller than wild-type eyes and do not show the regular ommatidial pattern seen in wild type. Many ommatidia are fused as insufficient numbers of pigment cells are present to separate the ommatidia.
Scer\GAL4sd.PU, kayRNAi.UAS has partially lethal phenotype, suppressible | partially by CRIFEY03252/CG7172[+]
Scer\GAL4Act5C.PI/kayRNAi.UAS is an enhancer of neoplasia | somatic clone phenotype of scrib1
kayRNAi.UAS, Scer\GAL4GMR.PF is a non-suppressor of visible phenotype of Atf3UAS.cSa, Scer\GAL4GMR.PF
kayRNAi.UAS, Scer\GAL4ppl.PP is a non-suppressor of visible phenotype of Atf3UAS.cSa, Scer\GAL4ppl.PP
Scer\GAL4GMR.PF, kayRNAi.UAS has eye phenotype, enhanceable by BacA\p35UAS.cHa, Scer\GAL4GMR.PF
Scer\GAL4sd.PU, kayRNAi.UAS has wing phenotype, suppressible | partially by CRIFEY03252/CG7172[+]
Scer\GAL4Act5C.PI/kayRNAi.UAS is an enhancer of eye-antennal disc | somatic clone phenotype of scrib1
kayRNAi.UAS, Scer\GAL4slbo.2.6 is a suppressor | partially of border follicle cell phenotype of PvrDN.UASp, Scer\GAL4slbo.2.6, hepAct.UAS
kayRNAi.UAS, Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of Atf3UAS.cSa, Scer\GAL4GMR.PF
kayRNAi.UAS, Scer\GAL4ppl.PP is a non-suppressor of adult abdomen | dorsal phenotype of Atf3UAS.cSa, Scer\GAL4ppl.PP
kayRNAi.UAS, Scer\GAL4ppl.PP is a non-suppressor of adult cuticle | dorsal phenotype of Atf3UAS.cSa, Scer\GAL4ppl.PP
The increased cell proliferation observed upon expression of either hepScer\UAS.cBa or Ras85DV12.Scer\UAS under the control of Scer\GAL4esg-NP5130 in the adult gut is suppressed by co-expression of kaydsRNA.Scer\UAS. Similarly, the Sox21aScer\UAS.T:Zzzz\FLAG expression-induced increase in proliferation is partially suppressed by co-expression of kaydsRNA.Scer\UAS when an insertion line with a strong expression is used but has no significant effect when a weak-expressing line is used.
The mutant eye phenotype caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is not suppressed by co-expression of kaydsRNA.Scer\UAS.
Co-expression of kaydsRNA.Scer\UAS does not suppress the dorsal cleft in the abdominal cuticle caused by expression of Atf3Scer\UAS.cSa under the control of Scer\GAL4ppl.PP.
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of kaydsRNA.Scer\UAS.
At 22[o]C, Scer\GAL4en-e16E>kaydsRNA.Scer\UAS expression does not affect adult wing size in a wild-type background but the size of the posterior compartment is reduced when this transgene is expressed in a sro1/+ background.
scrib1 eye/antennal imaginal disc clones grow larger in size when they express kaydsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PI. Apoptosis is suppressed in the Scer\GAL4Act5C.PI>kaydsRNA.Scer\UAS; scrib1 clones.
Coexpression of BacA\p35Scer\UAS.cHa, which inhibits apoptosis, with kaydsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF results in a more severe phenotype in the eye than when kaydsRNA.Scer\UAS is expressed alone, as ommatidial organization is completely disrupted.
Expression of P{UAS-kay.RNAi} produces dsRNA which results in dsRNA interference (RNAi) of the kay gene.