FB2025_01 , released February 20, 2025
Allele: Dmel\Mmp2RNAi.UAS
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General Information
Symbol
Dmel\Mmp2RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0212916
Feature type
allele
Associated gene
Dmel\Mmp2
Associated Insertion(s)
Carried in Construct
P{UAS-Mmp2.RNAi}
Also Known As
UAS-mmp2RNAi, UAS-Mmp2-RNAi
Key Links
Transgenic product class
RNAi_reagent
(Uhlirova and Bohmann, 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Uhlirova and Bohmann, 2006)
Mutagen
in vitro construct
(Uhlirova and Bohmann, 2006)
Progenitor genotype
Carried in construct
P{UAS-Mmp2.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of Mmp2. The repeat is separated by a w intron.

(Uhlirova and Bohmann, 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mmp2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  intestinal cancer
      modeled by Sox21a6
      (Zhai et al., 2015)
      ameliorates  carcinoma
      modeled by Ras85DV12.UAS, scrib1
      (Uhlirova and Bohmann, 2006)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stage 14 egg chambers from females expressing Mmp2dsRNA.UAS under the control of Scer\GAL4GMR47A04 show an intact or broken posterior basement membrane, instead of the mostly open posterior basement membrane observed in controls.

      (Deady et al., 2017)

      Knockdown of Mmp2dsRNA.Scer\UAS driven by Scer\GAL4Act.PU during adulthood (using Scer\GAL80ts.αTub84B) causes a significant reduction in egg laying compared to wild type; mature egg chambers accumulate, ovaries lack corpus luteum structures and average time to ovulate significantly increases. Follicle cell trimming significantly decreases and the number of eggs laid in 6 hours after mating significantly decreases in Scer\GAL4Act.PU>Mmp2dsRNA.Scer\UAS (with Scer\GAL80ts.αTub84B) females.

      Expression of Mmp2dsRNA.Scer\UAS driven by Scer\GAL4GMR47A04 (along with UAS-dcr2) leads to a significant decrease in egg laying and a significant increase in ovulation time in females, compared to controls. Scer\GAL4GMR42A05>Mmp2dsRNA.Scer\UAS females lay significantly fewer eggs (in 2 days) compared to controls.

      (Deady et al., 2015)

      Expression of Mmp2dsRNA.Scer\UAS under the control of Scer\GAL4btl.PS results in increased basal lamina deposition around tunneled tracheae and dorsal air sac primordia associated with the wing disc. Extension and growth of the dorsal air sac primordia is stunted in these mutants, and the developing air sac tubes are filled with polarised epithelial cells.

      Expression of Mmp2dsRNA.Scer\UAS in the wing imaginal disc under the control of Scer\GAL4ap-md544 causes hyperplastic growth of the dorsal air sac primordia.

      (Guha et al., 2009)

      Expression of Mmp2dsRNA.Scer\UAS under the control of Scer\GAL4pnr-MD237 results in a cleft thorax phenotype.

      (Uhlirova and Bohmann, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Scer\GAL4Su(H).GBE/Mmp2RNAi.UAS is a suppressor | partially of neoplasia phenotype of Sox21a6

      (Zhai et al., 2015)
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The disrupted formation of the presumptive posterior crossvein (assessed by p-Mad staining) in the pupal wing observed in animals expressing Gyc76CVDRC.cUa under the control of Scer\GAL4hh.PU is rescued by co-expression of Mmp2dsRNA.Scer\UAS, although the Mmp2dsRNA.Scer\UAS,Gyc76CVDRC.cUa double knock-down larvae and pupae are occasionally unhealthy-looking and those individuals also have pupal wings of poor morphology.

      (Schleede and Blair, 2015)

      Expression of Mmp2dsRNA.Scer\UAS in enteroblasts under the control of Scer\GAL4Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a6 mutant flies.

      (Zhai et al., 2015)

      Expression of either Mmp2dsRNA.Scer\UAS in Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, scrib1 eye/antennal imaginal disc clones significantly suppresses the cell invasiveness phenotype but does not suppress the formation of tumors within the disc. Coexpression of Mmp1dsRNA.Scer\UAS and Mmp2dsRNA.Scer\UAS further reduces the invasiveness of Scer\GAL4Act5C.PI>Ras85DV12.Scer\UAS, scrib1 cells.

      (Uhlirova and Bohmann, 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Expression of P{UAS-Mmp2.RNAi} produces dsRNA which results in dsRNA interference (RNAi) of the Mmp2 gene.

      (Uhlirova and Bohmann, 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Mmp2RNAi.UAS
      Mmp2dsRNA.Scer\UAS
      Mmp2dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (13)