Allele Dmel\tok^D427

General Information
Symbol	Dmel\tok^D427	Species	D. melanogaster
Name		FlyBase ID	FBal0212941
Feature type	allele	Associated gene	Dmel\tok

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Meyer and Aberle, 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q464@. (Meyer and Aberle, 2006)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (with tok^K788) (Meyer and Aberle, 2006)
Phenotype Manifest In
	intersegmental nerve (with tok^K788) (Meyer and Aberle, 2006) intersegmental nerve \| embryonic stage (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal dorsal acute muscle 1 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal dorsal acute muscle 2 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal dorsal oblique muscle 1 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal dorsal oblique muscle 2 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal lateral transverse muscle 1 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal lateral transverse muscle 2 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal lateral transverse muscle 3 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal lateral transverse muscle 4 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral acute muscle 1 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral acute muscle 2 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral acute muscle 3 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral longitudinal muscle 1 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral longitudinal muscle 2 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral longitudinal muscle 3 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral longitudinal muscle 4 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral oblique muscle 4 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral oblique muscle 5 (with tok^K788) (Meyer and Aberle, 2006) larval neuromuscular junction & abdominal ventral oblique muscle 6 (with tok^K788) (Meyer and Aberle, 2006) presumptive embryonic/larval central nervous system (with tok^K788) (Meyer and Aberle, 2006) segmental nerve (with tok^K788) (Meyer and Aberle, 2006) segmental nerve \| embryonic stage (with tok^K788) (Meyer and Aberle, 2006)
Detailed Description
	Statement Reference The ISN motor nerve of tok^K788/tok^D427 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 38.1% (muscle 1), 25.0% (muscle 9), 7.1% (muscle 2), 3.6% (muscle 10). The SNa motor nerve of tok^K788/tok^D427 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 8.4% (muscle 21), 1.2% (muscle 22), 1.2% (muscle 23), 34.5% (muscle 24). The ISNb motor nerve of tok^K788/tok^D427 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 64.3% (muscle 12), 56.0% (muscle 13), 23.9% (muscle 6), 34.6% (muscle 7). The SNc motor nerve of tok^K788/tok^D427 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 21.7% (muscle 26), 74.7% (muscle 27), 71.1% (muscle 29). The ISNd motor nerve of tok^K788/tok^D427 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 83.4% (muscle 15), 27.4% (muscle 16), 34.6% (muscle 17). In tok^K788/tok^D427 third instar larvae, muscles 12 and 13 are often innervated via a dorsoventral projection of the ISN or SNa nerves, which induces the formation of neuromuscular junctions at ectopic sites (in wild-type larvae these muscles are innervated by a ventrodorsal projection of the ISNb pathway). The ISNb nerve frequently remains attached to the ISN in tok^K788/tok^D427 larvae, sometimes as a separately identifiable axon bundle, and reaches its target muscles via irregular routes. Defasciculation defects are also seen in the SN pathway, with the SNa nerve often growing more posteriorly than normal as it defasciculates too late. The branch points of the ISN appear underdeveloped in tok^K788/tok^D427 embryos, and/or the ISN is stalled at the second branch point and barely reaches the position of the third branch point. 51.2% of hemisegments have SNa defects in these embryos, including two dorsal branches of the SNa. The ISNb fails to defasciculate from the ISN in 96.2% of hemisegments. It is either tightly attached to the ISN or forms a separate parallel nerve bundle. Defasciculation defects are also see in Fas2-expressing nerve tracts in the embryonic central nervous system. (Meyer and Aberle, 2006)
External Data
Linkouts
Interactions
Phenotypic Class
NOT suppressed by
Statement Reference tok^K788/tok^D427 has neuroanatomy defective phenotype, non-suppressible by kuz^Scer\UAS.cFa/Scer\GAL4^elav.PLu (Meyer and Aberle, 2006) tok^K788/tok^D427 has neuroanatomy defective phenotype, non-suppressible by tld^Scer\UAS.cHa/Scer\GAL4^elav.PLu (Meyer and Aberle, 2006)
Phenotype Manifest In
NOT suppressed by
Statement Reference tok^K788/tok^D427 has embryonic/larval neuromuscular junction phenotype, non-suppressible by kuz^Scer\UAS.cFa/Scer\GAL4^elav.PLu (Meyer and Aberle, 2006) tok^K788/tok^D427 has embryonic/larval neuromuscular junction phenotype, non-suppressible by tld^Scer\UAS.cHa/Scer\GAL4^elav.PLu (Meyer and Aberle, 2006)
Additional Comments
Genetic Interactions
Statement Reference tok^K788 side^C137/tok^D427 side^C137 double mutant larvae show a strong enhancement of each single mutant phenotype; double mutant larvae lack virtually all neuromuscular junctions on all ventral muscles in all abdominal hemisegments. Dorsal and lateral muscles also show an increase in innervation errors compared to single mutants. (Meyer and Aberle, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	tok^K788/tok^D427 is rescued by Scer\GAL4^G14/tok^{Scer\UAS.cSa.T:Ivir\HA1} (Meyer and Aberle, 2006) tok^K788/tok^D427 is rescued by Scer\GAL4^srp/tok^{Scer\UAS.cSa.T:Ivir\HA1} (Meyer and Aberle, 2006) tok^K788/tok^D427 is rescued by tok^{Scer\UAS.cSa.T:Ivir\HA1}/Scer\GAL4^elav.PLu (Meyer and Aberle, 2006) tok^K788/tok^D427 is rescued by tok^{Scer\UAS.cSa.T:Ivir\HA1}/Scer\GAL4^hs.PB (Meyer and Aberle, 2006) tok^K788/tok^D427 is rescued by tok^{Scer\UAS.cSa.T:Ivir\HA1}/Scer\GAL4^ppl.PP (Meyer and Aberle, 2006)
Not rescued by	tok^K788/tok^D427 is not rescued by tok^{Scer\UAS.cSa.T:Ivir\HA1}/Scer\GAL4^Cha.7.4 (Meyer and Aberle, 2006)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Selected as: A mutation affecting the structure of the neuromuscular junction. (Meyer and Aberle, 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	piranha^D427 (Meyer and Aberle, 2006) tlr1^D427 (Meyer and Aberle, 2006) tok^D427
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Meyer and Aberle, 2006, Development 133(20): 4035--4044 At the next stop sign turn right: the metalloprotease Tolloid-related 1 controls defasciculation of motor axons in Drosophila. [FBrf0193012]

Allele Dmel\tokD427

Allele Dmel\tok^D427