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General Information
Symbol
Dmel\sideI1563
Species
D. melanogaster
Name
FlyBase ID
FBal0212946
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

growth cone & intersegmental nerve (with sideC137)

larval neuromuscular junction & abdominal dorsal acute muscle 1 (with sideC137)

larval neuromuscular junction & abdominal dorsal acute muscle 2 (with sideC137)

larval neuromuscular junction & abdominal dorsal oblique muscle 1 (with sideC137)

larval neuromuscular junction & abdominal dorsal oblique muscle 2 (with sideC137)

larval neuromuscular junction & abdominal lateral transverse muscle 1 (with sideC137)

larval neuromuscular junction & abdominal lateral transverse muscle 2 (with sideC137)

larval neuromuscular junction & abdominal lateral transverse muscle 3 (with sideC137)

larval neuromuscular junction & abdominal lateral transverse muscle 4 (with sideC137)

larval neuromuscular junction & abdominal ventral acute muscle 1 (with sideC137)

larval neuromuscular junction & abdominal ventral acute muscle 2 (with sideC137)

larval neuromuscular junction & abdominal ventral acute muscle 3 (with sideC137)

larval neuromuscular junction & abdominal ventral longitudinal muscle 1 (with sideC137)

larval neuromuscular junction & abdominal ventral longitudinal muscle 2 (with sideC137)

larval neuromuscular junction & abdominal ventral longitudinal muscle 3 (with sideC137)

larval neuromuscular junction & abdominal ventral longitudinal muscle 4 (with sideC137)

larval neuromuscular junction & abdominal ventral oblique muscle 4 (with sideC137)

larval neuromuscular junction & abdominal ventral oblique muscle 5 (with sideC137)

larval neuromuscular junction & abdominal ventral oblique muscle 6 (with sideC137)

Detailed Description
Statement
Reference

49% of hemisegments show detachment of ISN motor axons from sensory nerves of the anterior fascicle in sideC137/sideI1563 embryos.

sideC137/sideI1563 embryos show severe delays in the dorsal migration of the ISN compared to wild type. Growth cones do eventually manage to cross the dorsal trunk, but a subset lag behind. The average growth rate of the mutant nerve is 15.2 +/- 4.6 mm/h compared to 31.9 +/- 13.4 mm/h for wild-type nerves. A small fraction of mutant ISN growth cones show highly disoriented directionality, extending in abnormal directions over the course of axonal growth.

Most, if not all, ISN growth cones are tightly attached to tracheal branches in sideC137/sideI1563 embryos expressing sideScer\UAS.cSa in the trachea under the control of Scer\GAL4btl.PS. The ISN follows the tracheal substrate in these embryos and crosses and re-crosses the transverse connective.

Most, if not all, ISN growth cones are attached to hemocytes in sideC137/sideI1563 embryos expressing sideScer\UAS.cSa in the hemocytes under the control of Scer\GAL4srp (this does not occur in wild-type embryos). Filopodial extensions of both cell membranes are tightly interdigitated.

sideC137/sideI1563 third instar larvae often lack neuromuscular junctions (NMJs) on dorsal muscles (the percentage of muscles lacking NMJs is given in parentheses); muscle 1 (19%), muscle 9 (24%), muscle 2 (11%), muscle 10 (9%).

sideC137/sideI1563 third instar larvae often lack neuromuscular junctions (NMJs) on ventral muscles (the percentage of muscles lacking NMJs is given in parentheses); muscle 12 (58%), muscle 13 (75%), muscle 6 (77%), muscle 7 (85%).

The ISN motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 25.0% (muscle 1), 35.0% (muscle 9), 16.7% (muscle 2), 11.7% (muscle 10).

The SNa motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 41.7% (muscle 21), 3.4% (muscle 22), 3.3% (muscle 23), 51.7% (muscle 24).

The ISNb motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 75.0% (muscle 12), 75.0% (muscle 13), 83.3% (muscle 6), 85.0% (muscle 7).

The SNc motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 61.7% (muscle 26), 86.7% (muscle 27), 66.7% (muscle 29).

The ISNd motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 78.3% (muscle 15), 63.3% (muscle 16), 98.4% (muscle 17).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: A mutation affecting the structure of the neuromuscular junction.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)