Allele Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP}	Species	H. sapiens
Name		FlyBase ID	FBal0212958
Feature type	allele	Associated gene	Hsap\PPAP2B

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Burnett and Howard, 2003) in vitro construct - regulatory fusion (Burnett and Howard, 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\PPAP2B tagged at the C-terminal end with T:Avic\GFP^EGFP. (Burnett and Howard, 2003)
Carried in construct	P{UAS-hLPP3.EGFP} (Renault et al., 2004, Burnett and Howard, 2003, Renault et al., 2004)
Tagged with	T:Avic\GFP^EGFP (Burnett and Howard, 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	germline cell, with Scer\GAL4^twi.PG (Burnett and Howard, 2003)
Detailed Description
	Statement Reference Embryos expressing Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^twi.PG show a reduction in the number of primordial germ cells (PGCs) and defects in PGC migration. (Burnett and Howard, 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^{nos.UTR.T:Hsim\VP16}/Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP} is a suppressor \| partially of germline cell phenotype of wun2^{αTub84B.T:Hsap\MYC} (Renault et al., 2004) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP} is a suppressor of germline cell \| maternal effect phenotype of wun2^EP2650ex34/Df(2R)wun-GL (Renault et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference The germ cell death seen in embryos with a wun2⁺ soma which are derived from wun2^EP2650ex34/Df(2R)wun-GL females is rescued by expression of Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. Expression of Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the germ cells partially suppresses the reduction in germ cell number seen in embryos expressing wun2^{αTub84B.T:Hsap\MYC} in the soma. (Renault et al., 2004)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in plasmid "Human LPP3-GFP", transfected into S2 cells and used to study the catalytic activity of the protein produced. (Burnett and Howard, 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Renault et al., 2004, Science 305(5692): 1963--1966 Soma-germ line competition for lipid phosphate uptake regulates germ cell migration and survival. [FBrf0180678] Burnett and Howard, 2003, EMBO Rep. 4(8): 793--799 Fly and mammalian lipid phosphate phosphatase isoforms differ in activity both in vitro and in vivo. [FBrf0162096]
Supplementary material	Renault et al., 2004, Science 305(5692): Supporting Online Material. [FBrf0202565]

Allele Hsap\PPAP2BScer\UAS.T:Avic\GFP-EGFP

Allele Hsap\PPAP2B^{Scer\UAS.T:Avic\GFP-EGFP}