Allele Dmel\Hs6st^d770

General Information
Symbol	Dmel\Hs6st^d770	Species	D. melanogaster
Name		FlyBase ID	FBal0212981
Feature type	allele	Associated gene	Dmel\Hs6st

Allele class	loss of function allele
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Kamimura et al., 2006)
Mutagen	Delta2-3 (Kamimura et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Hs6st^A201.1M3 (Kamimura et al., 2006)
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	embryonic/larval trachea (Kamimura et al., 2006) imaginal tracheoblast (Kamimura et al., 2006) presumptive embryonic/larval tracheal system (Kamimura et al., 2006)
Detailed Description
	Statement Reference Zygotic Hs6st^d770 mutants survive to the adult stage without showing obvious morphological defects. A fraction (39%) of maternal and zygotic Hs6st^d770 mutants exhibit abnormal tracheal development. Tracheal development is incomplete, revealed by the presence of large gaps in the dorsal trunks, as well as stalled tracheal branches. The migration defects in these embryos are observed in all primary branches, but most commonly in the dorsal branch and the dorsal trunk. Tracheal morphology is indistinguishable from that of wild-type embryos in the remaining 61% of embryos. Normal development of tracheoblasts is observed in the majority of Hs6st^d770 mutants, although the tracheoblast is slightly reduced in size in a small fraction (18%) of Hs6st^d770 mutant discs. (Kamimura et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Hs6st^d770 is a suppressor of visible phenotype of Sulf1^ΔP1 (Kleinschmit et al., 2010)
Other
Statement Reference Hs2st^d267, Hs6st^d770 has lethal phenotype (Kamimura et al., 2006) Hs2st^d267, Hs6st^d770 has partially lethal - majority die phenotype (Kamimura et al., 2006)
Phenotype Manifest In
Suppressor of
Statement Reference Hs6st^d770 is a suppressor of anterior wing margin phenotype of Sulf1^ΔP1 (Kleinschmit et al., 2010) Hs6st^d770 is a suppressor of wing margin bristle \| supernumerary phenotype of Sulf1^ΔP1 (Kleinschmit et al., 2010)
Other
Statement Reference Hs2st^d267, Hs6st^d770 has tracheal air sac phenotype (Kamimura et al., 2006) Hs2st^d267, Hs6st^d770 has tracheal precursor cell phenotype (Kamimura et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Hs6st[d770] suppresses the increased chemosensory bristle phenotype observed at the anterior dorsal wing margin of Sulf1[ΔP1] mutant animals. (Kleinschmit et al., 2010) Embryos that are maternal and zygotic mutant for Hs6st^d770 and Hs2st^d267 are partial lethal during development. However, significant fractions of these null mutants survive to the adult stage without visible phenotypes. Hs2st^d267/Hs6st^d770 zygotic double mutants are completely lethal. Although invagination seems to occur normally in Hs2st^d267/Hs6st^d770 embryos, they exhibit several characteristic defects in branching morphogenesis. First, mutant tracheal precursor cells fail to migrate to form the primary branches. Second, clusters of mutant tracheal cells tend to extend dorsally and ventrally, forming long, skinny sacs of tracheal precursor cells of various sizes. Finally, approximately 16% of mutant embryos show fusion of the tracheal sacs to those in the neighboring segments. (Kamimura et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Hs6st^d770 is rescued by Scer\GAL4^btl.PS/Hs6st^Scer\UAS.cKa (Kamimura et al., 2006)
Comments	The small tracheoblast phenotype of Hs6st^d770 mutants is completely rescued by expression of Hs6st^Scer\UAS.cKa under the control of Scer\GAL4^btl.PS. (Kamimura et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hs6st^d770 (Kamimura et al., 2006, Kleinschmit et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Kleinschmit et al., 2010, Dev. Biol. 345(2): 204--214 Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation. [FBrf0211640] Kamimura et al., 2006, J. Cell Biol. 174(6): 773--778 Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling. [FBrf0193661]

Allele Dmel\Hs6std770

Allele Dmel\Hs6st^d770