Allele Dmel\Hs6st^Scer\UAS.cKa

General Information
Symbol	Dmel\Hs6st^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Kamimura	FlyBase ID	FBal0212983
Feature type	allele	Associated gene	Dmel\Hs6st

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kamimura et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS drives expression of a Hs6st cDNA. (Kamimura et al., 2011) Scer\UAS regulatory sequences drive expression of Hs6st coding sequences. (Kamimura et al., 2006)
Carried in construct	P{UAS-Hs6st.K} (Kamimura et al., 2006, Kamimura et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	partially lethal - majority die, with Scer\GAL4^Act.PU (Kamimura et al., 2011) partially lethal - majority die, with Scer\GAL4^hh.PU (Kamimura et al., 2011) size defective, with Scer\GAL4^hh.PU (Kamimura et al., 2011)
Phenotype Manifest In
	wing disc, with Scer\GAL4^hh.PU (Kamimura et al., 2011) wing margin, with Scer\GAL4^hh.PU (Kamimura et al., 2011) wing margin bristle, with Scer\GAL4^Act.PU (Kamimura et al., 2011)
Detailed Description
	Statement Reference Expression of Hs6st[Scer\UAS.cKa] using Scer\GAL4[Act.PU] does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system. Expression of Hs6st[Scer\UAS.cKa] using Scer\GAL4[hs.PU] and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality. Expression of Hs2st[Scer\UAS.cKa] with Scer\GAL4[Act.PU] results in 89% lethality, and with Scer\GAL4[hh.PU] results in 88% lethality. Expression of Hs6st[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in posterior wing margin notching in 14% of flies. Expression of Hs6st[Scer\UAS.cKa] using Scer\GAL4[Act.PU] impairs development of the chemosensory bristles at the wing margin. Expression of Hs6st[Scer\UAS.cKa] using Scer\GAL4[hh.PU] significantly decreases the size of the posterior compartment of the wing disc. (Kamimura et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hs6st^Scer\UAS.cKa, Scer\GAL4^Act.PU has partially lethal - majority die phenotype, suppressible by Hsepi^Scer\UAS.cKa, Scer\GAL4^Act.PU (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Scer\GAL4^hh.PU has partially lethal - majority die phenotype, suppressible by Hsepi^Scer\UAS.cKa, Scer\GAL4^hh.PU (Kamimura et al., 2011)
Other
Statement Reference Hs6st^Scer\UAS.cKa, Scer\GAL4^Act.PU, sfl^Scer\UAS.cKa has partially lethal - majority die phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has lethal phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has size defective phenotype (Kamimura et al., 2011)
Phenotype Manifest In
Other
Statement Reference Hs6st^Scer\UAS.cKa, Hsepi^Scer\UAS.cKa, Scer\GAL4^hh.PU has anterior crossvein phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Hsepi^Scer\UAS.cKa, Scer\GAL4^hh.PU has posterior crossvein phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Hsepi^Scer\UAS.cKa, Scer\GAL4^hh.PU has wing margin phenotype (Kamimura et al., 2011) Hs6st^Scer\UAS.cKa, Scer\GAL4^hh.PU, sfl^Scer\UAS.cKa has wing disc phenotype (Kamimura et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Simultaneous expression of Hs6st[Scer\UAS.cKa] and sfl[Scer\UAS.cKa] using Scer\GAL4[Act.PU] does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system. Simultaneous expression of Hs6st[Scer\UAS.cKa] and sfl[Scer\UAS.cKa] using Scer\GAL4[hs.PU] and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality. Co-expression of Hsepi[Scer\UAS.cKa] with Hs6st[Scer\UAS.cKa] using Scer\GAL4[Act.PU] results in 62% lethality. Co-expression of Hs6st[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[Act.PU] results in 69% lethality. Co-expression of Hsepi[Scer\UAS.cKa] with Hs6st[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in 57% lethality. Co-expression of Hs6st[Scer\UAS.cKa] with sfl[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in 100% lethality. Co-expression of Hs6st[Scer\UAS.cKa] with Hsepi[Scer\UAS.cKa] using Scer\GAL4[hh.PU] results in posterior wing margin notching in 4% of flies, loss of the anterior crossvein in 4 % of flies, and ectopic posterior cross vein tissue in 29% of flies. Co-expression of sfl[Scer\UAS.cKa] and Hs6st[Scer\UAS.cKa] using Scer\GAL4[hh.PU] significantly decreases the size of the posterior compartment of the wing disc. (Kamimura et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^btl.PS/Hs6st^Scer\UAS.cKa rescues Hs6st^d770 (Kamimura et al., 2006)
Comments	The small tracheoblast phenotype of Hs6st^d770 mutants is completely rescued by expression of Hs6st^Scer\UAS.cKa under the control of Scer\GAL4^btl.PS. (Kamimura et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Hs6st^Scer\UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Kamimura
Secondary FlyBase IDs

References ( 2 )
Research paper	Kamimura et al., 2011, Glycobiology 21(5): 607--618 In vivo manipulation of heparan sulfate structure and its effect on Drosophila development. [FBrf0213406] Kamimura et al., 2006, J. Cell Biol. 174(6): 773--778 Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling. [FBrf0193661]

Allele Dmel\Hs6stScer\UAS.cKa

Allele Dmel\Hs6st^Scer\UAS.cKa