FlyBase Allele Report: Hsap\BACE1[UAS.cGa]

General Information

Symbol

Hsap\BACE1^UAS.cGa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Greeve

FlyBase ID

FBal0213065

Feature type

allele

Associated gene

Hsap\BACE1

Associated Insertion(s)

Carried in Construct

P{UAS-BACE1.G}

Also Known As

UAS-BACE, P{UAS:BACE}, UAS-BACE1

Key Links

Transgenic product class

wild_type

(Greeve et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Greeve et al., 2004)

Mutagen

in vitro construct

(Greeve et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-BACE1.G}

Cytology

Description

UASt regulatory sequences drive expression of a 1.9kb KpnI-XhoI fragment of Hsap\BACE1, which contains the entire open reading frame.

(Greeve et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\BACE1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Chakraborty et al., 2011)

CEA with Hsap\APP^695.UAS

(Zhang et al., 2022, Miguel et al., 2020, Mhatre et al., 2014, Rajendran et al., 2008, Greeve et al., 2004)

CEA with Hsap\APP^695.UAS, Psn^+14.UAS

(Rajendran et al., 2008)

CEA with Hsap\APP^UAS.cUa

(Saburova et al., 2017)

model of attention deficit hyperactivity disorder

CEA with Hsap\APP^695.UAS

(Zhang et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

One copy of Df(2R)Egfr5 exacerbates the neurodegeneration seen in Alzheimer's disease modeled by expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa.

(Muhammad et al., 2008)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

axon | adult stage, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

eye photoreceptor cell, with Scer\GAL4^elav-C155

(Tsuda et al., 2010)

melanotic mass | adult stage, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

wing, with Scer\GAL4^elav-C155

(Chakraborty et al., 2011)

Detailed Description

Statement

Reference

No body wall contraction defects are seen in third instar larvae expressing Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155. Larvae exhibit a significant reduction in crawling distance and rate compared to controls.

(Mhatre et al., 2014)

Expression of Hsap\BACE1^Scer\UAS.cGa driven pan-neuronally by Scer\GAL4^elav-C155 results in crumpled wings (in 61% of flies) and presence of melanotic masses on the abdomen and proboscis (26%); penetrance is around tenfold higher than is seen with expression of Hsap\APP^695.Scer\UAS. Compared to genetic controls, Scer\GAL4^elav-C155>Hsap\BACE1^Scer\UAS.cGa young adults do not show significantly changes in survival.

Fly brains with expression of Hsap\BACE1^Scer\UAS.cGa driven by Scer\GAL4^elav-C155 show significant neuroanatomical changes: mushroom body calyx and lobe areas are significantly smaller than controls (or flies with expression of Hsap\APP^695.Scer\UAS or Hsap\BACE1^Scer\UAS.cLa) and Kenyon cell area is significantly smaller than is seen with expression of Hsap\BACE1^Scer\UAS.cLa; in many cases, axons extending to the alpha lobes are shorter or missing. Flies (2-10 and 12-20 days old) with expression of Hsap\BACE1^Scer\UAS.cGa driven by Scer\GAL4^elav-C155 show a significant decrease in climbing ability compared to controls. Flies with expression of Hsap\BACE1^Scer\UAS.cGa driven by Scer\GAL4^elav-C155 show normal initial learning in a courtship conditioning paradigm, but unlike controls do not display short term memory (tested 2 minutes post training).

(Chakraborty et al., 2011)

Expression of Hsap\BACE1^Scer\UAS.cGa in photoreceptor neurons under the control of Scer\GAL4^elav-C155 results in the degeneration of the highly organised architecture of retinal photoreceptors in an age-dependent manner.

(Tsuda et al., 2010)

Individuals simultaneously heterozygous for Df(2R)Egfr5 and expressing Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act.PU are short lived compared to controls.

(Muhammad et al., 2008)

Expression of Hsap\BACE1^Scer\UAS.cGa in eyes under the control of Scer\GAL4^GMR.PF does not cause retinal degeneration.

(Greeve et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal locomotor behavior | third instar larval stage phenotype, enhanceable by Hsap\APP^695.UAS, Scer\GAL4^elav-C155

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has visible | adult stage phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has abnormal neuroanatomy | adult stage phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Suppressed by

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype, suppressible | partially by Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype, suppressible | partially by Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has visible phenotype, suppressible by Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has visible phenotype, suppressible by Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, suppressible by Ide^UAS.cTa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, suppressible by Hsap\IDE^UAS.cTa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has partially lethal - majority die phenotype, suppressible | partially by Psn^C4/Psn[+]

(Greeve et al., 2004)

NOT Enhancer of

Statement

Reference

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 is a non-enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\APP^695.UAS.Exel, Scer\GAL4^elav-C155

(Sarantseva et al., 2009)

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 is a non-enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\APP^{695-Swedish.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Sarantseva et al., 2009)

Suppressor of

Statement

Reference

Hsap\BACE1^UAS.cGa is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\APP^{s.β.UAS.Tag:HA}, SNF4Aγ^loe, Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 is a suppressor | partially of short lived phenotype of Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 is a suppressor | partially of short lived phenotype of Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of visible phenotype of Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of visible phenotype of Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\BACE1^UAS.cGa, Scer\GAL4^GMR.PF is a suppressor | partially of increased cell death phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

Other

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has decreased rate of movement | adult stage phenotype

(Zhang et al., 2015)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^{elav.Switch.PO} has increased rate of movement | adult stage | RU486 conditional phenotype

(Zhang et al., 2015)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^{elav.Switch.PO} has increased rate of movement | adult stage phenotype

(Zhang et al., 2015)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal neuroanatomy | third instar larval stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has visible | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal neuroanatomy | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has melanotic mass phenotype | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal memory | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal size | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage | progressive phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype

(Chakraborty et al., 2011, Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has visible phenotype

(Tsuda et al., 2010)

Hsap\APP^695.UAS.Exel, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal learning | adult stage | progressive phenotype

(Sarantseva et al., 2009)

Hsap\APP^{695-Swedish.UAS.Tag:MYC}, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal memory | adult stage | progressive phenotype

(Sarantseva et al., 2009)

Hsap\APP^{695-Swedish.UAS.Tag:MYC}, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal learning | adult stage | progressive phenotype

(Sarantseva et al., 2009)

Hsap\APP^695.UAS.Exel, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has abnormal memory | adult stage | progressive phenotype

(Sarantseva et al., 2009)

Df(2R)Egfr5/+, Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has short lived phenotype

(Muhammad et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has wing vein phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act.PU has brain | adult stage phenotype, enhanceable by Df(2R)Egfr5/+

(Muhammad et al., 2008)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, enhanceable by Psn^+14.UAS, Scer\GAL4^Act5C.PI

(Greeve et al., 2004)

Suppressed by

Statement

Reference

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has eye photoreceptor cell phenotype, suppressible by Ide^UAS.cTa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has eye photoreceptor cell phenotype, suppressible by Hsap\IDE^UAS.cTa, Scer\GAL4^elav-C155

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, suppressible by Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, suppressible by Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype, suppressible | partially by Psn^C4/Psn[+]

(Greeve et al., 2004)

NOT Enhancer of

Statement

Reference

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 is a non-enhancer of adult brain | progressive phenotype of Hsap\APP^695.UAS.Exel, Scer\GAL4^elav-C155

(Sarantseva et al., 2009)

Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 is a non-enhancer of adult brain | progressive phenotype of Hsap\APP^{695-Swedish.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Sarantseva et al., 2009)

Suppressor of

Statement

Reference

Hsap\BACE1^UAS.cGa is a suppressor of larval brain | third instar larval stage phenotype of Hsap\APP^{s.β.UAS.Tag:HA}, SNF4Aγ^loe, Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

Ide^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of wing vein | ectopic phenotype of Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\IDE^UAS.cTa, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of wing vein | ectopic phenotype of Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI

(Tsuda et al., 2010)

Hsap\BACE1^UAS.cGa, Scer\GAL4^GMR.PF is a suppressor | partially of eye photoreceptor cell phenotype of Hsap\APP^695.UAS, Scer\GAL4^GMR.PF

(Greeve et al., 2004)

Other

Statement

Reference

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has type I bouton phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has wing | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has mushroom body | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^elav-C155 has proboscis | adult stage phenotype

(Mhatre et al., 2014)

Hsap\APP^695.UAS, Hsap\BACE1^UAS.cGa, Scer\GAL4^Act5C.PI has wing vein | ectopic phenotype

(Tsuda et al., 2010, Greeve et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa driven by Scer\GAL4^{elav.Switch.PO} driven by Scer\GAL4^{elav.Switch.PO} (with 200um RU486) results in significant hyperactivity (including nocturnal hyperactivity followed by a rapid decline, the effect is more prominent in male flies, and the effect disappears as flies age); this effect is more striking on a diet with a high carbohydrate to protein ratio (compared to low carbohydrate to protein ratio).

Co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa driven by Scer\GAL4^elav-C155 results in significant hypoactivity compared to controls, though total activity at night is not significantly altered (it is significantly decreased during the day).

(Zhang et al., 2015)

Larvae co-expressing Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 show a significant decrease in third instar larval body wall contractions.

The crawling defects seen when Hsap\BACE1^Scer\UAS.cGa is expressed under the control of Scer\GAL4^elav-C155 are enhanced upon co-expression of Hsap\APP^695.Scer\UAS.

Co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 results in structural changes in the synapse of the third instar larval muscle 6 and 7 neuromuscular junctions (NMJs). The overall number of boutons is reduced compared to controls, and this is due to a reduced number of type 1s boutons; the number of 1b boutons is similar to wild type. Both the number of motor neuron branches and the size of muscles 6 and 7 at the NMJ are similar to controls. The density of presynaptic release sites (brp-positive active zones) in motor neurons is also normal, but mitochondrial localisation defects are observed.

(Mhatre et al., 2014)

Expression of both Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 leads to a subset of flies showing melanotic masses on the ventral proboscis, curled wings, and these flies show a significant and progressive defect in climbing ability beginning at 42 days post-eclosion, a significant decrease in the size of the mushroom body, and a significant decrease in immediate recall memory in a conditioned courtship suppression assay, but do not show a significant decrease in lifespan nor any significant defect in learning ability as compared to controls.

(Mhatre et al., 2014)

Co-expression of Hsap\BACE1^Scer\UAS.cGa suppresses the increase in vacuolisation seen when Hsap\APP^{695.Scer\UAS.Exel} is expressed in the brains of SNF4Aγ^loe mutant third instar larvae under the control of Scer\GAL4^Appl.G1a.

(Wentzell et al., 2012)

The pan-neuronal co-expression of Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 leads to a decrease in adult lifespan, as compared to controls.

(Chakraborty et al., 2011)

Forced expression of Ide^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 can suppress the neuronal degeneration induced by the expression of Hsap\BACE1^Scer\UAS.cGa.

Forced expression of Hsap\IDE^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 can suppress the neuronal degeneration induced by the expression of Hsap\BACE1^Scer\UAS.cGa.

Co-expression of Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^elav-C155 results in reduced lifespan compared with controls.

The reduced lifespan of flies co-expressing Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^elav-C155 is partially suppressed by the co-expression of Ide^Scer\UAS.cTa.

The ectopic wing vein phenotype induced by co-expressing Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Act5C.PI is suppressed by the co-expression of Ide^Scer\UAS.cTa.

(Tsuda et al., 2010)

The loss of tissue due to age-progressive neurodegeneration (vacuolar lesions) observed in the brains of adult flies expressing either Hsap\APP^{695.Scer\UAS.Exel} or Hsap\APP^{695-Swedish.Scer\UAST:Hsap\MYC} under the control of Scer\GAL4^elav-C155 is not significantly affected by co-expression of Hsap\BACE1^Scer\UAS.cGa. Flies expressing either Hsap\APP^{695.Scer\UAS.Exel} or Hsap\APP^{695-Swedish.Scer\UAST:Hsap\MYC} alone or in combination with Hsap\BACE1^Scer\UAS.cGa display strong age-progressive defects.

(Sarantseva et al., 2009)

One copy of Df(2R)Egfr5 enhances the wing vein phenotype seen when Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa are co-expressed under the control of Scer\GAL4^Act.PU. The neurodegeneration is also increased, with at least 50% of flies showing clear evidence of neurodegeneration throughout the central brain compared to ~10% in controls and, unlike in controls, a significant fraction show dramatic loss of brain tissue.

(Muhammad et al., 2008)

The survival rates and wing patterns are not affected when flies coexpress Psn^+14.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI.

Coexpression of Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS, under the control of Scer\GAL4^GMR.PF, leads to a retinal degeneration phenotype that is less severe than that seen when Hsap\APP^695.Scer\UAS is expressed alone.

Flies that coexpress Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI exhibit ectopic wing veins. This phenotype is enhanced in flies that express Psn^+14.Scer\UAS in addition to Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI.

A Psn^C4/+ background partially rescues the ectopic wing vein formation and semi-lethality of flies that coexpress Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI.

(Greeve et al., 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\BACE1^Scer\UAS.cGa

Hsap\BACE1^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Greeve

Secondary FlyBase IDs

References (15)

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