Allele Hsap\BACE1^Scer\UAS.cGa

General Information
Symbol	Hsap\BACE1^Scer\UAS.cGa	Species	H. sapiens
Name	Saccharomyces cerevisiae UAS construct a of Greeve	FlyBase ID	FBal0213065
Feature type	allele	Associated gene	Hsap\BACE1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms neuroanatomy defective eye photoreceptor cell
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Greeve et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a 1.9kb KpnI-XhoI fragment of Hsap\BACE1, which contains the entire open reading frame. (Greeve et al., 2004)
Carried in construct	P{UAS-BACE1.G} (Greeve et al., 2004, Rajendran et al., 2008, Wentzell et al., 2012, Carmine-Simmen et al., 2009, Tsuda et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^elav-C155 (Tsuda et al., 2010)
Phenotype Manifest In
	eye photoreceptor cell, with Scer\GAL4^elav-C155 (Tsuda et al., 2010)
Detailed Description
	Statement Reference Expression of Hsap\BACE1[Scer\UAS.cGa] in photoreceptor neurons under the control of Scer\GAL4[elav-C155] results in the degeneration of the highly organised architecture of retinal photoreceptors in an age-dependent manner. (Tsuda et al., 2010) Expression of Hsap\BACE1^Scer\UAS.cGa in eyes under the control of Scer\GAL4^GMR.PF does not cause retinal degeneration. (Greeve et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has partially lethal - majority die phenotype, suppressible \| partially by Psn^C4/Psn[+] (Greeve et al., 2004) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has visible phenotype, suppressible by Hsap\IDE^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has visible phenotype, suppressible by Ide^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype, suppressible \| partially by Hsap\IDE^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 (Tsuda et al., 2010, Tsuda et al., 2010) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype, suppressible \| partially by Ide^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 (Tsuda et al., 2010, Tsuda et al., 2010) Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 has neuroanatomy defective phenotype, suppressible by Hsap\IDE^Scer\UAS.cTa, Scer\GAL4^elav-C155 (Tsuda et al., 2010) Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 has neuroanatomy defective phenotype, suppressible by Ide^Scer\UAS.cTa, Scer\GAL4^elav-C155 (Tsuda et al., 2010)
Suppressor of
Statement Reference Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor \| partially of increased cell death phenotype of Hsap\APP^695.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Greeve et al., 2004) Hsap\BACE1^Scer\UAS.cGa is a suppressor of neuroanatomy defective \| third instar larval stage phenotype of Hsap\APP^{s.β.Scer\UAS.T:Ivir\HA1}, SNF4Aγ^loe, Scer\GAL4^Appl.G1a (Wentzell et al., 2012) Hsap\IDE^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of visible phenotype of Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010) Hsap\IDE^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 is a suppressor \| partially of short lived phenotype of Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 (Tsuda et al., 2010, Tsuda et al., 2010) Ide^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of visible phenotype of Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010) Ide^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 is a suppressor \| partially of short lived phenotype of Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 (Tsuda et al., 2010, Tsuda et al., 2010)
Other
Statement Reference Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI has visible phenotype (Tsuda et al., 2010) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 has short lived phenotype (Tsuda et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has wing vein \| ectopic phenotype, enhanceable by Psn^+14.Scer\UAS, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Greeve et al., 2004)
Suppressed by
Statement Reference Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has wing vein \| ectopic phenotype, suppressible \| partially by Psn^C4/Psn[+] (Greeve et al., 2004) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has wing vein \| ectopic phenotype, suppressible by Hsap\IDE^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has wing vein \| ectopic phenotype, suppressible by Ide^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010) Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 has eye photoreceptor cell phenotype, suppressible by Hsap\IDE^Scer\UAS.cTa, Scer\GAL4^elav-C155 (Tsuda et al., 2010) Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^elav-C155 has eye photoreceptor cell phenotype, suppressible by Ide^Scer\UAS.cTa, Scer\GAL4^elav-C155 (Tsuda et al., 2010)
Suppressor of
Statement Reference Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor \| partially of eye photoreceptor cell phenotype of Hsap\APP^695.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Greeve et al., 2004) Hsap\BACE1^Scer\UAS.cGa is a suppressor of embryonic/larval brain \| third instar larval stage phenotype of Hsap\APP^{s.β.Scer\UAS.T:Ivir\HA1}, SNF4Aγ^loe, Scer\GAL4^Appl.G1a (Wentzell et al., 2012) Hsap\IDE^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of wing vein \| ectopic phenotype of Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010) Ide^Scer\UAS.cTa, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI is a suppressor of wing vein \| ectopic phenotype of Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Tsuda et al., 2010, Tsuda et al., 2010)
Other
Statement Reference Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has wing vein \| ectopic phenotype (Greeve et al., 2004) Hsap\APP^695.Scer\UAS, Hsap\BACE1^Scer\UAS.cGa, Scer\GAL4^Act5C.PI has wing vein \| ectopic phenotype (Tsuda et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Hsap\BACE1[Scer\UAS.cGa] suppresses the increase in vacuolisation seen when Hsap\APP[695.Scer\UAS.Exel] is expressed in the brains of SNF4Aγ[loe] mutant third instar larvae under the control of Scer\GAL4[Appl.G1a]. (Wentzell et al., 2012) Forced expression of Ide[Scer\UAS.cTa] under the control of Scer\GAL4[elav-C155] can suppress the neuronal degeneration induced by the expression of Hsap\BACE1[Scer\UAS.cGa]. Forced expression of Hsap\IDE[Scer\UAS.cTa] under the control of Scer\GAL4[elav-C155] can suppress the neuronal degeneration induced by the expression of Hsap\BACE1[Scer\UAS.cGa]. Co-expression of Hsap\BACE1[Scer\UAS.cGa] and Hsap\APP[695.Scer\UAS] under the control of Scer\GAL4[elav-C155] results in reduced lifespan compared with controls. The reduced lifespan of flies co-expressing Hsap\BACE1[Scer\UAS.cGa] and Hsap\APP[695.Scer\UAS] under the control of Scer\GAL4[elav-C155] is partially suppressed by the co-expression of Ide[Scer\UAS.cTa]. The reduced lifespan of flies co-expressing Hsap\BACE1[Scer\UAS.cGa] and Hsap\APP[695.Scer\UAS] under the control of Scer\GAL4[elav-C155] is partially suppressed by the co-expression of Hsap\IDE[Scer\UAS.cTa]. The ectopic wing vein phenotype induced by co-expressing Hsap\BACE1[Scer\UAS.cGa] and Hsap\APP[695.Scer\UAS] under the control of Scer\GAL4[Act5C.PI] is suppressed by the co-expression of Ide[Scer\UAS.cTa]. The ectopic wing vein phenotype induced by co-expressing Hsap\BACE1[Scer\UAS.cGa] and Hsap\APP[695.Scer\UAS] under the control of Scer\GAL4[Act5C.PI] is suppressed by the co-expression of Hsap\IDE[Scer\UAS.cTa]. (Tsuda et al., 2010) The survival rates and wing patterns are not affected when flies coexpress Psn^+14.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI. Coexpression of Hsap\BACE1^Scer\UAS.cGa and Hsap\APP^695.Scer\UAS, under the control of Scer\GAL4^GMR.PF, leads to a retinal degeneration phenotype that is less severe than that seen when Hsap\APP^695.Scer\UAS is expressed alone. Flies that coexpress Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI exhibit ectopic wing veins. This phenotype is enhanced in flies that express Psn^+14.Scer\UAS in addition to Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI. A Psn^C4/+ background partially rescues the ectopic wing vein formation and semi-lethality of flies that coexpress Hsap\APP^695.Scer\UAS and Hsap\BACE1^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI. (Greeve et al., 2004)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Hsap\BACE1^Scer\UAS.cGa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Greeve
Secondary FlyBase IDs

References ( 5 )
Research paper	Wentzell et al., 2012, Neurobiol. Disease 46(1): 78--87 Amyloid precursor proteins are protective in Drosophila models of progressive neurodegeneration. [FBrf0217742] Tsuda et al., 2010, FEBS Lett. 584(13): 2916--2920 Insulin-degrading enzyme antagonizes insulin-dependent tissue growth and Abeta-induced neurotoxicity in Drosophila. [FBrf0210992] Carmine-Simmen et al., 2009, Neurobiol. Disease 33(2): 274--281 Neurotoxic effects induced by the Drosophila amyloid-beta peptide suggest a conserved toxic function. [FBrf0206813] Rajendran et al., 2008, Science 320(5875): 520--523 Efficient inhibition of the Alzheimer's disease beta-secretase by membrane targeting. [FBrf0204630] Greeve et al., 2004, J. Neurosci. 24(16): 3899--3906 Age-dependent neurodegeneration and Alzheimer-amyloid plaque formation in transgenic Drosophila. [FBrf0174857]

Allele Hsap\BACE1Scer\UAS.cGa

Allele Hsap\BACE1^Scer\UAS.cGa