Allele Dmel\ds^{ecto.Scer\UAS}

General Information
Symbol	Dmel\ds^{ecto.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0213079
Feature type	allele	Associated gene	Dmel\ds

Allele class
Mutagen	in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Casal et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	ds^Scer\UAS.cTa (Casal et al., 2006)
Nature of the lesion	Statement Reference Deletion of the ds cytosolic domain. (Casal et al., 2006)
Carried in construct	P{UAS-ds.ecto} (Casal et al., 2006, Brittle et al., 2010, Repiso et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	planar polarity defective \| cell non-autonomous \| somatic clone, with Scer\GAL4^αTub84B.PL (Casal et al., 2006) planar polarity defective \| heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B (Repiso et al., 2010)
Phenotype Manifest In
	denticle belt \| heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B (Repiso et al., 2010) trichome & abdominal tergite \| somatic clone \| cell non-autonomous, with Scer\GAL4^αTub84B.PL (Casal et al., 2006)
Detailed Description
	Statement Reference Expression of ds[ecto.Scer\UAS] under the control of Scer\GAL4[ptc-559.1] and Scer\GAL80[ts.αTub84B] at 17[o]C has no effect on denticle belt polarity. However, growth at 29[o]C results in severe denticle polarity defects. Defects are also seen when the temperature is shifted from 17[o]C to 29[o]C before the moult to second stage larva. The converse shift, from 29[o]C to 17[o]C at the second instar larval stage results in wild-type denticle belt polarity. (Repiso et al., 2010) Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a wild-type background usually result in a reversal of cell polarity in wild-type cells posterior to the clone (hairs behind the clone point forwards), with a range of several cells. Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a ds^unspecified background do not change the cell polarity of cells surrounding the clone. Clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are mutant for ds^unspecified and are also expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL can reverse the cell polarity of cells posterior to the clone. (Casal et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^αTub84B.PL, ds^UAO71, ds^{ecto.Scer\UAS}, ft¹⁵ has planar polarity defective \| somatic clone \| cell non-autonomous phenotype, suppressible \| somatic clone by fj^Scer\UAS.cZa/Scer\GAL4^αTub84B.PL (Brittle et al., 2010)
Other
Statement Reference Scer\GAL4^αTub84B.PL, ds^UAO71, ds^{ecto.Scer\UAS}, ft¹⁵ has planar polarity defective \| somatic clone \| cell non-autonomous phenotype (Brittle et al., 2010)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^αTub84B.PL, ds^UAO71, ds^{ecto.Scer\UAS}, ft¹⁵ has trichome & tergite \| cell non-autonomous \| somatic clone phenotype, suppressible \| somatic clone by fj^Scer\UAS.cZa/Scer\GAL4^αTub84B.PL (Brittle et al., 2010)
Other
Statement Reference Scer\GAL4^αTub84B.PL, ds^UAO71, ds^{ecto.Scer\UAS}, ft¹⁵ has trichome & tergite \| somatic clone \| cell non-autonomous phenotype (Brittle et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference ds[UAO71] ft[15] double mutant clones in the dorsal adult abdomen where the clones are also expressing ds[ecto.Scer\UAS] under the control of Scer\GAL4[αTub84B.PL] strongly repolarise hairs behind the clone, reversing their polarity (100% of clones repolarise with a maximum range of up to seven cells). This repolarisation is largely suppressed by co-expression of fj[Scer\UAS.cZa] within the clone (48% of clones show some repolarisation with a range of one to three cells). (Brittle et al., 2010) The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59). The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a fz^unspecified background. The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a fz^unspecified stan^- background (cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59). Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a ft¹²/ft^unspecified background do not change the cell polarity of cells surrounding the clone. Clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are mutant for ft^unspecified and are also expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL can reverse the cell polarity of cells posterior to the clone. Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both fj^Scer\UAS.cZa and ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone. Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both ft^Scer\UAS.cMa and ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone. The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is enhanced if the clones are induced in a fj^unspecified background compared to a wild-type background; the repolarisation occurs over a greater range in the fj^unspecified background. (Casal et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ds^{ecto.Scer\UAS} (Brittle et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Brittle et al., 2010, Curr. Biol. 20(9): 803--810 Four-jointed modulates growth and planar polarity by reducing the affinity of dachsous for fat. [FBrf0210769] Repiso et al., 2010, Development 137(20): 3411--3415 Planar cell polarity: the orientation of larval denticles in Drosophila appears to depend on gradients of Dachsous and Fat. [FBrf0211889] Casal et al., 2006, Development 133(22): 4561--4572 Two separate molecular systems, Dachsous/Fat and Starry night/Frizzled, act independently to confer planar cell polarity. [FBrf0194266]

Allele Dmel\dsecto.Scer\UAS

Allele Dmel\ds^{ecto.Scer\UAS}