Open Close
General Information
Symbol
Dmel\MlfΔ5-3
Species
D. melanogaster
Name
FlyBase ID
FBal0213087
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference
Imprecise excision of the P{EP} insertion in MlfEU2490, resulting in a 2kb deletion removing nucleotides -356 to +1716 of Mlf (coordinates relative to the transcription start site of isoforms A,B and D).
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Crystal cell maintenance is impaired in both MlfΔ5-3/MlfΔC1 and homozygous MlfΔ5-3 embryos resulting in a progressive decrease in crystal cell numbers. In contrast, the number of crystal cells in homozygous MlfΔ5-3 third instar larvae is increased compared with wild-type.
83.3% of animals derived from a cross of homozygous females to homozygous males die as embryos, whereas 64.4% of animals derived from a cross of homozygous females to wild-type males die, indicating that that maternal effect lethality is partially rescued by a paternal Mlf+ copy. Embryos derived from a cross of heterozygous females to wild-type males have reduced viability (38.7% of these animals die as embryos) compared to controls. 90.0% of animals derived from a cross of homozygous MlfΔ5-3 females to homozygous MlfΔC1 males die as embryos. Homozygous adults lack interocellar bristles and the head macrochaetae are frequently bent or shortened. Ectopic venation is seen in the wing near longitudinal vein 2.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
NOT Suppressor of
Statement
Reference
MlfΔ5-3/Mlf[+] is a non-suppressor of short lived | RU486 conditional phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4elav.Switch.PO
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Expression of lzScer\UAS.cBa under the control of Scer\GAL4lz-gal4 suppresses not only the drop in crystal cell number in MlfΔ5-3 mutant embryos, but also the rise in crystal cell number in MlfΔ5-3 larvae.
Xenogenetic Interactions
Statement
Reference
Expression of Hsap\MLF1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4lz-gal4 suppresses the hematopoietic defects in homozygous MlfΔ5-3 embryos.
The reduction in life span that is seen in flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 is not modified by the addition of MlfΔ5-3/+.
Complementation and Rescue Data
Comments
Expression of MlfScer\UAS.cFa under the control of Scer\GAL4lz-gal4 rescues the crystal cell phenotype of both MlfΔ5-3/MlfΔC1 and homozygous MlfΔ5-3 mutants.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)