Crystal cell maintenance is impaired in both MlfΔ5-3
and homozygous MlfΔ5-3
embryos resulting in a progressive decrease in crystal cell numbers. In contrast, the number of crystal cells in homozygous MlfΔ5-3
third instar larvae is increased compared with wild-type.
83.3% of animals derived from a cross of homozygous females to homozygous males die as embryos, whereas 64.4% of animals derived from a cross of homozygous females to wild-type males die, indicating that that maternal effect lethality is partially rescued by a paternal Mlf+
copy. Embryos derived from a cross of heterozygous females to wild-type males have reduced viability (38.7% of these animals die as embryos) compared to controls.
90.0% of animals derived from a cross of homozygous MlfΔ5-3
females to homozygous MlfΔC1
males die as embryos.
Homozygous adults lack interocellar bristles and the head macrochaetae are frequently bent or shortened. Ectopic venation is seen in the wing near longitudinal vein 2.