FlyBase Allele Report: Dmel\Mlf[Δ5-3]

General Information

Symbol

Dmel\Mlf^Δ5-3

Species

D. melanogaster

Name

FlyBase ID

FBal0213087

Feature type

allele

Associated gene

Dmel\Mlf

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Martin-Lanneree et al., 2006)

Progenitor genotype

Mlf^EU2490

(Martin-Lanneree et al., 2006)

Cytology

Description

Imprecise excision of the P{EP} insertion in Mlf^EU2490, resulting in a 2kb deletion removing nucleotides -356 to +1716 of Mlf (coordinates relative to the transcription start site of isoforms A,B and D).

(Martin-Lanneree et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | rescuable maternal effect

(Martin-Lanneree et al., 2006)

some die during embryonic stage | rescuable maternal effect

(Martin-Lanneree et al., 2006)

visible

(Martin-Lanneree et al., 2006)

Phenotype Manifest In

adult head bristle

(Martin-Lanneree et al., 2006)

embryonic/larval crystal cell | embryonic stage 15

(Bras et al., 2012)

embryonic/larval crystal cell | embryonic stage 15 (with Mlf^ΔC1)

(Bras et al., 2012)

embryonic/larval crystal cell | third instar larval stage

(Bras et al., 2012)

interocellar bristle

(Martin-Lanneree et al., 2006)

wing vein | ectopic

(Martin-Lanneree et al., 2006)

Detailed Description

Statement

Reference

Crystal cell maintenance is impaired in both Mlf^Δ5-3/Mlf^ΔC1 and homozygous Mlf^Δ5-3 embryos resulting in a progressive decrease in crystal cell numbers. In contrast, the number of crystal cells in homozygous Mlf^Δ5-3 third instar larvae is increased compared with wild-type.

(Bras et al., 2012)

83.3% of animals derived from a cross of homozygous females to homozygous males die as embryos, whereas 64.4% of animals derived from a cross of homozygous females to wild-type males die, indicating that that maternal effect lethality is partially rescued by a paternal Mlf⁺ copy. Embryos derived from a cross of heterozygous females to wild-type males have reduced viability (38.7% of these animals die as embryos) compared to controls.

90.0% of animals derived from a cross of homozygous Mlf^Δ5-3 females to homozygous Mlf^ΔC1 males die as embryos.

Homozygous adults lack interocellar bristles and the head macrochaetae are frequently bent or shortened. Ectopic venation is seen in the wing near longitudinal vein 2.

(Martin-Lanneree et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Mlf^Δ5-3 is a suppressor of hyperplasia | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4

(Bras et al., 2012)

NOT Suppressor of

Statement

Reference

Mlf^Δ5-3/Mlf[+] is a non-suppressor of short lived | RU486 conditional phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^{elav.Switch.PO}

(Martin-Lanneree et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mlf^Δ5-3 has embryonic/larval crystal cell | embryonic stage 15 phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Bras et al., 2012)

Mlf^Δ5-3 has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Bras et al., 2012)

Mlf^Δ5-3 has embryonic/larval crystal cell | embryonic stage 15 phenotype, suppressible by Hsap\MLF1^UAS.Tag:FLAG/Scer\GAL4^lz-gal4

(Bras et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of lz^Scer\UAS.cBa under the control of Scer\GAL4^lz-gal4 suppresses not only the drop in crystal cell number in Mlf^Δ5-3 mutant embryos, but also the rise in crystal cell number in Mlf^Δ5-3 larvae.

(Bras et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\MLF1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^lz-gal4 suppresses the hematopoietic defects in homozygous Mlf^Δ5-3 embryos.

(Bras et al., 2012)

The reduction in life span that is seen in flies expressing Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 is not modified by the addition of Mlf^Δ5-3/+.

(Martin-Lanneree et al., 2006)

Complementation and Rescue Data

Rescued by

Mlf^Δ5-3 is rescued by Mlf^UAS.cFa

(Bras et al., 2012)

Mlf^Δ5-3/Mlf^ΔC1 is rescued by Mlf^UAS.cFa

(Bras et al., 2012)

Comments

Expression of Mlf^Scer\UAS.cFa under the control of Scer\GAL4^lz-gal4 rescues the crystal cell phenotype of both Mlf^Δ5-3/Mlf^ΔC1 and homozygous Mlf^Δ5-3 mutants.

(Bras et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mlf^Δ5-3

dmlf^Δ5-3

(Martin-Lanneree et al., 2006)

mlf^Δ5-3

(Bras et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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