83.3% of animals derived from a cross of homozygous females to homozygous males die as embryos, whereas 64.4% of animals derived from a cross of homozygous females to wild-type males die, indicating that that maternal effect lethality is partially rescued by a paternal Mlf+ copy. Embryos derived from a cross of heterozygous females to wild-type males have reduced viability (38.7% of these animals die as embryos) compared to controls.
90.0% of animals derived from a cross of homozygous MlfΔ5-3 females to homozygous MlfΔC1 males die as embryos.
Homozygous adults lack interocellar bristles and the head macrochaetae are frequently bent or shortened. Ectopic venation is seen in the wing near longitudinal vein 2.
MlfΔ5-3 is a suppressor of hyperplasia | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1UAS.cMa, Scer\GAL4lz-gal4
MlfΔ5-3/Mlf[+] is a non-suppressor of short lived | RU486 conditional phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4elav.Switch.PO
MlfΔ5-3 has embryonic/larval crystal cell | embryonic stage 15 phenotype, suppressible by lzUAS.cBa/Scer\GAL4lz-gal4
MlfΔ5-3 has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible by lzUAS.cBa/Scer\GAL4lz-gal4
MlfΔ5-3 has embryonic/larval crystal cell | embryonic stage 15 phenotype, suppressible by Hsap\MLF1UAS.Tag:FLAG/Scer\GAL4lz-gal4
Expression of lzScer\UAS.cBa under the control of Scer\GAL4lz-gal4 suppresses not only the drop in crystal cell number in MlfΔ5-3 mutant embryos, but also the rise in crystal cell number in MlfΔ5-3 larvae.
Expression of Hsap\MLF1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4lz-gal4 suppresses the hematopoietic defects in homozygous MlfΔ5-3 embryos.
The reduction in life span that is seen in flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 is not modified by the addition of MlfΔ5-3/+.
MlfΔ5-3 is rescued by MlfUAS.cFa
MlfΔ5-3/MlfΔC1 is rescued by MlfUAS.cFa
Expression of MlfScer\UAS.cFa under the control of Scer\GAL4lz-gal4 rescues the crystal cell phenotype of both MlfΔ5-3/MlfΔC1 and homozygous MlfΔ5-3 mutants.