Allele Dmel\Apc2³³

General Information
Symbol	Dmel\Apc2³³	Species	D. melanogaster
Name		FlyBase ID	FBal0213105
Feature type	allele	Associated gene	Dmel\Apc2

Allele class	hypomorphic allele - genetic evidence
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Takacs et al., 2008)
Mutagen	Delta2-3 (Takacs et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EPgy2}EY09246 (Takacs et al., 2008)
Nature of the lesion	Statement Reference Imprecise excision of the P{EPgy2}EY09246 insertion, resulting in a deletion of nucleotides -431 to +1307 relative to the Apc2 transcription start site. 720bp of the insertion remain at this deletion site. There is a second deletion of 4bp at positions +1313 to +1316. (Takacs et al., 2008)
Caused by insertion	P{?EPgy2}Apc2³³ (Takacs et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	denticle belt (Takacs et al., 2008) embryonic/first instar larval cuticle (Takacs et al., 2008) embryonic/larval cuticle \| embryonic stage (Roberts et al., 2012)
Detailed Description
	Statement Reference Embryos maternally and zygotically mutant for Apc2[33] have an average cuticle score of 3.2 (based on the numerical scale described in McCartney et al., 1994, where 0 is a wild type embryo and 6 indicates the most severe defects). (Roberts et al., 2012) Severe hypomorph. Homozygous embryos derived from homozygous females show an expansion of naked cuticle at the expense of denticles. (Takacs et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Apc2³³, Apc^Q8, Axn^S044230 has neuroanatomy defective \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012) Apc2³³, Apc^Q8 has neuroanatomy defective \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012) Apc2³³/Apc2^5-3, Apc^Q8 has visible phenotype (Takacs et al., 2008) Apc2^G5028/Apc2³³, Apc^Q8 has visible phenotype (Takacs et al., 2008)
Phenotype Manifest In
Suppressed by
Statement Reference Apc2³³, Apc^Q8 has intestinal stem cell \| somatic clone phenotype, suppressible by pan^ΔN.Scer\UAS/Scer\GAL4^tub.PU (Lee et al., 2009)
Suppressor of
Statement Reference Apc2[+]/Apc2³³ is a suppressor of eye photoreceptor cell phenotype of Apc^Q8 (Takacs et al., 2008) Apc2³³/Apc2³³ is a suppressor of eye photoreceptor cell phenotype of Scer\GAL4^elav-C155, arm^{Scer\UAS.Exel} (Takacs et al., 2008, Takacs et al., 2008) Apc2³³ is a suppressor of eye photoreceptor cell phenotype of Apc^Q8 (Takacs et al., 2008)
Other
Statement Reference Apc2³³, Apc^Q8, Axn^S044230 has medulla \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012) Apc2³³, Apc^Q8 has intestinal stem cell \| somatic clone phenotype (Lee et al., 2009) Apc2³³, Apc^Q8 has medulla \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012) Apc2³³/Apc2^5-3, Apc^Q8 has abdominal sternite bristle \| ectopic phenotype (Takacs et al., 2008) Apc2³³/Apc2^5-3, Apc^Q8 has pleural membrane phenotype (Takacs et al., 2008) Apc2³³/Apc2^5-3, Apc^Q8 has sternite phenotype (Takacs et al., 2008) Apc2^G5028/Apc2³³, Apc^Q8 has abdominal sternite bristle \| ectopic phenotype (Takacs et al., 2008) Apc2^G5028/Apc2³³, Apc^Q8 has pleural membrane phenotype (Takacs et al., 2008) Apc2^G5028/Apc2³³, Apc^Q8 has sternite phenotype (Takacs et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference No apical constriction is seen in the cells surrounding the wing pouch in Apc[Q8] Apc2[33] double mutant third instar larval clones. Apc2[19-3] Apc[Q8] double mutant clone cells generated in the medullar region of third instar larval brains sometimes segregate from their neighbours to form cysts. When clones are induced in the medullar neurons the axons extend correctly to the medullar neuropil. (Roberts et al., 2012) Apc2[33] Apc[Q8] double mutant intestinal stem cell clones in the adult midgut show hyperplasia. The hyperplasia seen in Apc2[33] Apc[Q8] double mutant intestinal stem cell clones in the adult midgut is completely suppressed if they are also expressing pan[ΔN.Scer\UAS] under the control of Scer\GAL4[tub]. (Lee et al., 2009) The photoreceptor cell apoptosis phenotype seen in Apc^Q8 homozygotes is suppressed by Apc2³³/+. (Takacs et al., 2008) The photoreceptor apoptosis phenotype seen in flies expressing arm^{Scer\UAS.Exel} under the control of Scer\GAL4^elav-C155 is partially suppressed by Apc2³³/Apc2³³. Apc^Q8 Apc2^5-3/Apc^Q8 Apc2³³ double mutants show expansion of sternal territory in the abdomen and ectopic sternal bristles, at the expense of the pleura. Apc^Q8 Apc2^G5028/Apc^Q8 Apc2³³ double mutants show expansion of sternal territory in the abdomen and ectopic sternal bristles, at the expense of the pleura. (Takacs et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Apc2³³ (Takacs et al., 2008, Benchabane et al., 2011, Takacs et al., 2008, Lee et al., 2009) APC2³³ (Roberts et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Roberts et al., 2012, PLoS ONE 7(2): e31284 Defining Components of the ßcatenin Destruction Complex and Exploring Its Regulation and Mechanisms of Action during Development. [FBrf0217546] Lee et al., 2009, Development 136(13): 2255--2264 Adenomatous polyposis coli regulates Drosophila intestinal stem cell proliferation. [FBrf0208211] Takacs et al., 2008, Science 319(5861): 333--336 Dual positive and negative regulation of wingless signaling by adenomatous polyposis coli. [FBrf0202792]
Supplementary material	Benchabane et al., 2011, EMBO J. 30(8): Supplementary Information. [FBrf0213708] Takacs et al., 2008, Science 319(5861): Supporting online material. [FBrf0202572]

Allele Dmel\Apc233

Allele Dmel\Apc2³³