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General Information
Symbol
Dmel\CDaseslab-1
Species
D. melanogaster
Name
FlyBase ID
FBal0213206
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G30463751A

Amino acid change:

V264M | CDase-PA; V264M | CDase-PB; V264M | CDase-PC; V264M | CDase-PD; V264M | CDase-PE; V264M | CDase-PF

Reported amino acid change:

V264M

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: V264M.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

CDaseslab-1 embryos appear anatomically and morphologically normal. Their neuromuscular junctions appear morphologically normal. Although mutants move spontaneously and in response before hatching, movements are less vigorous than in controls. A minority briefly generate peristaltic body contractions sufficient to emerge partly or completely from the egg case. In contrast, CDaseslab-1/+ siblings display wild-type movement and behavior.

Electrophysiological assays performed at late embryonic stages or early larval stages reveal that CDaseslab-1 mutants show impaired neurotransmission due to a presynaptic defect. Nerve-evoked EJC amplitude and fidelity are reduced compared to controls and show transmission failures and near-failures. The incidence of failures progressively increases at higher stimulation frequencies. However, postsynaptic glutamate-gated currents are normal. Additionally, hypertonic saline-evoked synaptic vesicle fusion is significantly impaired in CDaseslab-1, CDaseslab-1/CDaseslab-2 and CDaseslab-1/Df(3R)slab-3 mutants.

FM1-43 dye uptake is reduced in CDaseslab-1 presynaptic terminals, indicating a smaller cycling vesicle pool. Ultrastructural analyses of CDaseslab-1/Df(3R)slab-3 mutants reveal that the mutant presynaptic terminals have normal clustered and docked readily-releasable pools of vesicles. However, CDaseslab-1 boutons have fewer synaptic vesicles within the nonclustered population removed from the active zone and the boutons contain increased numbers of synaptic vesicles linked together in multi-vesicle clusters or chains. Furthermore, the boutons exhibit increased numbers of vesicles tethered by filamentous structures to the plasma membrane.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CDaseslab-1
Name Synonyms
Secondary FlyBase IDs
    References (1)