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General Information
Symbol
Dmel\BEAF-32AB-KO
Species
D. melanogaster
Name
FlyBase ID
FBal0215222
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
BEAFAB-KO
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
The duplication of BEAF-32 present in BEAF-32A-KO has been reduced to a single copy which contains mutations that knock out both isoform A and isoform B function. One mutation eliminates the ATG codon of the BEAF-32A isoform and destroys an NsiI site. A second mutation eliminates the ATG codon of the BEAF-32B isoform and creates an ApaI site. A third mutation introduces two tandem stop codons into the exon shared by both A and B isoforms and destroys a BamHI site.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Homozygous BEAF-32AB-KO mutants are viable and do not have defects in pupal interommatidial cell number; these, BEAF-32AB-KO/Df(2R)BSC429 animals and homozygous BEAF-32AB-KO retinal clones (covering either the entire retina or a portion of the retina) have an increased proportion of pale R8 photoreceptors and a reciprocally decreased proportion of yellow R8 photoreceptors, compared to controls. Homozygous BEAF-32AB-KO mutant clones (that cover a portion of the retina) do not have an altered ratio of pale to yellow R7 photoreceptors compared to control clones.
Homozygotes cannot be maintained as a stock. They are weaker than their heterozygous siblings and die within a few days if they are not transferred to a new vial containing a limited number of flies. Homozygous males have normal fertility when crossed to wild-type females and can live at least 2 weeks if pampered. Homozygous females have reduced fertility and the degree of fertility appears to be very sensitive to environmental conditions. Homozygous females mated to either homozygous or wild-type males lay fewer eggs than wild-type females, and the larval hatching rate of the progeny is approximately 40%. A higher proportion of progeny survive to pupal and adult stages if they are derived from the cross with wild-type (rather than homozygous) males, and the surviving adult progeny of this cross appear normal, are fertile and roughly equal numbers of female and male progeny are obtained. Homozygous females (that have been mated with wild-type males for 4 days) have a normal number of ovarioles per ovary, but the mutant ovaries are smaller than wild-type, because most ovarioles lack mature oocytes. The mutant germaria appear normal. Oogenesis defects become apparent at around stage 8 or later. Some egg chambers have too many nurse cell nuclei (this could be due to the fusion of two egg chambers or to an extra round of cell division). Other egg chambers have small nuclei that are probably an intermediate step in egg chamber degeneration. Homozygous flies have a rough eye phenotype. The polytene X chromosome from the salivary glands of homozygous male larvae shows obvious structural defects; the banding pattern is lost and the chromosome appears shorter and puffier than normal.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Addition of BEAF-32AB-KO/BEAF-32AB-KO to flies with sstransgene6 or sevunspecified suppresses the increased proportion of yellow R8 photoreceptors to the extent that the proportion is reduced compared to controls. In the case of sevunspecified, BEAF-32AB-KO/BEAF-32AB-KO reduces the proportion of yellow R8 photoreceptors to a similar level to that seen in BEAF-32AB-KO/BEAF-32AB-KO animals without sevunspecified. BEAF-32AB-KO/BEAF-32AB-KO retinal clones have suppression of the increased proportion of yellow R8 photoreceptors in flies with savGMR.PT and wtsGMR.PT, to the extent that the proportion is reduced compared to wild-type controls. sd12/sd12 retinal clones have suppression of the increased proportion of pale R8 photoreceptors in flies with BEAF-32AB-KO/Df(2R)BSC429, to the extent that the proportion is reduced compared to wild-type controls.
Homozygosity for BEAF-32AB-KO significantly increases the eye colour in flies carrying wP1\loxP.ME.RW+. Homozygosity for BEAF-32AB-KO has no effect on the eye colour of flies carrying w-ME.RW+.
Eye colour: flies carrying wMM.scs'.scs.cCa have darker pigmentation in the eyes if they are also homozygous for BEAF-32AB-KO (in 3/4 of P{wMM.scs'.scs.cCa} insertion lines tested). Body colour: yP1\loxP.2scs'-2scs' flies have darker pigmentation in the abdomen if they are also homozygous for BEAF-32AB-KO. Body colour: the level of pigmentation in the abdomen of yP1\loxP.gyp-gyp or yP1\loxP.scs-2scs' flies is unaffected if they are also homozygous for BEAF-32AB-KO. Eye colour: the eye pigmentation level of wa and we flies is not affected by BEAF-32AB-KO.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)