mutants are viable and do not have defects in pupal interommatidial cell number; these, BEAF-32AB-KO
animals and homozygous BEAF-32AB-KO
retinal clones (covering either the entire retina or a portion of the retina) have an increased proportion of pale R8 photoreceptors and a reciprocally decreased proportion of yellow R8 photoreceptors, compared to controls. Homozygous BEAF-32AB-KO
mutant clones (that cover a portion of the retina) do not have an altered ratio of pale to yellow R7 photoreceptors compared to control clones.
Homozygotes cannot be maintained as a stock. They are weaker than their heterozygous siblings and die within a few days if they are not transferred to a new vial containing a limited number of flies. Homozygous males have normal fertility when crossed to wild-type females and can live at least 2 weeks if pampered.
Homozygous females have reduced fertility and the degree of fertility appears to be very sensitive to environmental conditions. Homozygous females mated to either homozygous or wild-type males lay fewer eggs than wild-type females, and the larval hatching rate of the progeny is approximately 40%. A higher proportion of progeny survive to pupal and adult stages if they are derived from the cross with wild-type (rather than homozygous) males, and the surviving adult progeny of this cross appear normal, are fertile and roughly equal numbers of female and male progeny are obtained.
Homozygous females (that have been mated with wild-type males for 4 days) have a normal number of ovarioles per ovary, but the mutant ovaries are smaller than wild-type, because most ovarioles lack mature oocytes. The mutant germaria appear normal. Oogenesis defects become apparent at around stage 8 or later. Some egg chambers have too many nurse cell nuclei (this could be due to the fusion of two egg chambers or to an extra round of cell division). Other egg chambers have small nuclei that are probably an intermediate step in egg chamber degeneration.
Homozygous flies have a rough eye phenotype.
The polytene X chromosome from the salivary glands of homozygous male larvae shows obvious structural defects; the banding pattern is lost and the chromosome appears shorter and puffier than normal.