FlyBase Allele Report: Dmel\ttm50[UAS.Tag:HA]

General Information

Symbol

Dmel\ttm50^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0215237

Feature type

allele

Associated gene

Dmel\ttm50

Associated Insertion(s)

Carried in Construct

P{UAS-ttm50.HA}

Key Links

Transgenic product class

wild_type

(Sugiyama et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sugiyama et al., 2007)

Mutagen

in vitro construct

(Sugiyama et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-ttm50.HA}

Cytology

Description

UASt regulatory sequences drive expression of ttm50 tagged at the C-terminal end with a triple Tag:HA tag.

(Sugiyama et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ttm50

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaA | third instar larval stage, with Scer\GAL4^smid-C161

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4²²¹

(Tsuyama et al., 2017)

dendrite | pupal stage, with Scer\GAL4^ppk.PG

(Metwally et al., 2021)

dendrite | third instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with Scer\GAL4²²¹

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with Scer\GAL4^smid-C161

(Tsuyama et al., 2017)

eye, with Scer\GAL4^GMR.PF

(Sugiyama et al., 2007)

larval multidendritic class IV neuron | pupal stage, with Scer\GAL4^ppk.PG

(Metwally et al., 2021)

mitochondrion | third instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Detailed Description

Statement

Reference

The expression of ttm50^UAS.Tag:HA under the control of Scer\GAL4^ppk.PG results in longer dendrites in class IV da neurons at 7h APF.

(Metwally et al., 2021)

Expression of ttm50^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 results in fragmented spherical mitochondrial morphology as well as shortening of the dendritic arbor in the class IV ddaC neurons in third instar larvae. ttm50^{Scer\UAS.T:Ivir\HA1} expression driven by either Scer\GAL4²²¹ or Scer\GAL4^smid-C161 also leads to moderate dendrite loss.

(Tsuyama et al., 2017)

Expression of ttm50^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C can result in lethality, depending on the P{UAS-ttm50.HA} insertion line used.

Expression of ttm50^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF results in a mild rough eye phenotype.

(Sugiyama et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has visible phenotype, enhanceable by Diap1^unspecified/th[+]

(Sugiyama et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^109(2)80, ttm50^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has visible phenotype, suppressible by Df(3L)H99/+

(Sugiyama et al., 2007)

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has visible phenotype, suppressible by W[+]/hid^unspecified

(Sugiyama et al., 2007)

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has visible phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Sugiyama et al., 2007)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, ttm50^UAS.Tag:HA has abnormal neuroanatomy | pupal stage phenotype, non-suppressible by CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

Scer\GAL4^ppk.PG, ttm50^UAS.Tag:HA has decreased size | pupal stage phenotype, non-suppressible by CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

Suppressor of

Statement

Reference

ttm50^UAS.Tag:HA, Scer\GAL4^ppk.PG is a suppressor of abnormal neuroanatomy | pupal stage phenotype of CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

ttm50^UAS.Tag:HA, Scer\GAL4^ppk.PG is a suppressor of increased size | pupal stage phenotype of CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, ttm50^UAS.Tag:HA has increased occurrence of cell division | larval stage phenotype

(Sugiyama et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has eye phenotype, enhanceable by Diap1^unspecified/th[+]

(Sugiyama et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^109(2)80, ttm50^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, ttm50^UAS.Tag:HA has dendrite | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has eye phenotype, suppressible by Df(3L)H99/+

(Sugiyama et al., 2007)

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has eye phenotype, suppressible by W[+]/hid^unspecified

(Sugiyama et al., 2007)

Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Sugiyama et al., 2007)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, ttm50^UAS.Tag:HA has larval multidendritic class IV neuron | pupal stage phenotype, non-suppressible by CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

Scer\GAL4^ppk.PG, ttm50^UAS.Tag:HA has dendrite | pupal stage phenotype, non-suppressible by CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

Suppressor of

Statement

Reference

ttm50^UAS.Tag:HA, Scer\GAL4^ppk.PG is a suppressor of larval multidendritic class IV neuron | pupal stage phenotype of CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

ttm50^UAS.Tag:HA, Scer\GAL4^ppk.PG is a suppressor of dendrite | pupal stage phenotype of CalpA^JF03391, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF, ttm50^UAS.Tag:HA has ommatidium | increased number phenotype

(Sugiyama et al., 2007)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, ttm50^UAS.Tag:HA has imaginal disc phenotype

(Sugiyama et al., 2007)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, ttm50^UAS.Tag:HA has wing disc | larval stage phenotype

(Sugiyama et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of the dendritic arbor in class IV ddaC neurons observed in third instar larvae expressing ttm50^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 can be rescued by co-expression of PPP1R15^Scer\UAS.cMa.

(Tsuyama et al., 2017)

The rough eye phenotype caused by expression of ttm50^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is suppressed by Df(3L)H99/+ or W^unspecified/+ and enhanced by th^unspecified/+.

(Sugiyama et al., 2007)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa almost completely suppresses the rough eye phenotype caused by expression of ttm50^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF, and in addition, the total number of ommatidia is increased in these flies.

Co-expression of BacA\p35^Scer\UAS.cHa and ttm50^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E results in an increase in nuclear density and an increase in cell proliferation (as assayed by BrdU incorporation) in the imaginal disc.

(Sugiyama et al., 2007)

Complementation and Rescue Data

Rescues

ttm50^UAS.Tag:HA/Scer\GAL4^unspecified rescues ttm50^IE1

(Sugiyama et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ttm50^{Scer\UAS.T:Ivir\HA1}

ttm50^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (3)

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