Open Close
General Information
Symbol
Scer\GAL4c305a
Species
S. cerevisiae
Name
FlyBase ID
FBal0215258
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
c305a-GAL4, c305a, c305-Gal4, GAL4-c305a, c305aGAL4
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    transposable element insertion site
    (Brown et al., 2019, Hamid et al., 2019, Jia et al., 2019, Marmor-Kollet et al., 2019, Sengupta et al., 2019, Al-Anzi and Zinn, 2018, Louis et al., 2018, Miyashita et al., 2018, Sun et al., 2018, Sun et al., 2018, Valadas et al., 2018, Widmer et al., 2018, Widmer et al., 2018, Aranda et al., 2017, Marchetti and Tavosanis, 2017, Ries et al., 2017, Cervantes-Sandoval et al., 2016, Drago and Davis, 2016, Gai et al., 2016, Kelly et al., 2016, Liu et al., 2016, Scholz-Kornehl and Schwärzel, 2016, Yang et al., 2016, Agrawal and Hasan, 2015, Bai and Sehgal, 2015, Bouzaiane et al., 2015, Chen et al., 2015, Haynes et al., 2015, Kirkhart and Scott, 2015, Krüttner et al., 2015, Lewis et al., 2015, Reynaud et al., 2015, Shankar et al., 2015, Siegenthaler et al., 2015, Andlauer et al., 2014, Boto et al., 2014, Lin et al., 2014, Vogt et al., 2014, Cavaliere et al., 2013, Fitzsimons et al., 2013, Lin et al., 2013, Malik et al., 2013, Melnattur et al., 2013, Pech et al., 2013, Perrat et al., 2013, Riemensperger et al., 2013, Thran et al., 2013, Ueno et al., 2013, Xie et al., 2013, Zhang et al., 2013, Butcher et al., 2012, Chen et al., 2012, Keleman et al., 2012, Kucherenko et al., 2012, Scheunemann et al., 2012, Tonoki and Davis, 2012, Christiansen et al., 2011, Lee et al., 2011, Buchanan and Davis, 2010, Hekmat-Scafe et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Shuai et al., 2010, Tan et al., 2010, Aso et al., 2009, Blum et al., 2009, Leiss et al., 2009, Lin et al., 2009, Mao and Davis, 2009, van Swinderen et al., 2009, Keleman et al., 2007, Krashes et al., 2007, Lin et al., 2007, Armstrong et al., 2006)
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    P{GawB}Ckac305a
    Product class / Tool use(s)
    Expression Data
    Reporter Expression
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    neuron of mushroom body alpha'-lobe

    Comment: reported as 4 days after hatching

    neuron of mushroom body beta'-lobe

    Comment: reported as 4 days after hatching

    immunolocalization
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Information
    Statement
    Reference
    Drives expression in alpha' and beta' lobes of the mushroom body, and in alpha'/beta' mushroom body neurons.
    Scer\GAL4c305a labels a large cluster of excitatory local interneurons with cell bodies located lateral to the antennal lobe. Their axons fasciculate and enter the antennal lobe.
    Expression of Scer\GAL4c305a was observed in the alpha' and beta' lobes of the mushroom body at 4 days after larval hatching (late wandering third instar), but not earlier at 2 days after hatching (early third instar).
    Scer\GAL4c305a labels the mushroom body alpha'/beta' neurons. Co-expression with a postsynaptic marker labels Kenyon cell terminals in the mushroom body alpha' and beta' lobes. No presynaptic terminals in the calyx are observed.
    This line labels larval-born alpha' and beta' Kenyon cells in the third instar as well as elements in the thoracic and abdominal ganglia, mostly sensory terminals from the periphery.
    Expression is observed in about half of the the alpha', and beta' neurons of the adult mushroom body, in ellipsoid body neurons and in the antennal lobe.
    Expression of Scer\GAL4c305a in the adult antennal nerve is in putative mechanosensory neurons, and within the ring neurons of the ellipsoid body.
     
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4c305a cells in adults significantly reduces startle-induced climbing behavior, compared to controls. Thermogenetic activation (using TrpA1UAS.(B).cKa at 32[o]C) of Scer\GAL4c305a cells in adults significantly reduces startle-induced climbing behavior, compared to controls.
    Inhibition (using shi1.UAS at 31[o]C) of Scer\GAL4c305a cells in adults during consolidation only, but not testing only, significantly reduces 3-hour aversive olfactory anaesthesia-resistant memory, compared to controls.
    Activation of Scer\GAL4c305a-expressing mushroom body α/β neurons results in a strong decrease in nighttime sleep. This nighttime sleep loss remains stable over 48 hours of activation and is accompanied by increasing daytime sleep loss.
    Inhibition of neural activity in Scer\GAL4c305a-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.
    Inhibition (using shi1.UAS at 33[o]C) of Scer\GAL4c305a cells in adults significantly reduces aversive 1-min olfactory, but not visual, memory, compared to controls.
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT suppressed by
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Symbol Synonym
    Scer\GAL4c305a
    Name Synonyms
    Secondary FlyBase IDs
      References (76)