FlyBase Allele Report: Scer\GAL4[c305a]

General Information

Symbol

Scer\GAL4^c305a

Species

S. cerevisiae

Name

FlyBase ID

FBal0215258

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}Cka^c305a

Carried in Construct

Also Known As

c305a-GAL4, c305a, c305-Gal4, c305aGAL4, GAL4-c305a, C305

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}Cka^c305a

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,031,086..8,031,086 [-]

(Chu et al., 2024, Delescluse et al., 2024, Draper et al., 2024, Lancaster et al., 2024, Chen et al., 2023, Modi et al., 2023, Noyes and Davis, 2023, Schwartz et al., 2023, Shen et al., 2023, Bertin et al., 2022, Foka et al., 2022, Grover et al., 2022, Manoim et al., 2022, Sabandal et al., 2022, Hamid et al., 2021, Hatch et al., 2021, Inami et al., 2021, Kim et al., 2021, Sabandal et al., 2021, Wu et al., 2021, Yang et al., 2021, Yatsenko et al., 2021, Sabandal et al., 2020, Silva et al., 2020, Wu et al., 2020, Bielopolski et al., 2019, Brown et al., 2019, Deng et al., 2019, Hamid et al., 2019, Higham et al., 2019, Jia et al., 2019, Marmor-Kollet et al., 2019, Papanikolopoulou et al., 2019, Roussou et al., 2019, Sengupta et al., 2019, Al-Anzi and Zinn, 2018, Louis et al., 2018, Miyashita et al., 2018, Sun et al., 2018, Sun et al., 2018, Valadas et al., 2018, Widmer et al., 2018, Widmer et al., 2018, Aranda et al., 2017, Marchetti and Tavosanis, 2017, Ries et al., 2017, Saras et al., 2017, Cervantes-Sandoval et al., 2016, Drago and Davis, 2016, Gai et al., 2016, Jiang et al., 2016, Kelly et al., 2016, Liu et al., 2016, Scholz-Kornehl and Schwärzel, 2016, Tong et al., 2016, Yang et al., 2016, Agrawal and Hasan, 2015, Bai and Sehgal, 2015, Bouzaiane et al., 2015, Chen et al., 2015, Haynes et al., 2015, Kirkhart and Scott, 2015, Krüttner et al., 2015, Lewis et al., 2015, Reynaud et al., 2015, Shankar et al., 2015, Siegenthaler et al., 2015, Andlauer et al., 2014, Boto et al., 2014, Dunne et al., 2014, Lin et al., 2014, Vogt et al., 2014, Cavaliere et al., 2013, Fitzsimons et al., 2013, Lin et al., 2013, Malik et al., 2013, Melnattur et al., 2013, Pech et al., 2013, Perrat et al., 2013, Riemensperger et al., 2013, Thran et al., 2013, Ueno et al., 2013, Xie et al., 2013, Zhang et al., 2013, Butcher et al., 2012, Chen et al., 2012, Keleman et al., 2012, Kucherenko et al., 2012, Scheunemann et al., 2012, Tonoki and Davis, 2012, Christiansen et al., 2011, Lee et al., 2011, Buchanan and Davis, 2010, Hekmat-Scafe et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Shuai et al., 2010, Tan et al., 2010, Aso et al., 2009, Blum et al., 2009, Leiss et al., 2009, Lin et al., 2009, Mao and Davis, 2009, van Swinderen et al., 2009, Keleman et al., 2007, Krashes et al., 2007, Lin et al., 2007, Armstrong et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

core layer of the larval mushroom body

(Pauls et al., 2010)

wandering third instar larval stage

neuron of adult mushroom body alpha'-lobe

Comment: reported as 4 days after hatching

(Lin et al., 2013)

neuron of adult mushroom body beta'-lobe

Comment: reported as 4 days after hatching

(Lin et al., 2013)

adult stage

adult antennal lobe

(Aso et al., 2009, Krashes et al., 2007)

ellipsoid body | restricted

(Krashes et al., 2007)

adult antennal nerve | restricted

(Krashes et al., 2007)

pedunculus of adult mushroom body | distal | restricted

(Mao and Davis, 2009)

alpha'/beta' Kenyon cell

(Christiansen et al., 2011, Buchanan and Davis, 2010)

neuron | subset of adult brain

(Chen et al., 2012)

adult mushroom body

Comment: medium expression

(Chen et al., 2012)

glial cell of adult brain

Comment: faint expression

(Chen et al., 2012)

ellipsoid body

Comment: medium expression

(Chen et al., 2012, Aso et al., 2009)

eye

Comment: faint expression

(Chen et al., 2012)

adult mushroom body beta'-lobe

(Riemensperger et al., 2013, Pauls et al., 2010, Tan et al., 2010, Mao and Davis, 2009, Krashes et al., 2007)

adult mushroom body alpha'-lobe

(Riemensperger et al., 2013, Pauls et al., 2010, Tan et al., 2010, Krashes et al., 2007)

mushroom body alpha' middle layer

(Aso et al., 2009)

mushroom body beta' middle layer

(Aso et al., 2009)

mushroom body alpha' anterior-posterior layer | intense

(Aso et al., 2009)

mushroom body beta' anterior-posterior layer | intense

(Aso et al., 2009)

medulla | faint

(Aso et al., 2009)

adult protocerebrum | restricted

(Aso et al., 2009)

inferior neuropils

(Aso et al., 2009)

adult antennal nerve | intense

(Aso et al., 2009)

adult tritocerebrum

(Aso et al., 2009)

adult gnathal ganglion

(Aso et al., 2009)

adult pars intercerebralis | faint

(Aso et al., 2009)

adult stage | male

adult mushroom body alpha'-lobe

(Blum et al., 2009)

adult mushroom body beta'-lobe

(Blum et al., 2009)

ellipsoid body

(Blum et al., 2009)

antennal lobe

(Blum et al., 2009)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult antennal lobe local neuron

(Melnattur et al., 2013)

Additional Information

Statement

Reference

Drives expression in alpha' and beta' lobes of the mushroom body, and in alpha'/beta' mushroom body neurons.

(FlyBase Curators, 2017)

ScerGAL4^c305a labels a large cluster of excitatory local interneurons with cell bodies located lateral to the antennal lobe. Their axons fasciculate and enter the antennal lobe.

(Melnattur et al., 2013)

Expression of ScerGAL4^c305a was observed in the alpha' and beta' lobes of the mushroom body at 4 days after larval hatching (late wandering third instar), but not earlier at 2 days after hatching (early third instar).

(Lin et al., 2013)

ScerGAL4^c305a labels the mushroom body alpha'/beta' neurons. Co-expression with a postsynaptic marker labels Kenyon cell terminals in the mushroom body alpha' and beta' lobes. No presynaptic terminals in the calyx are observed.

(Christiansen et al., 2011)

This line labels larval-born alpha' and beta' Kenyon cells in the third instar as well as elements in the thoracic and abdominal ganglia, mostly sensory terminals from the periphery.

(Pauls et al., 2010)

Expression is observed in about half of the the alpha', and beta' neurons of the adult mushroom body, in ellipsoid body neurons and in the antennal lobe.

(Blum et al., 2009)

Expression of ScerGAL4^c305a in the adult antennal nerve is in putative mechanosensory neurons, and within the ring neurons of the ellipsoid body.

(Krashes et al., 2007)

Marker for

adult mushroom body alpha'-lobe

(Krashes et al., 2007)

adult mushroom body beta'-lobe

(Krashes et al., 2007)

alpha'/beta' Kenyon cell

(Christiansen et al., 2011)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | third instar larval stage, with ChT^JF03028

(Hamid et al., 2021)

abnormal chemosensory behavior | third instar larval stage, with ChT^KK109385

(Hamid et al., 2021)

abnormal chemosensory behavior | third instar larval stage, with shi^ts.UAS.cUa

(Hamid et al., 2021)

abnormal learning, with shi^1.UAS

(Pauls et al., 2010)

abnormal learning | third instar larval stage, with ChT^JF03028

(Hamid et al., 2021)

abnormal learning | third instar larval stage, with ChT^KK109385

(Hamid et al., 2021)

abnormal learning | third instar larval stage, with shi^ts.UAS.cUa

(Hamid et al., 2021)

abnormal locomotor behavior | adult stage | heat sensitive, with shi^1.UAS

(Sun et al., 2018, Riemensperger et al., 2013)

abnormal locomotor behavior | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Sun et al., 2018, Riemensperger et al., 2013)

abnormal memory, with CaMKII^RNAi.UAS

(Malik et al., 2013)

abnormal memory, with CaMKII^T287A.UAS

(Malik et al., 2013)

abnormal memory, with CaMKII^T287D.UAS

(Malik et al., 2013)

abnormal memory, with CaMKII^{T306A.T307A.UAS}

(Malik et al., 2013)

abnormal memory, with CaMKII^UAS.cKa

(Malik et al., 2013)

abnormal memory, with CASK^KK109311

(Malik et al., 2013)

abnormal memory, with CASK^UAS.cBa

(Malik et al., 2013)

abnormal memory | adult stage, with KCNQ^JF02562

(Cavaliere et al., 2013)

abnormal memory | adult stage, with shi^1.UAS

(Krashes et al., 2007)

abnormal memory | adult stage, with SLC22A^KK112539

(Gai et al., 2016)

abnormal memory | adult stage, with tau^RNAi.UAS

(Papanikolopoulou et al., 2019)

abnormal memory | adult stage | :heat sensitive | conditional, with shi^1.UAS

(Vogt et al., 2014)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Yang et al., 2016)

abnormal neuroanatomy, with Fas2^UAS.cLa

(Kucherenko et al., 2012)

abnormal neuroanatomy | P-stage, with mamo^RNAi.DG.UAS

(Chu et al., 2024)

abnormal neuroanatomy | P-stage, with Sema1a^HMS01307

(Chu et al., 2024)

abnormal neurophysiology | heat sensitive, with shi^1.UAS

(van Swinderen et al., 2009)

abnormal pain response | adult stage, with tau^RNAi.UAS

(Papanikolopoulou et al., 2019)

abnormal sleep, with GABA-B-R3^GD578

(Haynes et al., 2015)

abnormal sleep, with nkt^GD8239

(Sengupta et al., 2019)

abnormal sleep, with Rdl^{RNAi.8-10.UAS}

(Haynes et al., 2015)

abnormal visual behavior | heat sensitive, with shi^1.UAS

(van Swinderen et al., 2009)

decreased fecundity, with Nf1^P1, Nf1^UAS.cBa

(Buchanan and Davis, 2010)

lethal, with trio^{GEF1.UAS.Tag:MYC}

(Lancaster et al., 2024)

Phenotype Manifest In

adult Kenyon cell | P-stage, with mamo^RNAi.DG.UAS

(Chu et al., 2024)

adult Kenyon cell | P-stage, with Sema1a^HMS01307

(Chu et al., 2024)

axon | P-stage, with mamo^RNAi.DG.UAS

(Chu et al., 2024)

axon | P-stage, with Sema1a^HMS01307

(Chu et al., 2024)

lobe system of adult mushroom body, with Fas2^UAS.cLa

(Kucherenko et al., 2012)

Detailed Description

Statement

Reference

The inhibition of α'/β' class Kenyon cells by the expression of shi^ts.UAS.cUa under the control of Scer\GAL4^c305a at the restrictive temperature of 29[o]C leads to third instar foraging larvae showing increased naive chemotactic response, as well as decreased olfactory habituation, to ethyl acetate.

(Hamid et al., 2021)

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^c305a cells in adults significantly reduces startle-induced climbing behavior, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^c305a cells in adults significantly reduces startle-induced climbing behavior, compared to controls.

(Sun et al., 2018)

Inhibition (using shi^1.UAS at 31[o]C) of Scer\GAL4^c305a cells in adults during consolidation only, but not testing only, significantly reduces 3-hour aversive olfactory anaesthesia-resistant memory, compared to controls.

(Yang et al., 2016)

Activation of Scer\GAL4^c305a-expressing mushroom body α/β neurons results in a strong decrease in nighttime sleep. This nighttime sleep loss remains stable over 48 hours of activation and is accompanied by increasing daytime sleep loss.

(Haynes et al., 2015)

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^c305a cells in adults does not affect 5 min (single-trial conditioned) or 30 min (multiple-trial conditioned) aversive taste memory, compared to controls.

(Kirkhart and Scott, 2015)

Inhibition of neural activity in Scer\GAL4^c305a-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^c305a cells in adults significantly reduces aversive 1-min olfactory, but not visual, memory, compared to controls.

(Vogt et al., 2014)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^c305a cells in adults during testing does not affect 3 h aversive or appetitive memory, compared to controls.

(Xie et al., 2013)

Inhibition (using shi^1.UAS at 31[o]C) of Scer\GAL4^c305a cells in adults does not affect short-term aversive olfactory or visual unimodal memory, compared to controls; inhibition does not affect short-term aversive memory for tested olfactory cues that are associated with conditioned visual cues, compared to controls.

(Zhang et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^c305a, mamo^RNAi.DG.UAS has abnormal neuroanatomy | P-stage phenotype, suppressible | partially by Scer\GAL4^c305a/Sema1a^UAS.cSa

(Chu et al., 2024)

NOT suppressed by