Allele Dmel\ab^{Scer\UAS.L.cSa}

General Information
Symbol	Dmel\ab^{Scer\UAS.L.cSa}	Species	D. melanogaster
Name		FlyBase ID	FBal0215327
Feature type	allele	Associated gene	Dmel\ab

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Sugimura et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequnces drive expression of the 904 amino acid isoform of ab. The ab sequence includes 164bp of 5' UTR and 213bp of 3'UTR in addition to the entire coding sequence. (Sugimura et al., 2004)
Carried in construct	P{UAS-ab.L} (Sugimura et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^109(2)80, Scer\GAL4^IG1-2 (Sugimura et al., 2004)
Phenotype Manifest In
	dorsal multidendritic neuron ddaA, with Scer\GAL4^109(2)80, Scer\GAL4^IG1-2 (Sugimura et al., 2004) dorsal multidendritic neuron ddaB, with Scer\GAL4^109(2)80, Scer\GAL4^IG1-2 (Sugimura et al., 2004) dorsal multidendritic neuron ddaC, with Scer\GAL4^109(2)80, Scer\GAL4^IG1-2 (Sugimura et al., 2004)
Detailed Description
	Statement Reference Expression of ab^{Scer\UAS.L.cSa} in all da neurons, driven by Scer\GAL4^109(2)80 and Scer\GAL4^IG1-2 together, causes an alteration of overall patterns of dendritic arbors in larvae. These larvae show large dendrite-free zones around segmental boundaries and dorsal midlines, which lie midway between ipsilateral and contralateral dorsal clusters, while control larvae show dendritic trees over all of the body wall. The total number and the stereotypic arrangement of da neurons is not changed in the ab^{Scer\UAS.L.cSa}-expressing larvae, suggesting that class II-IV dendritic trees are reduced in size. This downsizing effect occurs with complete penetrance. Single-cell analysis on ddaC neurons that express ab^{Scer\UAS.L.cSa} under the control of Scer\GAL4^109(2)80 and Scer\GAL4^IG1-2 show a reduced dendritic field and exhibit a visibly less complex morphology compared to wild type. The branch terminals of the mutant ddaC do not reach far enough to meet those of adjacent cells and higer-order fine branches are not elaborated. Time-lapse analysis shows that ab^{Scer\UAS.L.cSa}-expressing ddaC neurons almost halt branching and stabilize their overall dendritic shape by midlarval stages. Expression of ab^{Scer\UAS.L.cSa} under the control of Scer\GAL4^109(2)80 and Scer\GAL4^IG1-2 results in a morphological change in ddaA neurons as they do not show the characteristic short, straight protrusions that wild-type ddaA neurons exhibit. The ab^{Scer\UAS.L.cSa}-expressing ddA neurons also show a lower terminal number than controls and a significant decrease in the field size. The size of ddaB dendritic fields is also reduced in this genotype but the number of ddaB branch terminals is not altered. The dendritic field size and number of branch terminals is not affected in either ddaD or ddaE neurons. (Sugimura et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^IG1-1/ab^{Scer\UAS.L.cSa} rescues ab^clu-1 (Sugimura et al., 2004) Scer\GAL4^IG1-1/ab^{Scer\UAS.L.cSa} rescues ab^k02807/ab^clu-1 (Sugimura et al., 2004)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ab^{Scer\UAS.L.cSa}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Sugimura et al., 2004, Neuron 43(6): 809--822 Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt. [FBrf0180576]

Allele Dmel\abScer\UAS.L.cSa

Allele Dmel\ab^{Scer\UAS.L.cSa}