Expression of Rab1S25N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4Ilp2.PW results in a dramatic (>40%) reduction in adult fly weight. Pupal size is also dramatically reduced and flies eclose, on average, ~2 days later than control flies. Hemolymph levels of trehalose and glucose are elevated compared to controls. Fewer Insulin Producing Cells (IPCs) are seen in the larvae and IPC neuronal morphology is dramatically disrupted. Dendritic arborisations are missing while the axonal bundles are mostly intact. Limiting Rab1S25N.Scer\UAS.T:Avic\GFP-YFP expression to late larval development onwards using Scer\GAL80ts.αTub84B avoids the IPC developmental defects.
The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab1S25N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ are not significantly different from those seen in control hemocytes.