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General Information
Symbol
Dmel\Rab1S25N.UAS.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215381
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Rab1DN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: S25N.

UASt regulatory sequences drive expression of a predicted dominant negative form of Rab1 that is tagged at the N-terminal end with YFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When Rab1S25N.Scer\UAS.T:Avic\GFP-YFP is expressed using Scer\GAL4ppk.PG, transgenic animals show defective nociception response.

Expression of Rab1S25N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4Ilp2.PW results in a dramatic (>40%) reduction in adult fly weight. Pupal size is also dramatically reduced and flies eclose, on average, ~2 days later than control flies. Hemolymph levels of trehalose and glucose are elevated compared to controls. Fewer Insulin Producing Cells (IPCs) are seen in the larvae and IPC neuronal morphology is dramatically disrupted. Dendritic arborisations are missing while the axonal bundles are mostly intact. Limiting Rab1S25N.Scer\UAS.T:Avic\GFP-YFP expression to late larval development onwards using Scer\GAL80ts.αTub84B avoids the IPC developmental defects.

The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab1S25N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ are not significantly different from those seen in control hemocytes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Rab1S25N.Scer\UAS.T:Avic\GFP-YFP
Rab1S25N.UAS.YFP
Name Synonyms
Secondary FlyBase IDs
    References (13)