Allele Dmel\Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}

General Information
Symbol	Dmel\Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0215394
Feature type	allele	Associated gene	Dmel\Rab5

Allele class
Mutagen	in vitro construct - amino acid replacement, in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Zhang et al., 2007, Zhang et al., 2007) in vitro construct - coding region fusion (Zhang et al., 2007) in vitro construct - regulatory fusion (Zhang et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q88L. (Zhang et al., 2007) Scer\UAS regulatory and P\T promoter sequences drive expression of a predicted constitutively active form of Rab5 which is tagged at the N-terminal end with T:Avic\GFP^YFP. (Zhang et al., 2007)
Carried in construct	P{UASp-YFP.Rab5.Q88L} (Warner and Longmore, 2009, Woolworth et al., 2009, Zhang et al., 2007, Zhang et al., 2007, Gailite et al., 2012, Hsouna et al., 2010, Yuan et al., 2010, Uytterhoeven et al., 2011, Gault et al., 2012)
Tagged with	T:Avic\GFP^YFP (Zhang et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective, with Scer\GAL4^n-syb.PS (Uytterhoeven et al., 2011)
Phenotype Manifest In
	early endosome, with Scer\GAL4^CU1 (Gailite et al., 2012) embryonic/larval neuromuscular junction, with Scer\GAL4^n-syb.PS (Uytterhoeven et al., 2011) follicle cell, with Scer\GAL4^55B (Woolworth et al., 2009) muscle tendon junction, with Scer\GAL4^Mef2.PR (Yuan et al., 2010) wing hair \| supernumerary, with Scer\GAL4^en-e16E (Gault et al., 2012)
Detailed Description
	Statement Reference Expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] using Scer\GAL4[CU1] induces formation of enlarged early endosomes. (Gailite et al., 2012) Expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] via Scer\GAL4[en-e16E] results only rarely in multiple wing hairs. (Gault et al., 2012) Excitatory junctional current amplitude and quantal content at the neuromuscular junction are increased compared to controls in third instar larvae expressing Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] under the control of Scer\GAL4[n-syb.PS]. (Uytterhoeven et al., 2011) Embryos expressing Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] under the control of Scer\GAL4[Mef2.PR] show muscle detachment defects. These defects are due to separation between the integrins at the end of the muscles and the extracellular matrix. (Yuan et al., 2010) Clones expressing Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] (under the control of Scer\GAL4[Act5C.PI]) do not show differences in the adherens junctions. (Warner and Longmore, 2009) Epithelial defects are seen in the egg chambers of females expressing Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] under the control of Scer\GAL4[55B]; 14% show cell swelling. (Woolworth et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Statement Reference Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-enhanceable by Hrs^{2xFYVE.Scer\UAS.T:Avic\GFP}, Scer\GAL4^CU1 (Gailite et al., 2012) Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-enhanceable by rush^{R176G.Scer\UAS.T:Avic\GFP}, Scer\GAL4^CU1 (Gailite et al., 2012)
NOT suppressed by
Statement Reference Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-suppressible by Hrs^{2xFYVE.Scer\UAS.T:Avic\GFP}, Scer\GAL4^CU1 (Gailite et al., 2012) Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-suppressible by rush^{R176G.Scer\UAS.T:Avic\GFP}, Scer\GAL4^CU1 (Gailite et al., 2012)
Suppressor of
Statement Reference Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^55B/Scer\GAL4^55B is a suppressor \| partially of follicle cell phenotype of Scer\GAL4^55B/Scer\GAL4^55B, awd^{dsRNA.Scer\UAS} (Woolworth et al., 2009) Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^btl.PS is a suppressor \| partially of embryonic/larval dorsal trunk phenotype of Scer\GAL4^btl.PS, Vhl^{dsRNA.Scer\UAS} (Hsouna et al., 2010)
NOT Suppressor of
Statement Reference Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} is a non-suppressor of adherens junction \| somatic clone phenotype of Rho1^72F (Warner and Longmore, 2009) Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} is a non-suppressor of adherens junction \| somatic clone phenotype of Rho1^72O (Warner and Longmore, 2009)
Other
Statement Reference Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^CU1, rush^{K48E.Scer\UAS.T:Avic\GFP} has endosome phenotype (Gailite et al., 2012) Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^CU1, rush^{Scer\UAS.T:Avic\GFP} has endosome phenotype (Gailite et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of rush[Scer\UAS.T:Avic\GFP] and Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] using Scer\GAL4[CU1] results in striking changes in endosome morphology: the Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP]-induced large endosomes change their shape and form clusters of smaller interconnected vesicles. Co-expression of rush[K48E.Scer\UAS.T:Avic\GFP] and Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] using Scer\GAL4[CU1] results in endosome clustering. Co-expression of rush[R176G.Scer\UAS.T:Avic\GFP] has no effect on the vesicle shape changes induced by Scer\GAL4[CU1]-mediated expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP]. Co-expression of Hrs[2xFYVE.Scer\UAS.T:Avic\GFP] has no effect on the vesicle shape changes induced by Scer\GAL4[CU1]-mediated expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP]. (Gailite et al., 2012) The penetrance of the tracheal lumen defects (convoluted and dilated dorsal trunks) caused by expression of Vhl[dsRNA.Scer\UAS] under the control of Scer\GAL4[btl.PS] is partially suppressed by co-expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP]. (Hsouna et al., 2010) Expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] (under the control of Scer\GAL4[Act5C.PI]) in Rho1[72O] clones does not worsen the Rho1[72O] adherens junction phenotype between two clonal pigment epithelial cells but does disrupt adherens junctions between a pigment epithelial cell and cone cell, a phenotype that is not found in Rho1[72O] clones. Expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP] (under the control of Scer\GAL4[Act5C.PI]) in Rho1[72F] clones does not worsen the Rho1[72F] adherens junction phenotype between two clonal pigment epithelial cells but does disrupt adherens junctions between a pigment epithelial cell and cone cell, a phenotype taht is not found in Rho1[72F] clones. (Warner and Longmore, 2009) The follicle cell epithelial defects seen in females expressing awd[dsRNA.Scer\UAS] under the control of Scer\GAL4[55B] are rescued by co-expression of Rab5[Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP]. (Woolworth et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	9773 w¹¹¹⁸; P{UASp-YFP.Rab5.Q88L}01a/TM3, Ser¹ 9774 y¹ w^*; P{UASp-YFP.Rab5.Q88L}Reph²⁴
Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2R+ cells. (Zhang et al., 2007)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab5^Q88L (Woolworth et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Gailite et al., 2012, Mol. Biol. Cell 23(3): 433--447 The phosphoinositide-associated protein Rush hour regulates endosomal trafficking in Drosophila. [FBrf0217308] Gault et al., 2012, J. Cell Biol. 196(5): 605--621 Drosophila CK1-γ, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. [FBrf0217630] Chan et al., 2011, Curr. Biol. 21(20): 1704--1715 Systematic discovery of rab GTPases with synaptic functions in Drosophila. [FBrf0216517] Uytterhoeven et al., 2011, Cell 145(1): 117--132 Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins. [FBrf0213384] Hsouna et al., 2010, Mol. Cell. Biol. 30(15): 3779--3794 Drosophila von hippel-lindau tumor suppressor gene function in epithelial tubule morphogenesis. [FBrf0211250] Yuan et al., 2010, J. Cell Sci. 123(6): 939--946 Analysis of integrin turnover in fly myotendinous junctions. [FBrf0213429] Warner and Longmore, 2009, J. Cell Biol. 185(6): 1111--1125 Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. [FBrf0208183] Woolworth et al., 2009, Mol. Cell. Biol. 29(17): 4679--4690 The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis. [FBrf0208712] Zhang et al., 2007, Genetics 176(2): 1307--1322 Thirty-one flavors of Drosophila Rab proteins. [FBrf0201049]
Supplementary material	Zhang et al., 2007, Genetics 176(2): Supplement to Zhang et al., 2007. [FBrf0203184]

Allele Dmel\Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP

Allele Dmel\Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}