FlyBase Allele Report: Dmel\Rab5[Q88L.UASp.YFP]

General Information

Symbol

Dmel\Rab5^{Q88L.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215394

Feature type

allele

Associated gene

Dmel\Rab5

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab5.Q88L}

Also Known As

Rab5^CA, UAS-YFP-Rab5-Q88L, UAS-YFP.Rab5.Q88L, UAS-YFP-Rab5^ca, UAS-Rab5-CA, UAS-YFP-Rab5^Q88L

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab5.Q88L}

Cytology

Description

Amino acid replacement: Q88L.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab5 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab5

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^nSyb.PS

(Uytterhoeven et al., 2011)

increased size | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Lattao et al., 2021)

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr-MD237

(Thomas et al., 2009)

early endosome, with Scer\GAL4^CU1

(Gailite et al., 2012)

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

(Liu et al., 2015)

embryonic/larval neuromuscular junction, with Scer\GAL4^nSyb.PS

(Uytterhoeven et al., 2011)

endosome, with Scer\GAL4^Bx-MS1096

(Yamazaki et al., 2013)

filamentous actin | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Yu et al., 2024)

follicle cell, with Scer\GAL4^55B

(Woolworth et al., 2009)

muscle tendon junction, with Scer\GAL4^Mef2.PR

(Yuan et al., 2010)

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

(Yu et al., 2024)

scutellar bristle, with Scer\GAL4^pnr-MD237

(Thomas et al., 2009)

vesicle, with Scer\GAL4^eg-Mz360

(van den Brink et al., 2013)

vesicle | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

(Liu et al., 2015)

vesicle | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

(Yu et al., 2024)

wing hair | increased number, with Scer\GAL4^en-e16E

(Gault et al., 2012)

yolk granule, with Scer\GAL4^{VP16.mat.αTub67C}

(Yu et al., 2024)

yolk granule | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Lattao et al., 2021)

Detailed Description

Statement

Reference

Embryos from mothers expressing Rab5^{Q88L.UASp.YFP} under the control of Scer\GAL4^{VP16.mat.αTub67C} show larger yolk granules.

(Lattao et al., 2021)

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Appl.G1a does not impair axonal transport in the segmental nerves in third instar larvae.

(Xu et al., 2016)

Expression of Rab5^HMC03420 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in formation of abnormal large vesicular structures in the egg chambers.

(Liu et al., 2015)

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Bx-MS1096 results in enlarged endosomes.

(Yamazaki et al., 2013)

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of either Scer\GAL4^eg-Mz360, Scer\GAL4^eve.RN2 or Scer\GAL4^elav-C155 does not result in any defects in axon outgrowth in the embryo.

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^eg-Mz360 results in an increase in vesicle size in commissural neurons.

(van den Brink et al., 2013)

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} using Scer\GAL4^CU1 induces formation of enlarged early endosomes.

(Gailite et al., 2012)

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} via Scer\GAL4^en-e16E results only rarely in multiple wing hairs.

(Gault et al., 2012)

Excitatory junctional current amplitude and quantal content at the neuromuscular junction are increased compared to controls in third instar larvae expressing Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^n-syb.PS.

(Uytterhoeven et al., 2011)

Embryos expressing Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Mef2.PR show muscle detachment defects. These defects are due to separation between the integrins at the end of the muscles and the extracellular matrix.

(Yuan et al., 2010)

Expression via Scer\GAL4^pnr-MD237 results in a cleft thorax and shortened scutellar macrochaetae.

(Thomas et al., 2009)

Clones expressing Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} (under the control of Scer\GAL4^Act5C.PI) do not show differences in the adherens junctions.

(Warner and Longmore, 2009)

Epithelial defects are seen in the egg chambers of females expressing Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^55B; 14% show cell swelling.

(Woolworth et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-enhancer of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-enhancer of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Suppressor of

Statement

Reference

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^GMR57C10 is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^GH146 is a suppressor | partially of abnormal neuroanatomy | somatic clone phenotype of Strip^dogi

(Sakuma et al., 2014)

NOT Suppressor of

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-suppressor of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Scer\GAL4^C587/Rab5^{Q88L.UASp.YFP} is a non-suppressor of abnormal size | recessive | male | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Appl.G1a is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^GMR57C10 is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Other

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^αTub84B.PL, mtm^null has increased cell number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-enhanceable by rush^{R176G.UAS.GFP}, Scer\GAL4^CU1

(Gailite et al., 2012)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-enhanceable by Hrs^{2xFYVE.UAS.GFP}, Scer\GAL4^CU1

(Gailite et al., 2012)

NOT suppressed by

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-suppressible by rush^{R176G.UAS.GFP}, Scer\GAL4^CU1

(Gailite et al., 2012)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^CU1 has early endosome phenotype, non-suppressible by Hrs^{2xFYVE.UAS.GFP}, Scer\GAL4^CU1

(Gailite et al., 2012)

NOT Enhancer of

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-enhancer of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-enhancer of larval neuroblast | larval stage | increased number phenotype of Vps35^E42

(Li et al., 2018)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-enhancer of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Suppressor of

Statement

Reference

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^GH146 is a suppressor | partially of adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype of Strip^dogi

(Sakuma et al., 2014)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^btl.PS is a suppressor | partially of embryonic/larval tracheal dorsal trunk phenotype of Scer\GAL4^btl.PS, Vhl^RNAi.UAS

(Hsouna et al., 2010)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^55B is a suppressor | partially of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

NOT Suppressor of

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-suppressor of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Scer\GAL4^C587/Rab5^{Q88L.UASp.YFP} is a non-suppressor of gonad | male | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Scer\GAL4^C587/Rab5^{Q88L.UASp.YFP} is a non-suppressor of male germline stem cell | third instar larval stage phenotype of Strip^jam

(La Marca et al., 2019)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a non-suppressor of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^{Q88L.UASp.YFP}/Scer\GAL4^He.PZ is a non-suppressor of hemocyte | increased number | third instar larval stage phenotype of Ocrl^Δ3

(Del Signore et al., 2017)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Appl.G1a is a non-suppressor of larval segmental nerve | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Appl.G1a is a non-suppressor of axon | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Scer\GAL4^Act5C.PI/Rab5^{Q88L.UASp.YFP} is a non-suppressor of adherens junction | somatic clone phenotype of Rho1^72F

(Warner and Longmore, 2009)

Scer\GAL4^Act5C.PI/Rab5^{Q88L.UASp.YFP} is a non-suppressor of adherens junction | somatic clone phenotype of Rho1^72O

(Warner and Longmore, 2009)

Other

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^αTub84B.PL, mtm^null has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Ugt36A1.PK, Spyo\Cas9^UAS.P2, Tbc1d8-9^U6.gRNA.A has nephrocyte phenotype

(Milosavljevic et al., 2022)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^Ugt36A1.PK, Spyo\Cas9^UAS.P2, Tbc1d8-9^U6.gRNA.A has intracellular vesicle phenotype

(Milosavljevic et al., 2022)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^CU1, rush^UAS.GFP has endosome phenotype

(Gailite et al., 2012)

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^CU1, rush^K48E.UAS.GFP has endosome phenotype

(Gailite et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of circulating hemocytes characteristic for Ocrl^Δ3 mutant third instar larvae is not suppressed by Scer\GAL4^He.PZ-driven expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Del Signore et al., 2017)

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 partially rescues the bang sensitivity of para^bss1/+ flies, but does not rescue the bang sensitivity of hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

The axon elongation defect seen in Strip^dogi DL1 olfactory projection neuron single-cell clones is partially suppressed if the cells are also expressing Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} (expression driven by Scer\GAL4^GH146).

(Sakuma et al., 2014)

Co-expression of rush^{Scer\UAS.T:Avic\GFP} and Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} using Scer\GAL4^CU1 results in striking changes in endosome morphology: the Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}-induced large endosomes change their shape and form clusters of smaller interconnected vesicles.

Co-expression of rush^{K48E.Scer\UAS.T:Avic\GFP} and Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} using Scer\GAL4^CU1 results in endosome clustering.

Co-expression of rush^{R176G.Scer\UAS.T:Avic\GFP} has no effect on the vesicle shape changes induced by Scer\GAL4^CU1-mediated expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

Co-expression of Hrs^{2xFYVE.Scer\UAS.T:Avic\GFP} has no effect on the vesicle shape changes induced by Scer\GAL4^CU1-mediated expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Gailite et al., 2012)

The penetrance of the tracheal lumen defects (convoluted and dilated dorsal trunks) caused by expression of Vhl^{dsRNA.Scer\UAS} under the control of Scer\GAL4^btl.PS is partially suppressed by co-expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Hsouna et al., 2010)

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} (under the control of Scer\GAL4^Act5C.PI) in Rho1^72O clones does not worsen the Rho1^72O adherens junction phenotype between two clonal pigment epithelial cells but does disrupt adherens junctions between a pigment epithelial cell and cone cell, a phenotype that is not found in Rho1^72O clones.

Expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} (under the control of Scer\GAL4^Act5C.PI) in Rho1^72F clones does not worsen the Rho1^72F adherens junction phenotype between two clonal pigment epithelial cells but does disrupt adherens junctions between a pigment epithelial cell and cone cell, a phenotype taht is not found in Rho1^72F clones.

(Warner and Longmore, 2009)

The follicle cell epithelial defects seen in females expressing awd^{dsRNA.Scer\UAS} under the control of Scer\GAL4^55B are rescued by co-expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Woolworth et al., 2009)

Xenogenetic Interactions

Statement

Reference

The presence of axonal blockages in the segmental nerves characteristic for third instar larvae expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Appl.G1a is not rescued by co-expression of Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Xu et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9773

w¹¹¹⁸; P{UASp-YFP.Rab5.Q88L}01a/TM3, Ser¹

9774

y¹ w^*; P{UASp-YFP.Rab5.Q88L}Reph²⁴

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2R+ cells.

(Zhang et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Rab5^CA

(Lambert et al., 2022, Kamalesh et al., 2017)

Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab5^{Q88L.UASp.YFP}

Rab5^Q88L

(Woolworth et al., 2009)

Rab5CA

(Sakuma et al., 2014)

Rab5Q88L

(Sakuma et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (44)

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