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General Information
Symbol
Dmel\Rab7T22N.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215399
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
rab7DN, UASp-YFP.Rab7.T22N, Rab7 DN, Rab7-DN, UAS-rab7DN, UAS-YFP.Rab7.T22N
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: T22N.

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab7 that is tagged at the N-terminal end with YFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4He.PZ-driven expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP does not induce increase in the number of circulating hemocytes in third instar larvae relative to controls.

Animals expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4grh.D4 are viable, no obvious defects in larval dorsal trunk elongation and airway morphology are observed and the larvae are of comparable size to age-matched controls.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4btl.PU in embryos does not cause significant defects in dorsal branch fusion.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in formation of abnormal large vesicular structures in the egg chambers.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP in secondary cells under the control of Scer\GAL4dsx.KI reduces exosome secretion into the accessory gland lumen compared to controls.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4CY2 does not change the average lysosome size as compared to wild-type.

Adult hemocytes expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ show slower maturation of phagosomes containing pHrodo-conjugated S. aureus compared to wild-type controls.

Adults expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ show significantly decreased survival compared to controls after injection with S. aureus.

Embryos derived from females expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 show a significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type.

The fraction of oocytes showing endocytic activity is reduced compared to wild type in females expressing Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4eg-Mz360 in commissural axons in the embryo does not disrupt their outgrowth across the midline.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of either Scer\GAL4eve.RN2 or Scer\GAL4elav-C155 does not result in any defects in axon outgrowth in the embryo.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4slbo.2.6 impairs border cell migration in the developing egg chamber. No border cell cluster dissociation is seen.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4CY2 results in a 46% decrease in lysosome clustering in the follicle cells compared to controls.

Expression via Scer\GAL4pnr-MD237 results in flies with a wild type notum.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The increased number of circulating hemocytes along with the increase in F-actin and the expansion of the LysoTracker-positive compartments in the mutant cells characteristic for OcrlΔ3 mutant third instar larvae is partially suppressed by Scer\GAL4He.PZ-driven expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP, but the adult lethality of OcrlΔ3 animals is not rescued.

The lysosome expansion phenotype observed in third instar larvae expressing FIG4dsRNA.miRNA.Scer\UAS under the control of Scer\GAL4Mef2.PU is suppressed by co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP.

Expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4CY2 does not change the average lysosome size in Lrrk mutants.

The reduction in the fraction of oocytes showing endocytic activity caused by expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is suppressed by co-expression of EndoBScer\UAS.P\T.T:Zzzz\FLAG.

The increased clustering of lysosomes which is seen in the follicle cells of females expressing LrrkG1914S.Scer\UAS under the control of Scer\GAL4CY2 is significantly suppressed by co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP.

Co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP weakly suppresses the uninflated wing phenotype of Scer\GAL4Ccap.PP, OsbpScer\UAS.cMa flies.

Xenogenetic Interactions
Statement
Reference

The formation of giant autophagic bodies in the retina of adult flies expressing Hsap\MAPTGMR.Ex.PJ is partially suppressed by co-expression of Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP
Rab7T22N.UASp.YFP
Name Synonyms
Secondary FlyBase IDs
    References (26)