FlyBase Allele Report: Dmel\Rab7[Q67L.UASp.YFP]

General Information

Symbol

Dmel\Rab7^{Q67L.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215400

Feature type

allele

Associated gene

Dmel\Rab7

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab7.Q67L}

Also Known As

rab7^CA, Rab7 CA, UAS-YFP-Rab7-Q67L, UAS-rab7^CA, UAS-Rab7^Q67L

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab7.Q67L}

Cytology

Description

Amino acid replacement: Q67L.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab7 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab7

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased rate of adult locomotory behavior | progressive, with Scer\GAL4^Ddc.PL

(Szinyákovics et al., 2023)

short lived, with Scer\GAL4^Ddc.PL

(Szinyákovics et al., 2023)

Phenotype Manifest In

autophagosome | adult stage, with Scer\GAL4^Ddc.PL

(Szinyákovics et al., 2023)

embryonic/larval hemocyte | increased number | third instar larval stage

(Del Signore et al., 2017)

lysosome, with Scer\GAL4^CY2

(Dodson et al., 2012)

vesicle, with Scer\GAL4^eg-Mz360

(van den Brink et al., 2013)

Detailed Description

Statement

Reference

Scer\GAL4^He.PZ-driven expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} induces moderate increase in the number of circulating hemocytes in third instar larvae relative to controls.

(Del Signore et al., 2017)

The expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^ap-md544 has no obvious effect on the adult wing morphology, as compared to control.

(O'Farrell et al., 2017)

Expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 does not change the average lysosome size as compared to wild-type.

(Dodson et al., 2014)

Expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^eg-Mz360 does not affect extension of the commissural axons in the embryo and the central nervous system forms normally.

Expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^eg-Mz360 results in an increase in vesicle size in commissural neurons.

(van den Brink et al., 2013)

Expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 results in a 35% increase in lysosome clustering in the follicle cells compared to controls. These clusters are primarily perinuclear.

(Dodson et al., 2012)

Expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^en-e16E has no effect on wing hairs.

(Gault et al., 2012)

Expression via Scer\GAL4^pnr-MD237 results in flies with a wild type notum.

(Thomas et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ap-md544 is an enhancer of visible phenotype of Pi3K59F^KD.UAS, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

NOT Enhancer of

Statement

Reference

Rab7^{Q67L.UASp.YFP}/Scer\GAL4^αTub84B.PL is a non-enhancer of increased cell number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-enhancer of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

NOT Suppressor of

Statement

Reference

Rab7^{Q67L.UASp.YFP}/Scer\GAL4^αTub84B.PL is a non-suppressor of increased cell number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-suppressor of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab7^{Q67L.UASp.YFP}/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of increased size | maternal effect | embryonic stage phenotype of Df(3L)R-G7/mv^ros

(Lattao et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^CY2 has lysosome phenotype, enhanceable by Lrrk^e03680

(Dodson et al., 2012)

Suppressed by

Statement

Reference

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^CY2 has lysosome phenotype, suppressible by Lrrk^UAS.cDa, Scer\GAL4^CY2

(Dodson et al., 2012)

Enhancer of

Statement

Reference

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ap-md544 is an enhancer of wing phenotype of Pi3K59F^KD.UAS, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^Mef2.PU is an enhancer of larval somatic muscle cell | third instar larval stage phenotype of FIG4^{RNAi.RNAi.cBa}, Scer\GAL4^Mef2.PU

(Bharadwaj et al., 2016)

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^Mef2.PU is an enhancer of lysosome | third instar larval stage phenotype of FIG4^{RNAi.RNAi.cBa}, Scer\GAL4^Mef2.PU

(Bharadwaj et al., 2016)

NOT Enhancer of

Statement

Reference

Rab7^{Q67L.UASp.YFP}/Scer\GAL4^αTub84B.PL is a non-enhancer of interommatidial cell | P-stage | increased number | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-enhancer of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Rab7^{Q67L.UASp.YFP} is a suppressor of NMJ bouton | increased number | larval stage phenotype of Mon1^Δ181

(Basargekar et al., 2020)

Rab7^{Q67L.UASp.YFP}/Scer\GAL4^RapGAP1-OK6 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Mon1^Δ181

(Basargekar et al., 2020)

NOT Suppressor of

Statement

Reference

Rab7^{Q67L.UASp.YFP}/Scer\GAL4^αTub84B.PL is a non-suppressor of interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ninaE.PT is a non-suppressor of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab7^{Q67L.UASp.YFP}/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of yolk granule | maternal effect | embryonic stage phenotype of Df(3L)R-G7/mv^ros

(Lattao et al., 2021)

Scer\GAL4^CY2/Rab7^{Q67L.UASp.YFP} is a non-suppressor of lysosome phenotype of Lrrk^unspecified

(Dodson et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} enhances the wing defects resulting from the expression of Pi3K59F^KD.Scer\UAS under the control of Scer\GAL4^ap-md544.

(O'Farrell et al., 2017)

The lysosome expansion phenotype observed in third instar larvae expressing FIG4^{dsRNA.miRNA.Scer\UAS} under the control of Scer\GAL4^Mef2.PU is further enhanced by co-expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Bharadwaj et al., 2016)

Expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 does not change the average lysosome size in Lrrk mutants.

(Dodson et al., 2014)

The increased clustering of lysosomes which is seen in the follicle cells of females expressing Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 is enhanced in a Lrrk^e03680 background.

Co-expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} and Lrrk^{G1914S.Scer\UAS} under the control of Scer\GAL4^CY2 results in a degree of lysosome clustering in the follicle cells that is identical to that caused by expression of Lrrk^{G1914S.Scer\UAS} alone under the control of Scer\GAL4^CY2.

(Dodson et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

50785

P{UASp-YFP.Rab7.Q67L}7, y¹ w^*

24103

y¹ w^*; P{UASp-YFP.Rab7.Q67L}19

9779

y¹ w^*; P{UASp-YFP.Rab7.Q67L}awd¹⁴/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Rab7^CA

(Del Signore et al., 2017)

Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab7^{Q67L.UASp.YFP}

Rab7^QL

(Del Signore et al., 2017)

rab7^CA

(Dodson et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (26)

Report Sections

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