FlyBase Allele Report: Dmel\Rab9[S26N.UASp.YFP]

General Information

Symbol

Dmel\Rab9^{S26N.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215405

Feature type

allele

Associated gene

Dmel\Rab9

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab9.S26N}

Also Known As

UAS-Rab9 DN, DN-Rab9

Key Links

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab9.S26N}

Cytology

Description

Amino acid replacement: S26N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab9 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab9

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-enhancer of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-enhancer of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^GMR57C10/Rab9^{S26N.UASp.YFP} is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^nSyb.PS/Rab9^{S26N.UASp.YFP} is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^C23/Rab9^{S26N.UASp.YFP} is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Act5C.PU/Rab9^{S26N.UASp.YFP} is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^GMR57C10/Rab9^{S26N.UASp.YFP} is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-enhancer of larval neuroblast | larval stage | increased number phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-enhancer of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-enhancer of larval neuroblast | larval stage | increased number phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-enhancer of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^pnt-14-94/Rab9^{S26N.UASp.YFP} is a non-suppressor of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{S26N.UASp.YFP} is a non-suppressor of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab9^{S26N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 partially rescues the bang sensitivity of para^bss1/+ flies, but does not rescue the bang sensitivity of hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

Expression of Rab9^{S26N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of either Scer\GAL4^Act5C.PU, Scer\GAL4^nSyb.PS or Scer\GAL4^C23 suppresses the reduced pupal length phenotype seen in Nf1^E2 animals.

(Walker et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23642

y¹ w^*; P{UASp-YFP.Rab9.S26N}04

23643

y¹ w^*; P{UASp-YFP.Rab9.S26N}crol¹¹/SM5; TM3, Sb¹/l(3)*^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab9^{S26N.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab9^{S26N.UASp.YFP}

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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