FB2025_01 , released February 20, 2025
Allele: Dmel\Rab11S25N.UASp.YFP
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General Information
Symbol
Dmel\Rab11S25N.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215411
Feature type
allele
Associated gene
Dmel\Rab11
Associated Insertion(s)
Carried in Construct
P{UASp-YFP.Rab11.S25N}
Also Known As
UASp-YFP.Rab11.S25N, Rab11 DN, DN-Rab11, UAS-YFP-Rab11DN, UAS-Rab11DN, Rab11DN, Rab11DN, UAS-Rab11-DN, UASp-YFP-Rab11S25N, UAS-Rab11DN
Key Links
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
in vitro construct
(Zhang et al., 2007, Zhang et al., 2007)
Progenitor genotype
Carried in construct
P{UASp-YFP.Rab11.S25N}
Cytology
Description

Amino acid replacement: S25N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab11 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Rab11
Tagged with
YFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  intestinal cancer
      CEA
      (Nie et al., 2019)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Charcot-Marie-Tooth disease type 4B1
      modeled by mtmG447D
      (Hu et al., 2022)
      DOES NOT ameliorate  Parkinson's disease
      modeled by Hsap\SNCAUAS.cKa
      (Breda et al., 2015)
      ameliorates  motor neuron disease
      modeled by scatHMS01910
      (Patel et al., 2020)
      exacerbates  motor neuron disease
      modeled by scatHMS01910
      (Wilkinson et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult ellipsoid body R-neuron, with Scer\GAL4Alp4-c507

      (Martin-Peña and Ferrus, 2020)

      border follicle cell, with Scer\GAL4c306

      (Miao et al., 2022)

      border follicle cell, with Scer\GAL4slbo.2.6

      (Chen et al., 2012, Assaker et al., 2010)

      chaeta, with Scer\GAL4Eq

      (Zhang et al., 2007)

      egg chamber, with Scer\GAL4VP16.mat.αTub67C

      (Catrina et al., 2016)

      egg chamber, with Scer\GAL4VP16.nanos.UTR, Scer\GAL4VP16.otu, Scer\GAL4nanos.PG

      (Catrina et al., 2016)

      ellipsoid body, with Scer\GAL4Alp4-c507

      (Martin-Peña and Ferrus, 2020)

      embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16AD.nrv2, Scer\GAL4DBD.wrapper.932i

      (Coutinho-Budd et al., 2017)

      embryonic/larval posterior spiracle | larval stage, with Scer\GAL4grh.D4

      (Wingen et al., 2017)

      embryonic/larval tracheal dorsal trunk | first instar larval stage, with Scer\GAL4grh.D4

      (Wingen et al., 2017)

      extracellular exosome, with Scer\GAL4dsx.KI

      (Corrigan et al., 2014)

      eye, with Scer\GAL4GMR.PF

      (Zhang et al., 2007)

      eye disc | larval stage | somatic clone, with Scer\GAL4Act5C.PP

      (Hu et al., 2022)

      filamentous actin, with Scer\GAL4VP16.nanos.UTR, Scer\GAL4VP16.otu, Scer\GAL4nanos.PG

      (Catrina et al., 2016)

      filamentous actin | oogenesis, with Scer\GAL4VP16.mat.αTub67C

      (Yu et al., 2024)

      interommatidial cell | P-stage | increased number | somatic clone, with Scer\GAL4Act5C.PP

      (Hu et al., 2022)

      larval tracheal system | embryonic stage, with Rab11N124I.UAS, Scer\GAL4btl.PS

      (Tsarouhas et al., 2023)

      male accessory gland secondary cell, with Scer\GAL4dsx.KI

      (Corrigan et al., 2014)

      mesonotum, with Scer\GAL4Eq

      (Zhang et al., 2007)

      ommatidium, with Scer\GAL4GMR.PF

      (Zhang et al., 2007)

      oocyte, with Scer\GAL4VP16.mat.αTub67C

      (Yu et al., 2024)

      rhabdomere | conditional, with Scer\GAL4ninaE.PT

      (Xiong et al., 2012)

      synaptic vesicle | third instar larval stage, with Scer\GAL4da.G32

      (Inoshita et al., 2017)

      tracheal lumen | embryonic stage, with Rab11N124I.UAS, Scer\GAL4btl.PS

      (Tsarouhas et al., 2023)

      wing, with Scer\GAL4bs-1348

      (Guichard et al., 2010)

      wing, with Scer\GAL4Bx-MS1096

      (Guichard et al., 2013, Guichard et al., 2010)

      wing hair | pupal stage, with Scer\GAL4Act5C.PI

      (Gault et al., 2012)

      wing vein, with Scer\GAL4bs-1348

      (Guichard et al., 2010)

      wing vein, with Scer\GAL4Bx-MS1096

      (Guichard et al., 2010)

      yolk granule | absent, with Scer\GAL4VP16.mat.αTub67C

      (Yu et al., 2024)
      Detailed Description
      Statement
      Reference

      1 day old adults expressing Rab11S25N.UASp.YFP under the control of Scer\GAL4futsch-C380 exhibit decreased climbing ability.

      (Wilkinson et al., 2021)

      Expression of Rab11S25N.UASp.YFP under the control of Scer\GAL4Alp4-c507 leads to a decrease in the axonal branching of the R-neurons in the ellipsoid body.

      (Martin-Peña and Ferrus, 2020)

      Expression of Rab11S25N.UASp.YFP under the control of CtxGlia-SplitGal4 (Hsim\VP16AD.nrv2 and Scer\GAL4DBD.wrapper.932i) leads to an altered cortex glial morphology in third instar larvae when compared to controls.

      (Coutinho-Budd et al., 2017)

      The third instar larval neuromuscular junction of individuals expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4da.G32 exhibits large presynaptic vesicles, as compared to controls.

      (Inoshita et al., 2017)

      Animals expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4grh.D4 are lethal, show defects in larval dorsal trunk elongation (resulting in internalization of posterior spiracles into the body cavity) and the larvae are smaller than age-matched controls.

      (Wingen et al., 2017)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not lead to any overt morphological defects in early stage egg chambers but beginning at stage 7 the oocyte development becomes delayed and in many late stage egg chambers the oocyte is detached from the posterior cortex but the F-actin network appears intact. When driven under the combined control of the Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 drivers both morphological and F-actin network defects are observed.

      (Catrina et al., 2016)

      Ectopic expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4C164 results in a decrease in the distance travelled per unit of time by third instar larvae in a crawling assay compared to controls.

      (Breda et al., 2015)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in secondary cells under the control of Scer\GAL4dsx.KI reduces exosome secretion into the accessory gland lumen compared to controls.

      Expression of ALiXGD7853 under the control of Scer\GAL4esg-NP7397 reduces the ability of males to prevent female remating. There are no significant effects on egg laying, fecundity or mating behavior.

      The nuclei of accessory gland secondary cells expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4esg-NP7397 are reduced in size compared compared to neighbouring main cell nuclei.

      (Corrigan et al., 2014)

      The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ are not significantly different from those seen in control hemocytes.

      (Garg and Wu, 2014)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of either Scer\GAL4eg-Mz360, Scer\GAL4eve.RN2 or Scer\GAL4elav-C155 does not result in any defects in axon outgrowth in the embryo.

      (van den Brink et al., 2013)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4slbo.2.6 impairs border cell migration in the developing egg chamber. No border cell cluster dissociation is seen.

      (Chen et al., 2012)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in somatic clones under the control of Scer\GAL4Act5C.PI results in cells with short, missing or malformed trichomes in pupal wings.

      (Gault et al., 2012)

      Flies expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4ninaE.PT which are raised at 18[o]C prior to eclosion and then raised under 12 hour light/12 hour dark conditions show a reduction in the amplitude of the electroretinogram at 3 weeks of age. Defects in rhabdomere morphology are also seen at this age. These retinal degeneration defects are not seen if the flies are raised in constant darkness after eclosion.

      (Xiong et al., 2012)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP driven by Scer\GAL4slbo.2.6 results in the impairment of border cell migration during egg chamber development.

      (Assaker et al., 2010)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in the wing disc primordium under the control of Scer\GAL4bs-1348 results in mild occasional notching of the wing margin and small wings with altered vein spacing.

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in the wing disc primordium under the control of Scer\GAL4Bx-MS1096 results in mild occasional notching of the wing margin and small wings with altered vein spacing.

      (Guichard et al., 2010)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4GMR.PF results in extensive fusion of ommatidia and cell death in the eye.

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Eq1 results in a strong loss of bristle phenotype and defects in the notum epithelium.

      (Zhang et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Adults co-expressing Rab11S25N.UASp.YFP and scatHMS01910 under the control of Scer\GAL4futsch-C380 show thorax muscle degeneration.

      (Wilkinson et al., 2021)

      Co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP with Rac1N17.Scer\UAS driven by Scer\GAL4slbo.2.6 enhances the border follicle cell migration phenotype associated with Rac1N17.Scer\UAS expression.

      Co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP with Rac1L89.Scer\UAS driven by Scer\GAL4slbo.2.6 enhances the border follicle cell migration phenotype associated with Rac1L89.Scer\UAS expression.

      (Assaker et al., 2010)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in Rho172O or Rho172F clones enhances the Rho1 mutant phenotype. In addition to frequent disruptions to adherens junctions between clonal cells, there is also disruption of adherens junctions between clonal and wild-type cells.

      (Warner and Longmore, 2009)
      Xenogenetic Interactions
      Statement
      Reference

      The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4C164 cannot be suppressed by co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP.

      The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4elav-C155 are further worsened by co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP.

      (Breda et al., 2015)

      Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in the wing disc primordium enhances the activity of Zzzz\EFScer\UAS.cGa (when both are under the control of Scer\GAL4Bx-MS1096).

      Co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP with Zzzz\lefScer\UAS.cGa in the wing disc primordium produces a strong and penetrant wing margin notching phenotype (when both are under the control of Scer\GAL4Bx-MS1096).

      (Guichard et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rab11DN
      (Lambert et al., 2022)
      Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP
      Rab11S25N.UASp.YFP
      Name Synonyms
      Secondary FlyBase IDs
        References (51)