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General Information
Symbol
Dmel\Rab11S25N.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215411
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Rab11 DN, Rab11DN, UAS-YFP-Rab11DN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: S25N.
UASp regulatory sequences drive expression of a predicted dominant negative form of Rab11 that is tagged at the N-terminal end with YFP.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The third instar larval neuromuscular junction of individuals expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4da.G32 exhibits large presynaptic vesicles, as compared to controls.
Animals expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4grh.D4 are lethal, show defects in larval dorsal trunk elongation (resulting in internalization of posterior spiracles into the body cavity) and the larvae are smaller than age-matched controls.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not lead to any overt morphological defects in early stage egg chambers but beginning at stage 7 the oocyte development becomes delayed and in many late stage egg chambers the oocyte is detached from the posterior cortex but the F-actin network appears intact. When driven under the combined control of the Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 drivers both morphological and F-actin network defects are observed.
Ectopic expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4C164 results in a decrease in the distance travelled per unit of time by third instar larvae in a crawling assay compared to controls.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in secondary cells under the control of Scer\GAL4dsx.KI reduces exosome secretion into the accessory gland lumen compared to controls. Expression of ALiXGD7853 under the control of Scer\GAL4esg-NP7397 reduces the ability of males to prevent female remating. There are no significant effects on egg laying, fecundity or mating behavior. The nuclei of accessory gland secondary cells expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4esg-NP7397 are reduced in size compared compared to neighbouring main cell nuclei.
The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ are not significantly different from those seen in control hemocytes.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of either Scer\GAL4eg-Mz360, Scer\GAL4eve.RN2 or Scer\GAL4elav-C155 does not result in any defects in axon outgrowth in the embryo.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4slbo.2.6 impairs border cell migration in the developing egg chamber. No border cell cluster dissociation is seen.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in somatic clones under the control of Scer\GAL4Act5C.PI results in cells with short, missing or malformed trichomes in pupal wings.
Flies expressing Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4ninaE.PT which are raised at 18[o]C prior to eclosion and then raised under 12 hour light/12 hour dark conditions show a reduction in the amplitude of the electroretinogram at 3 weeks of age. Defects in rhabdomere morphology are also seen at this age. These retinal degeneration defects are not seen if the flies are raised in constant darkness after eclosion.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP driven by Scer\GAL4slbo.2.6 results in the impairment of border cell migration during egg chamber development.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in the wing disc primordium under the control of Scer\GAL4bs-1348 results in mild occasional notching of the wing margin and small wings with altered vein spacing. Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in the wing disc primordium under the control of Scer\GAL4Bx-MS1096 results in mild occasional notching of the wing margin and small wings with altered vein spacing.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4GMR.PF results in extensive fusion of ommatidia and cell death in the eye. Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Eq1 results in a strong loss of bristle phenotype and defects in the notum epithelium.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Enhancer of
NOT Enhancer of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Enhancer of
NOT Enhancer of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP with Rac1N17.Scer\UAS driven by Scer\GAL4slbo.2.6 enhances the border follicle cell migration phenotype associated with Rac1N17.Scer\UAS expression. Co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP with Rac1L89.Scer\UAS driven by Scer\GAL4slbo.2.6 enhances the border follicle cell migration phenotype associated with Rac1L89.Scer\UAS expression.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in Rho172O or Rho172F clones enhances the Rho1 mutant phenotype. In addition to frequent disruptions to adherens junctions between clonal cells, there is also disruption of adherens junctions between clonal and wild-type cells.
Xenogenetic Interactions
Statement
Reference
The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4C164 cannot be suppressed by co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP. The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4elav-C155 are further worsened by co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP.
Expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP in the wing disc primordium enhances the activity of Zzzz\EFScer\UAS.cGa (when both are under the control of Scer\GAL4Bx-MS1096). Co-expression of Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP with Zzzz\lefScer\UAS.cGa in the wing disc primordium produces a strong and penetrant wing margin notching phenotype (when both are under the control of Scer\GAL4Bx-MS1096).
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Rab11S25N.Scer\UAS.P\T.T:Avic\GFP-YFP
Rab11S25N.UASp.YFP
Name Synonyms
Secondary FlyBase IDs
    References (28)