FlyBase Allele Report: Dmel\Rab11[Q70L.UASp.YFP]

General Information

Symbol

Dmel\Rab11^{Q70L.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215412

Feature type

allele

Associated gene

Dmel\Rab11

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab11.Q70L}

Also Known As

Rab11-CA, UAS-YFP-Rab11^CA, UAS-rab11^Q70L

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab11.Q70L}

Cytology

Description

Amino acid replacement: Q70L.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab11 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab11

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Charcot-Marie-Tooth disease type 4B1

modeled by mtm^G447D

(Hu et al., 2022)

ameliorates Parkinson's disease 2

modeled by park¹³

(Rai and Roy, 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Phenotype Manifest In

adult ellipsoid body R-neuron, with Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

ellipsoid body, with Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

wing hair | increased number, with Scer\GAL4^en-e16E

(Gault et al., 2012)

Detailed Description

Statement

Reference

Expression of Rab11^{Q70L.UASp.YFP} under the control of Scer\GAL4^Alp4-c507 leads to an increase in the axonal branching of the R-neurons (specifically in R2 neurons) in the ellipsoid body.

(Martin-Peña and Ferrus, 2020)

Expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} in the posterior compartment of the wing via Scer\GAL4^en-e16E at 29[o]C results in multiple trichomes.

(Gault et al., 2012)

Expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^ninaE.PT does not result in light-dependent retinal degeneration (flies raised under 12 hour light/12 our dark conditions show a normal electroretinogram amplitude at 3 weeks of age).

(Xiong et al., 2012)

Bristle morphogenesis is not affected by expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^sca.PU.

(Otani et al., 2011)

Expression via Scer\GAL4^pnr-MD237 results in flies with a wild type notum, apart from a single fly with cleft thorax.

(Thomas et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

NOT suppressed by

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor | partially of increased cell number | P-stage | somatic clone - tissue specific phenotype of ex^e1, mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor of increased size | somatic clone | adult stage phenotype of ex^e1, mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor of increased cell number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor | partially of increased cell number | P-stage | somatic clone - tissue specific phenotype of ft⁸, mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor of increased size | somatic clone | adult stage phenotype of ft⁸, mtm^null

(Hu et al., 2022)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^ninaE.PT is a suppressor | partially of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Scer\GAL4^ey.PH/Rab11^{Q70L.UASp.YFP} is a suppressor | partially of abnormal neurophysiology | somatic clone | adult stage | progressive | conditional phenotype of Crag^C

(Xiong et al., 2012)

NOT Suppressor of

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^NP0001 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Rab21^GD9402, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^NP0001 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Rab21^KK115582, Scer\GAL4^NP0001

(Nassari et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^en-e16E has wing hair | increased number phenotype, enhanceable by gish^GD10588, Scer\GAL4^en-e16E

(Gault et al., 2012)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^en-e16E has wing hair | increased number phenotype, enhanceable by gish[+]/gish^e01759

(Gault et al., 2012)

NOT Enhanced by

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507 has ellipsoid body phenotype, non-enhanceable by ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507 has adult ellipsoid body R-neuron phenotype, non-enhanceable by ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

NOT suppressed by

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507 has ellipsoid body phenotype, non-suppressible by ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507 has adult ellipsoid body R-neuron phenotype, non-suppressible by ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Enhancer of

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^sca.PU is an enhancer of scutellar bristle phenotype of IKKε^DN.UAS, Scer\GAL4^sca.PU

(Otani et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor | partially of interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype of ex^e1, mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor of mesonotum | somatic clone | adult stage phenotype of ex^e1, mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor of interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor | partially of eye disc | somatic clone | larval stage phenotype of mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor | partially of interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype of ft⁸, mtm^null

(Hu et al., 2022)

Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP} is a suppressor of mesonotum | somatic clone | adult stage phenotype of ft⁸, mtm^null

(Hu et al., 2022)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^ninaE.PT is a suppressor | partially of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^CCAP.PP is a suppressor | partially of wing phenotype of Osbp^UAS.cMa, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Scer\GAL4^ey.PH/Rab11^{Q70L.UASp.YFP} is a suppressor | partially of rhabdomere | somatic clone | progressive | conditional phenotype of Crag^C

(Xiong et al., 2012)

NOT Suppressor of

Statement

Reference

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^NP0001 is a non-suppressor of adult gut phenotype of Rab21^GD9402, Scer\GAL4^NP0001

(Nassari et al., 2022)

Rab11^{Q70L.UASp.YFP}, Scer\GAL4^NP0001 is a non-suppressor of adult gut phenotype of Rab21^KK115582, Scer\GAL4^NP0001

(Nassari et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

gish^e01759 dominantly enhances the wing hair phenotype resulting from the expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} via Scer\GAL4^en-e16E.

Co-expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} and gish^GD10588 via Scer\GAL4^en-e16E results in a strong enhancement of the wing hair phenotype compared with that resulting from the expression of either transgene alone.

(Gault et al., 2012)

Co-expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} weakly suppresses the uninflated wing phenotype of Scer\GAL4^Ccap.PP, Osbp^Scer\UAS.cMa flies.

(Ma et al., 2012)

The reduction in amplitude and in the size of the "on" and "off" transients of the electroretinogram that is seen in 3 week old flies containing homozygous Crag^C clones in the eye when they are raised under 12 hour light/12 hour dark conditions is partially suppressed by expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^ey.PH. Rhabdomere morphology is also better preserved in these animals compared to flies containing homozygous Crag^C clones in the eye in an otherwise wild-type background.

(Xiong et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

50783

P{UASp-YFP.Rab11.Q70L}20, y¹ w^*

9791

y¹ w^*; P{UASp-YFP.Rab11.Q70L}CG13895³¹

23260

y¹ w^*; P{UASp-YFP.Rab11.Q70L}Coop²⁴/SM5

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rab11^CA

(Lambert et al., 2022)

Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab11^{Q70L.UASp.YFP}

Rab11[Q70L]

(Otani et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (28)

Report Sections

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