FlyBase Allele Report: Dmel\Rab35[S22N.UAS.YFP]

General Information

Symbol

Dmel\Rab35^S22N.UAS.YFP

Species

D. melanogaster

Name

FlyBase ID

FBal0215441

Feature type

allele

Associated gene

Dmel\Rab35

Associated Insertion(s)

Carried in Construct

P{UAST-YFP.Rab35.S22N}

Also Known As

Rab35^DN, DN-Rab35

Key Links

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UAST-YFP.Rab35.S22N}

Cytology

Description

Amino acid replacement: S22N.

(Zhang et al., 2007)

UASt regulatory sequences drive expression of a predicted dominant negative form of Rab35 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rab35

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

abnormal neurophysiology, with Scer\GAL4^nSyb.PS

(Uytterhoeven et al., 2011)

partially lethal - majority die, with Scer\GAL4^Rab35.ATG

(Chan et al., 2011)

Phenotype Manifest In

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

embryonic/larval neuromuscular junction, with Scer\GAL4^nSyb.PS

(Uytterhoeven et al., 2011)

larval tracheal system | embryonic stage, with Scer\GAL4^btl.PS

(Tsarouhas et al., 2023)

tracheal lumen | embryonic stage, with Scer\GAL4^btl.PS

(Tsarouhas et al., 2023)

Detailed Description

Statement

Reference

Expression of Rab35^S22N.UAS.YFP under the control of CtxGlia-SplitGal4 (Hsim\VP16^AD.nrv2 and Scer\GAL4^{DBD.wrapper.932i}) leads to an altered cortex glial morphology in third instar larvae when compared to controls.

(Coutinho-Budd et al., 2017)

Scer\GAL4^He.PZ-driven expression of Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP} does not induce increase in the number of circulating hemocytes in third instar larvae relative to controls.

(Del Signore et al., 2017)

Expression of Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^btl.PS causes formation of ectopic gas-filled seamless tubes close to the terminal cell nucleus. Terminal cell actin cytoskeleton is not significantly altered.

(Schottenfeld-Roames and Ghabrial, 2012)

Excitatory junctional current (EJC) amplitude and quantal content at the neuromuscular junction are reduced in third instar larvae expressing Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^n-syb.PS compared to controls.

(Uytterhoeven et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^GMR57C10/Rab35^S22N.UAS.YFP is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^btl.PS/Rab35^S22N.UAS.YFP is a suppressor of lethal phenotype of wkd²²⁰/Df(3R)Exel6276

(Schottenfeld-Roames and Ghabrial, 2012)

Scer\GAL4^nSyb.PS/Rab35^S22N.UAS.YFP is a suppressor | partially of lethal phenotype of sky²

(Uytterhoeven et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR57C10/Rab35^S22N.UAS.YFP is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Other

Statement

Reference

Rab35^S22N.UAS.YFP, Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has abnormal cell shape phenotype

(Molina et al., 2022)

Arpc4^VDRC.cUa, Rab35^S22N.UAS.YFP, Scer\GAL4^Cg.PU has abnormal cell shape phenotype

(Molina et al., 2022)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^btl.PS/Rab35^S22N.UAS.YFP is a suppressor of terminal tracheal cell phenotype of wkd²²⁰/Df(3R)Exel6276

(Schottenfeld-Roames and Ghabrial, 2012)

Other

Statement

Reference

Rab11^N124I.UAS, Rab35^S22N.UAS.YFP, Scer\GAL4^btl.PS has tracheal lumen | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rab11^N124I.UAS, Rab35^S22N.UAS.YFP, Scer\GAL4^btl.PS has larval tracheal system | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rab35^S22N.UAS.YFP, Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has hemocyte phenotype

(Molina et al., 2022)

Arpc4^VDRC.cUa, Rab35^S22N.UAS.YFP, Scer\GAL4^Cg.PU has hemocyte phenotype

(Molina et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 partially rescues the bang sensitivity of para^bss1/+ flies, but does not rescue the bang sensitivity of hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

Expression of Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^btl.PS strongly rescues the "U-turn" phenotype seen in wkd²²⁰/Df(3R)Exel6276 mutant terminal tracheal cells. The pupal stage lethality seen in wkd²²⁰/Df(3R)Exel6276 mutants is also rescued.

(Schottenfeld-Roames and Ghabrial, 2012)

Expression of Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^n-syb.PS suppresses sky²/Df(2L)Exel7080 lethality; survival to the third larval instar is significantly improved and some of the double mutants eclose as uncoordinated adults.

(Uytterhoeven et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9819

y¹ w^*; P{UAST-YFP.Rab35.S22N}01

9820

y¹ w^*; P{UAST-YFP.Rab35.S22N}06

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rab35^DN

(Del Signore et al., 2017, Uytterhoeven et al., 2011)

Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP}

Rab35^S22N.UAS.YFP

Rab35^SN

(Del Signore et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

Open Close