FB2025_01 , released February 20, 2025
Allele: Dmel\ptcΔloop2.UAS
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General Information
Symbol
Dmel\ptcΔloop2.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0215576
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PtcΔloop2
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Carried in construct
Cytology
Description

UAS sequences drive expression of ptc lacking most of the second large extracellular loop.

The FRT cassette has been excised, allowing UAS sequences to drive expression of a mutated form of ptc that lacks most of the second extracellular loop (amino acids 738-939 are deleted and replaced by a VEV linker).

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ptcΔloop2.Scer\UAS under the control of Scer\GAL4GMR16A06 does not result in a pruning defect in the mushroom body gamma neurons.

31% of embryos expressing ptcΔloop2.Scer\UAS under the control of Scer\GAL4nos.PG have five or more "lost" germ cells that have failed to coalesce with the somatic gonadal precursor cells (less than 5% of wild-type embryos have this many "lost" germ cells).

When clones are made expressing ptcΔloop2.Scer\UAS (under the control of Scer\GAL4αTub84B.PC) in adult abdominal segments all clone cells that have anterior provenance, form pigmented (segmental region anterior 3 (a3)) cuticle. The polarity is largely reversed, even at the back of the clone and in some places behind it.

Expression of ptcΔloop2.Scer\UAS under the control of Scer\GAL41407 results in a decrease in the size of the brain in embryos. The frontal commissure is present.

Somatic clones of ptcΔloop2.Scer\UAS.cBa cells in the wings of Scer\GAL4Bx-MS1096 animals only cause a visible phenotype when they are located in the posterior portion of the anterior (A) compartment, next to the anterior/posterior (P) boundary. Such clones are associated with the formation of mirror-symmetric anterior winglets in place of the normal A compartment and with a dramatic reduction in the P compartment. The anterior border of such clones coincides with the plane of symmetry of the anterior winglet. In larvae, clones of cells expressing ptcΔloop2.Scer\UAS.cBa under the control of Scer\GAL4αTub84B.PP in the posterior of the anterior compartment of wing discs have a smooth, straight anterior border.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ptcΔloop2.Scer\UAS.cBa
ptcΔloop2.Scer\UAS
ptcΔloop2.UAS
Name Synonyms
Secondary FlyBase IDs
  • FBal0138170
  • FBal0150291
References (11)