Allele Dmel\lid^{JmjC.mut.Scer\UAS.P\T}

General Information
Symbol	Dmel\lid^{JmjC.mut.Scer\UAS.P\T}	Species	D. melanogaster
Name		FlyBase ID	FBal0215596
Feature type	allele	Associated gene	Dmel\lid

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Secombe et al., 2007) in vitro construct - regulatory fusion (Secombe et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of full-length lid containing two point mutations in the demethylase domain. (Secombe et al., 2007) Amino acid replacement: H637A. (Secombe et al., 2007) Amino acid replacement: E639A. (Secombe et al., 2007)
Carried in construct	P{UASp-lid.JmjC.mut} (Secombe et al., 2007, Li et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile, with Scer\GAL4^Act.PU (Li et al., 2010) viable, with Scer\GAL4^Act.PU (Li et al., 2010)
Phenotype Manifest In

Detailed Description
	Statement Reference Females expressing lid[JmjC.mut.Scer\UAS.P\T] under the control of Scer\GAL4[Act.PU] do not lay eggs. (Li et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference lid^{JmjC.mut.Scer\UAS.P\T}, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dm^Scer\UAS.cZa (Secombe et al., 2007, Li et al., 2010)
Phenotype Manifest In
Enhancer of
Statement Reference lid^{JmjC.mut.Scer\UAS.P\T}, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dm^Scer\UAS.cZa (Secombe et al., 2007, Li et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of lid^{JmjC.mut.Scer\UAS.P\T} enhances the rough eye phenotype caused by expression of dm^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF. (Secombe et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	Scer\GAL4^Act.PU/lid^{JmjC.mut.Scer\UAS.P\T} fails to rescue lid¹⁰⁴²⁴ (Li et al., 2010)
Comments	Expression of lid[JmjC.mut.Scer\UAS.P\T] under the control of Scer\GAL4[Act.PU] does not rescue the lethality of lid[10424] homozygotes. (Li et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	lid^{JmjC.mut.Scer\UAS.P\T}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Li et al., 2010, PLoS Genet. 6(11): e1001221 Essential functions of the histone demethylase lid. [FBrf0212448] Secombe et al., 2007, Genes Dev. 21(5): 537--551 The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth. [FBrf0193047]

Allele Dmel\lidJmjC.mut.Scer\UAS.P\T

Allele Dmel\lid^{JmjC.mut.Scer\UAS.P\T}