Allele Dmel\Gr64a^Scer\UAS.cJa

General Information
Symbol	Dmel\Gr64a^Scer\UAS.cJa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Jiao	FlyBase ID	FBal0215609
Feature type	allele	Associated gene	Dmel\Gr64a

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Jiao et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Gr64a coding sequences. (Jiao et al., 2007)
Carried in construct	P{UAS-Gr64a.J} (Jiao et al., 2007, Jiao et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Expression of Gr64a[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] does not restore the trehalose-response in Df(3L)ΔGr64 mutants. Expression of Gr64a[Scer\UAS.cJa] in Df(3L)ΔGr64 mutants under the control of Scer\GAL4[Gr5a.1] does not restore the behavioural and electrophysiological responses to any of the sugars sucrose, maltose and glucose. Df(3L)ΔGr64 mutants expressing both Gr64f[Scer\UAS.cJa] and Gr64a[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] show a behavioural preference for sucrose over fructose that is very similar to that of wild-type flies. The wild-type frequency of action potentials in response to sucrose is also restored in these animals. Expression of Gr64a[Scer\UAS.cJa] in Df(3L)ΔGr64 mutants under the control of Scer\GAL4[Gr5a.1] do not improve their behavioural and electrophysiological responses to glucose. Co-expression of Gr64a[Scer\UAS.cJa] and Gr64f[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] restores normal preference for glucose over fructose and wild-type frequencies of glucose dependent actin potentials. (Jiao et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Scer\GAL4^Gr5a.8.5/Gr64a^Scer\UAS.cJa partially rescues Df(3L)Gr64^ab (Jiao et al., 2007)
Comments	Expression of Gr64a[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.8.5] in Df(3L)Gr64[ab] flies fully restores the preferences for sucrose, glucose, or maltose over fructose, but not the trehalose response. Consistent with this, the reduction of action potentials in response to glucose, sucrose, and maltose seen in Df(3L)Gr64[ab] flies is rescued through expression of Gr64a[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.8.5]. However, the reduction of action potentials in response to trehalose is not rescued. (Jiao et al., 2007)
Stocks ( 1 )
Bloomington	25080 w^*; P{UAS-Gr64a.J}2/CyO; TM6B, Tb¹/MKRS
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Gr64a^Scer\UAS.cJa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Jiao
Secondary FlyBase IDs

References ( 2 )
Research paper	Jiao et al., 2008, Curr. Biol. 18(22): 1797--1801 Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila. [FBrf0206328] Jiao et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(35): 14110--14115 A Drosophila gustatory receptor required for the responses to sucrose, glucose, and maltose identified by mRNA tagging. [FBrf0200681]

Allele Dmel\Gr64aScer\UAS.cJa

Allele Dmel\Gr64a^Scer\UAS.cJa