Allele Dmel\Dscam^3.31.8

General Information
Symbol	Dmel\Dscam^3.31.8	Species	D. melanogaster
Name		FlyBase ID	FBal0215824
Feature type	allele	Associated gene	Dmel\Dscam

Allele class
Mutagen	recombination, FLPase, gene targeting by homologous recombination
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	FLPase (Hattori et al., 2007) gene targeting by homologous recombination (Hattori et al., 2007) recombination (Hattori et al., 2007, Hattori et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Dp(2;2)Dscam^5'HR-3.31.8 (Hattori et al., 2007) Dp(2;2)Dscam^ED69 (Hattori et al., 2007)
Nature of the lesion	Statement Reference Recombination between the single Scer\FRT sites that are present on each of Dp(2;2)Dscam^ED69 and Dp(2;2)Dscam^5'HR-3.31.8 has resulted a single copy of Dscam in which the genomic region encoding exons 3 to 16 has been replaced with cDNA sequences. This copy of Dscam encodes a single ectodomain isoform, containing the variable exons 4.3, 6.31 and 9.8. A single Scer\FRT site is present following the cDNA sequences. (Hattori et al., 2007) Homologous recombination has been used to replace the genomic region encoding the variable ectodomains with cDNA sequences encoding a single isoform, which in this case contains the variable exons 4.3, 6.31 and 9.8. A single Scer\FRT site is inserted in the intron between exons 16 and 17. (Hattori et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage (with Dscam^6.5.9) (Hattori et al., 2007) lethal \| larval stage (with Dscam^10.27.25) (Hattori et al., 2007) lethal \| larval stage \| recessive (Hattori et al., 2007) neuroanatomy defective \| dominant (Hattori et al., 2007) neuroanatomy defective \| embryonic stage (Hattori et al., 2007) neuroanatomy defective \| embryonic stage (with Dscam^6.5.9) (Hattori et al., 2007) neuroanatomy defective \| embryonic stage (with Dscam^10.27.25) (Hattori et al., 2007) neuroanatomy defective \| embryonic stage (with Dscam²³) (Hattori et al., 2007) neuroanatomy defective \| pupal stage (Hattori et al., 2007)
Phenotype Manifest In
	antennal lobe (Hattori et al., 2007) antennal lobe glomerulus (Hattori et al., 2007) lobe system of mushroom body (Hattori et al., 2007) longitudinal connective (Hattori et al., 2007) longitudinal connective (with Dscam^6.5.9) (Hattori et al., 2007) longitudinal connective (with Dscam^10.27.25) (Hattori et al., 2007) longitudinal connective (with Dscam²³) (Hattori et al., 2007) olfactory receptor neuron (Hattori et al., 2007) presumptive embryonic/larval central nervous system (Hattori et al., 2007) presumptive embryonic/larval central nervous system (with Dscam^6.5.9) (Hattori et al., 2007) presumptive embryonic/larval central nervous system (with Dscam^10.27.25) (Hattori et al., 2007) presumptive embryonic/larval central nervous system (with Dscam²³) (Hattori et al., 2007)
Detailed Description
	Statement Reference Homozygotes, Dscam^10.27.25/Dscam^3.31.8 and Dscam^3.31.8/Dscam^6.5.9 animals die in early larval development. Over 75% of Dscam^3.31.8/Dscam²³ animals survive to late pupal stages. Homozygous, Dscam^10.27.25/Dscam^3.31.8 and Dscam^3.31.8/Dscam^6.5.9 embryos show defects in the organisation of the central nervous system, showing severely disrupted longitudinal tracts and aberrant midline crossing. Dscam^3.31.8/Dscam²³ embryos show defects in the organisation of the central nervous system, showing disrupted longitudinal tracts. Dscam^3.31.8/Dscam^- animals have a highly disorganised antennal lobe with no distinct glomerular structure and olfactory receptor neurons form many ectopic termini throughout the antennal lobe. Almost all Dscam^3.31.8/Dscam^- mushroom bodies completely lack one lobe (typically the dorsal lobe is missing). Dscam^3.31.8/+ animals also show defects in the mushroom body in approximately 90% of cases; either one mushroom body lobe is absent (approximately 60% of cases) or one mushroom body lobe is thinner than the other (approximately 30% of cases). Sister branches of single Dscam^3.31.8 mushroom body neurons in a Dscam^FRT control background (generated using intragenic MARCM) segregate into the two mushroom body lobes with high fidelity. (Hattori et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Dscam^3.31.8 (Hattori et al., 2007, Hattori et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Hattori et al., 2007, Nature 449(7159): 223--227 Dscam diversity is essential for neuronal wiring and self-recognition. [FBrf0205027]
Supplementary material	Hattori et al., 2007, Nature 449(7159): Supplementary Figure. [FBrf0204464]

Allele Dmel\Dscam3.31.8

Allele Dmel\Dscam^3.31.8