|Feature type||allele||Associated gene||Dmel\Dscam|
|Mutagen||recombination, FLPase, gene targeting by homologous recombination|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Recombination between the single Scer\FRT sites that are present on each of Dp(2;2)DscamED69 and Dp(2;2)Dscam5'HR-3.31.8 has resulted a single copy of Dscam in which the genomic region encoding exons 3 to 16 has been replaced with cDNA sequences. This copy of Dscam encodes a single ectodomain isoform, containing the variable exons 4.3, 6.31 and 9.8. A single Scer\FRT site is present following the cDNA sequences.
Homologous recombination has been used to replace the genomic region encoding the variable ectodomains with cDNA sequences encoding a single isoform, which in this case contains the variable exons 4.3, 6.31 and 9.8. A single Scer\FRT site is inserted in the intron between exons 16 and 17.
|Phenotype Manifest In|
Homozygotes, Dscam10.27.25/Dscam3.31.8 and Dscam3.31.8/Dscam6.5.9 animals die in early larval development. Over 75% of Dscam3.31.8/Dscam23 animals survive to late pupal stages. Homozygous, Dscam10.27.25/Dscam3.31.8 and Dscam3.31.8/Dscam6.5.9 embryos show defects in the organisation of the central nervous system, showing severely disrupted longitudinal tracts and aberrant midline crossing. Dscam3.31.8/Dscam23 embryos show defects in the organisation of the central nervous system, showing disrupted longitudinal tracts. Dscam3.31.8/Dscam- animals have a highly disorganised antennal lobe with no distinct glomerular structure and olfactory receptor neurons form many ectopic termini throughout the antennal lobe. Almost all Dscam3.31.8/Dscam- mushroom bodies completely lack one lobe (typically the dorsal lobe is missing). Dscam3.31.8/+ animals also show defects in the mushroom body in approximately 90% of cases; either one mushroom body lobe is absent (approximately 60% of cases) or one mushroom body lobe is thinner than the other (approximately 30% of cases). Sister branches of single Dscam3.31.8 mushroom body neurons in a DscamFRT control background (generated using intragenic MARCM) segregate into the two mushroom body lobes with high fidelity.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 2 )|