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General Information
Symbol
Dmel\rictorΔ2
Species
D. melanogaster
Name
FlyBase ID
FBal0215849
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference
Imprecise excision of the P{EPgy2}rictorEY08986 insertion, resulting in a deletion that removes rictor exons 2-4 and part of exon 5.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Homozygous neurons (generated via a Scer\FLP1SOP / MARCM method) show a complicated phenotype that combines features of both undergrowth and miniaturization.
Mutant embryos have a normal somatic muscle pattern. The number of nuclei in the segmental border muscle is normal.
Homozygotes show a dendritic tiling defect in class IV dendrite arborization (da) neurons, with 8.1 +/- 1.7% of dendritic branches crossing one another. In addition, the total number of dendrite branches is reduced to approximately 80% of wild type. Single-cell dorsal class IV da neuron clones that are homozygous for rictorΔ2 (in a heterozygous background) show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type. The v'ada and vdaB dendrites often invade neighbouring dendritic fields in homozygous mutants, in contrast to what is seen in wild-type controls.
rictorΔ1/rictorΔ2 flies are normal in appearance. rictorΔ2 flies raised under controlled conditions on a rich diet show a modest (approximately 10%) reduction in body weight and a modest reduction in tissue growth (wing area is reduced) compared to controls. rictorΔ1 flies raised on nutrient-reduced food show growth that is indistinguishable from that of wild-type controls raised under the same conditions.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
rictorΔ2-homozygous or heterozygous pupae show a significant decrease in weight upon the expression of Tl10b.UAS.cUa under the control of Scer\GAL4r4; there is also apparently smaller pupae only in the rictorΔ2-homozygous background.
The level of non-contacting dendrite crossing in class IV dendrite arborizing larval neurons is significantly increased in rictorΔ2;Df(2R)Sema-2b-C4 double mutants compared to either rictorΔ2/+ or Df(2R)Sema-2b-C4/+ heterozygotes.
Scer\GAL4109(2)80-mediated overexpression of CHORDScer\UAS.cSa in rictorΔ2 mutant neurons partially restores the features of the miniature phenotype, but not those of the undergrowth phenotype.
The abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is enhanced by rictorΔ2/Y. The mitochondrial aggregation phenotype seen in the muscles of flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is enhanced by rictorΔ2. The ability of rictorΔ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is blocked by co-expression of either MarfmiRNA.cUa.Scer\UAS or Atg1Scer\UAS.cSa. The ability of rictorΔ2 to enhance the abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is partially suppressed by co-expression of MiroKK102189.
The axon guidance phenotypes of RhebScer\UAS.cPa, Scer\GAL4elav.PLu flies are not significantly rescued in a rictorΔ1/rictorΔ2 background. The RhebScer\UAS.cPa, Scer\GAL4elav.PLu synapse overgrowth phenotype is significantly rescued in a rictorΔ1/rictorΔ2 background.
rictorΔ2/+ ; Sin1e03756/+ double heterozygotes have a dendritic tiling defect in class IV dendrite arborization (da) neurons, with a significantly higher number of dendritic branches crossing one another compared to wild type (neither single heterozygote shows this phenotype). rictorΔ2 ; Sin1e03756 double homozygotes show dendritic tiling defects in class IV da neurons that are indistinguishable from the single homozygotes. The dendritic branches of ddaC neurons in TorΔP/+ ; rictorΔ2/+ double heterozygous larvae show increased crossing over of each other, indicating a tiling defect. Class IV da neurons of rictorΔ2/+ ; trc1/+ double heterozygous larvae show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type. Expression of trcScer\UAS.cUa under the control of Scer\GAL4ppk.PG slightly reduces the dendritic tiling defects seen in ddaC neurons in rictorΔ2 animals. Expression of trcScer\UAS.T:Myr1 under the control of Scer\GAL4ppk.PG significantly rescues the dendritic tiling defects seen in ddaC neurons in rictorΔ2 animals. Expression of trcT449A.Scer\UAS.T:Myr1 under the control of Scer\GAL4ppk.PG does not rescue the dendritic tiling defects seen in ddaC neurons in rictorΔ2 animals.
The severity of the rough eye phenotype caused by expression of foxoScer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PF is enhanced by rictorΔ2 at both 18 and 25oC. The overgrowth of the salivary gland that is seen larvae expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4nub-AC-62 is suppressed by rictorΔ2. rictorΔ2 suppresses the overgrowth of the eye caused by homozygous Pten3 clones. rictorΔ2 does not suppress the overgrowth of the eye caused by homozygous Tsc1R453X clones.
Xenogenetic Interactions
Statement
Reference
Expression of Hsap\PARK2Scer\UAS.cYa completely blocks the ability of rictorΔ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.
Complementation and Rescue Data
Comments
Class IV neuron-specific expression of rictorScer\UAS.cHa largely rescues the dendritic phenotypes of single-cell rictorΔ2 class IV dendrite arborization neuron clones. Expression of rictorScer\UAS.cHa under the control of Scer\GAL4ppk.PG largely rescues the tiling defects seen in v'ada and vdaB neurons of rictorΔ2 mutants.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (8)