Allele Dmel\rictor^Δ2

General Information
Symbol	Dmel\rictor^Δ2	Species	D. melanogaster
Name		FlyBase ID	FBal0215849
Feature type	allele	Associated gene	Dmel\rictor

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity (Hietakangas and Cohen, 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EPgy2}rictor^EY08986 (Hietakangas and Cohen, 2007)
Nature of the lesion	Statement Reference Imprecise excision of the P{EPgy2}rictor^EY08986 insertion, resulting in a deletion that removes rictor exons 2-4 and part of exon 5. (Hietakangas and Cohen, 2007)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Hietakangas and Cohen, 2007) fertile (with rictor^Δ1) (Hietakangas and Cohen, 2007) neuroanatomy defective (Koike-Kumagai et al., 2009) neuroanatomy defective \| cell autonomous \| somatic clone (Koike-Kumagai et al., 2009) small body (Hietakangas and Cohen, 2007) viable (Hietakangas and Cohen, 2007) viable (with rictor^Δ1) (Hietakangas and Cohen, 2007)
Phenotype Manifest In
	abdominal anterior ventral multidendritic neuron vdaa (Koike-Kumagai et al., 2009) class IV dendritic arborizing neuron (Koike-Kumagai et al., 2009) class IV dendritic arborizing neuron \| cell autonomous \| somatic clone (Koike-Kumagai et al., 2009) dendrite (Koike-Kumagai et al., 2009) dendrite \| cell autonomous \| somatic clone (Koike-Kumagai et al., 2009) dorsal multidendritic neuron ddaC (Koike-Kumagai et al., 2009) ventral multidendritic neuron vdaB (Koike-Kumagai et al., 2009) wing (Hietakangas and Cohen, 2007)
Detailed Description
	Statement Reference Homozygotes show a dendritic tiling defect in class IV dendrite arborization (da) neurons, with 8.1 +/- 1.7% of dendritic branches crossing one another. In addition, the total number of dendrite branches is reduced to approximately 80% of wild type. Single-cell dorsal class IV da neuron clones that are homozygous for rictor[Δ2] (in a heterozygous background) show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type. The v'ada and vdaB dendrites often invade neighbouring dendritic fields in homozygous mutants, in contrast to what is seen in wild-type controls. (Koike-Kumagai et al., 2009) rictor^Δ1/rictor^Δ2 flies are normal in appearance. rictor^Δ2 flies raised under controlled conditions on a rich diet show a modest (approximately 10%) reduction in body weight and a modest reduction in tissue growth (wing area is reduced) compared to controls. rictor^Δ1 flies raised on nutrient-reduced food show growth that is indistinguishable from that of wild-type controls raised under the same conditions. (Hietakangas and Cohen, 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference rictor^Δ2 has neuroanatomy defective phenotype, suppressible \| partially by trc^Scer\UAS.cUa/Scer\GAL4^ppk.PG (Koike-Kumagai et al., 2009) rictor^Δ2 has neuroanatomy defective phenotype, suppressible by Scer\GAL4^ppk.PG/trc^{Scer\UAS.T:Myr-Src64B} (Koike-Kumagai et al., 2009)
NOT suppressed by
Statement Reference rictor^Δ2 has neuroanatomy defective phenotype, non-suppressible by trc^{T449A.Scer\UAS.T:Myr-Src64B}/Scer\GAL4^ppk.PG (Koike-Kumagai et al., 2009)
Enhancer of
Statement Reference rictor^Δ2 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, foxo^{Scer\UAS.T:Avic\GFP} (Hietakangas and Cohen, 2007)
Suppressor of
Statement Reference rictor^Δ2 is a suppressor of hyperplasia \| somatic clone phenotype of Pten³ (Hietakangas and Cohen, 2007) rictor^Δ2 is a suppressor of hyperplasia phenotype of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^nub-AC-62 (Hietakangas and Cohen, 2007)
NOT Suppressor of
Statement Reference rictor^Δ2/rictor^Δ1 is a non-suppressor of neuroanatomy defective phenotype of Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) rictor^Δ2 is a non-suppressor of hyperplasia \| somatic clone phenotype of Tsc1^R453X (Hietakangas and Cohen, 2007)
Other
Statement Reference rictor^Δ2, trc¹/trc[+] has neuroanatomy defective phenotype (Koike-Kumagai et al., 2009) Sin1[+]/Sin1^e03756, rictor^Δ2 has neuroanatomy defective \| dominant phenotype (Koike-Kumagai et al., 2009) Tor^ΔP, rictor[+]/rictor^Δ2 has neuroanatomy defective \| dominant phenotype (Koike-Kumagai et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference rictor^Δ2 has dorsal multidendritic neuron ddaC phenotype, suppressible \| partially by trc^Scer\UAS.cUa/Scer\GAL4^ppk.PG (Koike-Kumagai et al., 2009) rictor^Δ2 has dorsal multidendritic neuron ddaC phenotype, suppressible by Scer\GAL4^ppk.PG/trc^{Scer\UAS.T:Myr-Src64B} (Koike-Kumagai et al., 2009)
NOT suppressed by
Statement Reference rictor^Δ2 has dorsal multidendritic neuron ddaC phenotype, non-suppressible by trc^{T449A.Scer\UAS.T:Myr-Src64B}/Scer\GAL4^ppk.PG (Koike-Kumagai et al., 2009)
Enhancer of
Statement Reference rictor^Δ2 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, foxo^{Scer\UAS.T:Avic\GFP} (Hietakangas and Cohen, 2007)
Suppressor of
Statement Reference rictor^Δ2/rictor^Δ1 is a suppressor of embryonic/larval neuromuscular junction phenotype of Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) rictor^Δ2 is a suppressor of embryonic/larval salivary gland phenotype of Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^nub-AC-62 (Hietakangas and Cohen, 2007) rictor^Δ2 is a suppressor of eye \| somatic clone phenotype of Pten³ (Hietakangas and Cohen, 2007)
NOT Suppressor of
Statement Reference rictor^Δ2/rictor^Δ1 is a non-suppressor of photoreceptor cell R7 phenotype of Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) rictor^Δ2/rictor^Δ1 is a non-suppressor of photoreceptor cell R8 phenotype of Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) rictor^Δ2 is a non-suppressor of eye \| somatic clone phenotype of Tsc1^R453X (Hietakangas and Cohen, 2007)
Other
Statement Reference rictor^Δ2, trc¹/trc[+] has class IV dendritic arborizing neuron phenotype (Koike-Kumagai et al., 2009) rictor^Δ2, trc¹/trc[+] has dendrite phenotype (Koike-Kumagai et al., 2009) Sin1[+]/Sin1^e03756, rictor^Δ2 has class IV dendritic arborizing neuron phenotype (Koike-Kumagai et al., 2009) Sin1[+]/Sin1^e03756, rictor^Δ2 has dendrite phenotype (Koike-Kumagai et al., 2009) Tor^ΔP, rictor[+]/rictor^Δ2 has dendrite phenotype (Koike-Kumagai et al., 2009) Tor^ΔP, rictor[+]/rictor^Δ2 has dorsal multidendritic neuron ddaC phenotype (Koike-Kumagai et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference The axon guidance phenotypes of Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] flies are not significantly rescued in a rictor[Δ1]/rictor[Δ2] background. The Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] synapse overgrowth phenotype is significantly rescued in a rictor[Δ1]/rictor[Δ2] background. (Dimitroff et al., 2012) rictor[Δ2]/+ ; Sin1[e03756]/+ double heterozygotes have a dendritic tiling defect in class IV dendrite arborization (da) neurons, with a significantly higher number of dendritic branches crossing one another compared to wild type (neither single heterozygote shows this phenotype). rictor[Δ2] ; Sin1[e03756] double homozygotes show dendritic tiling defects in class IV da neurons that are indistinguishable from the single homozygotes. The dendritic branches of ddaC neurons in Tor[ΔP]/+ ; rictor[Δ2]/+ double heterozygous larvae show increased crossing over of each other, indicating a tiling defect. Class IV da neurons of rictor[Δ2]/+ ; trc[1]/+ double heterozygous larvae show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type. Expression of trc[Scer\UAS.cUa] under the control of Scer\GAL4[ppk.PG] slightly reduces the dendritic tiling defects seen in ddaC neurons in rictor[Δ2] animals. Expression of trc[Scer\UAS.T:Myr1] under the control of Scer\GAL4[ppk.PG] significantly rescues the dendritic tiling defects seen in ddaC neurons in rictor[Δ2] animals. Expression of trc[T449A.Scer\UAS.T:Myr1] under the control of Scer\GAL4[ppk.PG] does not rescue the dendritic tiling defects seen in ddaC neurons in rictor[Δ2] animals. (Koike-Kumagai et al., 2009) The severity of the rough eye phenotype caused by expression of foxo^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF is enhanced by rictor^Δ2 at both 18 and 25^oC. The overgrowth of the salivary gland that is seen larvae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nub-AC-62 is suppressed by rictor^Δ2. rictor^Δ2 suppresses the overgrowth of the eye caused by homozygous Pten³ clones. rictor^Δ2 does not suppress the overgrowth of the eye caused by homozygous Tsc1^R453X clones. (Hietakangas and Cohen, 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	rictor^Δ2 is rescued by rictor^Scer\UAS.cHa/Scer\GAL4^ppk.PG (Koike-Kumagai et al., 2009) rictor^Δ2 is rescued by rictor^Scer\UAS.cHa/Scer\GAL4^tub.PU (Hietakangas and Cohen, 2007) rictor^Δ2 is rescued by rictor^Scer\UAS.cHa/Scer\GAL4^unspecified (Koike-Kumagai et al., 2009)
Comments	Class IV neuron-specific expression of rictor[Scer\UAS.cHa] largely rescues the dendritic phenotypes of single-cell rictor[Δ2] class IV dendrite arborization neuron clones. Expression of rictor[Scer\UAS.cHa] under the control of Scer\GAL4[ppk.PG] largely rescues the tiling defects seen in v'ada and vdaB neurons of rictor[Δ2] mutants. (Koike-Kumagai et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	rictor^Δ2 (Hietakangas and Cohen, 2007, Koike-Kumagai et al., 2009, Dimitroff et al., 2012) rictor D2 (Hietakangas and Cohen, 2007)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Dimitroff et al., 2012, PLoS ONE 7(2): e30722 Diet and Energy-Sensing Inputs Affect TorC1-Mediated Axon Misrouting but Not TorC2-Directed Synapse Growth in a Drosophila Model of Tuberous Sclerosis. [FBrf0217461] Koike-Kumagai et al., 2009, EMBO J. 28(24): 3879--3892 The target of rapamycin complex 2 controls dendritic tiling of Drosophila sensory neurons through the Tricornered kinase signalling pathway. [FBrf0209531] Hietakangas and Cohen, 2007, Genes Dev. 21(6): 632--637 Re-evaluating AKT regulation: role of TOR complex 2 in tissue growth. [FBrf0201456]

Allele Dmel\rictorΔ2

Allele Dmel\rictor^Δ2