Allele Dmel\hiw^{Scer\UAS.W.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\hiw^{Scer\UAS.W.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0215863
Feature type	allele	Associated gene	Dmel\hiw

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Wu et al., 2005) in vitro construct - regulatory fusion (Wu et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of full-length hiw coding sequences (15.7kb) downstream of T:Avic\GFP^EGFP sequences. (Wu et al., 2005)
Carried in construct	P{UAS-GFP-hiw} (Wu et al., 2005, Tian et al., 2011, Xiong et al., 2010, Shen and Ganetzky, 2009)
Tagged with	T:Avic\GFP^EGFP (Wu et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference Scer\GAL4^elav-C155/hiw^{Scer\UAS.W.T:Avic\GFP-EGFP} is a non-suppressor of NMJ bouton phenotype of Rae1^EX28 (Tian et al., 2011) Scer\GAL4^elav-C155/hiw^{Scer\UAS.W.T:Avic\GFP-EGFP} is a non-suppressor of synapse phenotype of Rae1^EX28 (Tian et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Presynaptic expression of hiw[Scer\UAS.W.T:Avic\GFP-EGFP] under the control of Scer\GAL4[elav-C155] fails to rescue the bouton number increase found in Rae1[EX28] mutant synapses. (Tian et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^{elav.Switch.PO}/hiw^{Scer\UAS.W.T:Avic\GFP-EGFP} rescues hiw^ND8 (Wu et al., 2005)
Comments	Expression of hiw^{Scer\UAS.T:Avic\GFP-EGFP} throughout development, under the control of Scer\GAL4^{elav.Switch.PO}, results in a robust rescue of the hiw^ND8/Y morphological phenotype with an approximate 77% reduction in the number of boutons, along with robust rescue of quantal content. In the absence of RU486, which activates transcription of Scer\GAL4^{elav.Switch.PO}, and therefore hiw^{Scer\UAS.T:Avic\GFP-EGFP}, hiw^ND8/Y synapses are still extremely overgrown; however, there is apparently some leaky expression because there is a 27% decrease in the number of synaptic boutons. Leaky expression leads to a nearly complete rescue of the quantal content, indicating that very low dose of hiw are effective in rescuing synaptic function. When RU486 is added during larval development, there is an additional approximately 25% reduction in bouton number, compared with hiw^ND8/Y larvae in the absence of RU486. Compared with leaky expression, late expression of hiw^{Scer\UAS.T:Avic\GFP-EGFP} does not lead to a significant decrease in synaptic branching or synaptic expansion in hiw^ND8/Y mutants. There is a complete rescue of synaptic function, with a significant increase in quantal content compared to leaky hiw^{Scer\UAS.T:Avic\GFP-EGFP} expression in the absence of RU486. (Wu et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	hiw^{Scer\UAS.W.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Tian et al., 2011, Nat. Neurosci. 14(10): 1267--1275 Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons. [FBrf0216253] Xiong et al., 2010, J. Cell Biol. 191(1): 211--223 Protein turnover of the Wallenda/DLK kinase regulates a retrograde response to axonal injury. [FBrf0211939] Shen and Ganetzky, 2009, J. Cell Biol. 187(1): 71--79 Autophagy promotes synapse development in Drosophila. [FBrf0208921] Wu et al., 2005, J. Neurosci. 25(42): 9557--9566 Highwire function at the Drosophila neuromuscular junction: spatial, structural, and temporal requirements. [FBrf0191056]

Allele Dmel\hiwScer\UAS.W.T:Avic\GFP-EGFP

Allele Dmel\hiw^{Scer\UAS.W.T:Avic\GFP-EGFP}