Scer\GAL4elav-C155/hiwUAS.W.EGFP is a non-suppressor of synapse phenotype of Rae1EX28
Scer\GAL4elav-C155/hiwUAS.W.EGFP is a non-suppressor of NMJ bouton phenotype of Rae1EX28
Presynaptic expression of hiwScer\UAS.W.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 fails to rescue the bouton number increase found in Rae1EX28 mutant synapses.
Expression of hiwScer\UAS.W.T:Avic\GFP-EGFP under the control of Scer\GAL4VGlut-OK371 suppresses the strong protective effect of hiw052 on L1 vein neurons following axotomy. As in wild type, the severed axons undergo fragmentation.
Expression of hiwScer\UAS.T:Avic\GFP-EGFP throughout development, under the control of Scer\GAL4elav.Switch.PO, results in a robust rescue of the hiwND8/Y morphological phenotype with an approximate 77% reduction in the number of boutons, along with robust rescue of quantal content.
In the absence of RU486, which activates transcription of Scer\GAL4elav.Switch.PO, and therefore hiwScer\UAS.T:Avic\GFP-EGFP, hiwND8/Y synapses are still extremely overgrown; however, there is apparently some leaky expression because there is a 27% decrease in the number of synaptic boutons. Leaky expression leads to a nearly complete rescue of the quantal content, indicating that very low dose of hiw are effective in rescuing synaptic function.
When RU486 is added during larval development, there is an additional approximately 25% reduction in bouton number, compared with hiwND8/Y larvae in the absence of RU486. Compared with leaky expression, late expression of hiwScer\UAS.T:Avic\GFP-EGFP does not lead to a significant decrease in synaptic branching or synaptic expansion in hiwND8/Y mutants. There is a complete rescue of synaptic function, with a significant increase in quantal content compared to leaky hiwScer\UAS.T:Avic\GFP-EGFP expression in the absence of RU486.
