FB2025_01 , released February 20, 2025
Allele: Dmel\hiwΔRING.UAS.Tag:TAP
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General Information
Symbol
Dmel\hiwΔRING.UAS.Tag:TAP
Species
D. melanogaster
Name
FlyBase ID
FBal0215868
Feature type
allele
Associated gene
Dmel\hiw
Associated Insertion(s)
Carried in Construct
P{UAS-TAP-hiwΔRING}
Also Known As
UAS-NTAP-hiwΔRING
Key Links
Transgenic product class
missense_variant
(Wu et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wu et al., 2005)
Mutagen
in vitro construct
(Wu et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-TAP-hiwΔRING}
Cytology
Description

Amino acid replacement: C4991S.

(Wu et al., 2005)

UAS regulatory sequences drive expression of hiw coding sequence in which the first two cysteine residues (C4991 and C4994) in the RING domain are mutagenised to serine, with a Tag:TAP tag at the N-terminus.

(Wu et al., 2005)

Amino acid replacement: C4994S.

(Wu et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
hiw
Tagged with
Tag:TAP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of hiwΔRING.Scer\UAS.T:Zzzz\TAP through the strong neuronal driver Scer\GAL4elav.Switch.PO results in a more than twofold increase in both the number of boutons and the number of branchpoints, a 50% increase in synaptic span and a 50% reduction in bouton size.

      Expression of hiwΔRING.Scer\UAS.T:Zzzz\TAP under the control of Scer\GAL4elav.Switch.PO in a hiwND8 heterozygous background leads to an additional 56% increase in the number of boutons and an additional 42% increase in the number of branch points, compared with the expression of hiwΔRING.Scer\UAS in a wild-type background.

      (Wu et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      hiwΔRING.UAS.Tag:TAP/Scer\GAL4elav-C155 fails to rescue hiwND8

      (Tian et al., 2011)

      hiwΔRING.UAS.Tag:TAP/Scer\GAL4elav.PLu fails to rescue hiwND8

      (Wu et al., 2005)
      Comments

      Expression of hiwΔRING.Scer\UAS.T:Zzzz\TAP under the control of Scer\GAL4elav.PLu is unable to rescue either the quantal size or quantal content defects of hiwND8 hemizygotes.

      (Wu et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      hiwΔRING.Scer\UAS.T:Zzzz\TAP
      hiwΔRING.UAS.Tag:TAP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)