mir-8Δ2/Df(2R)BSC382 males show a significant increase in median lifespan compared to controls.
3-5 day old mir-8Δ2/Df(2R)mir-8Δ3 adults show reduced climbing ability in a negative geotaxis assay.
mir-8Δ2/Df(2R)mir-8Δ3 adults show significant defects in the hemolymph-brain barrier (as determined using a dye injection assay).
Homozygous larvae show a significant reduction in evoked excitatory junctional potential (EJP) amplitude at the neuromuscular junction compared to controls. Amplitude of the spontaneous miniature EJP (mEJP) is normal, but mEJP frequency and quantal content are reduced.
The neuroepithelium of mir-8Δ2 L3 larvae is 143% overgrown compared to wild type. In the subsequent stage, ectopic and precocious neuroblasts are scattered throughout this enlarged neuroepithelium.
Total bouton number at the neuromuscular junction is significantly decreased compared to controls in mir-8Δ1/mir-8Δ2 larvae.
Wing size is reduced by 10% compared to controls in mir-8Δ2/Df(2R)mir-8Δ3 adults, and the wings have extra veins.
Mutant male and female adults are significantly smaller in size and mass than wild-type controls. Mutant adults have a reduced wing size which is caused by reduced wing cell number with no significant change in cell size.
Mutant larvae have a significantly smaller body volume than wild-type larvae at 100 hours after egg laying. The onset of pupariation occurs at the normal time, however, there is a slight delay in adult emergence in the mutant animals.
Cells of homozygous clones in the fat body are smaller than the adjacent wild-type twin-spot cells. When homozygous clones are induced in the fat body at the embryonic or newly hatched larval stage, few or no homozygous cells are seen next to the wild-type twin spots, suggesting a failure of proliferation and survival of the mutant cells during larval development. Homozygous clones in the wing or eye disc have little growth defects.
Mutants show no defects in targeting of the DL1 glomerulus in the antennal lobe.
mir-8Δ1/mir-8Δ2 and mir-8Δ2/Df(2R)mir-8Δ3 animals show reduced survival during pupal and early adult stages, either failing to emerge from the pupal case or dying within the first 24 hours of eclosion.
mir-8Δ1/mir-8Δ2 and mir-8Δ2/Df(2R)mir-8Δ3 adult escapers often have wing defects (the wings are not properly unfolded) or leg defects.
mir-8Δ2/Df(2R)mir-8Δ3 third instar larvae show increased levels of apoptosis in the brain compared to controls.
3 day old mir-8Δ2/Df(2R)mir-8Δ3 adults perform less well in a climbing assay than controls, and the severity of the defect is increased in 9 day old flies.