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General Information
Symbol
Dmel\mir-8Δ2
Species
D. melanogaster
Name
FlyBase ID
FBal0216287
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EP}EP2269 insertion, resulting in a 1.8kb deletion that removes the mir-8 gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mir-8Δ2/Df(2R)BSC382 males show a significant increase in median lifespan compared to controls.

3-5 day old mir-8Δ2/Df(2R)mir-8Δ3 adults show reduced climbing ability in a negative geotaxis assay.

mir-8Δ2/Df(2R)mir-8Δ3 adults show significant defects in the hemolymph-brain barrier (as determined using a dye injection assay).

Homozygous larvae show a significant reduction in evoked excitatory junctional potential (EJP) amplitude at the neuromuscular junction compared to controls. Amplitude of the spontaneous miniature EJP (mEJP) is normal, but mEJP frequency and quantal content are reduced.

The neuroepithelium of mir-8Δ2 L3 larvae is 143% overgrown compared to wild type. In the subsequent stage, ectopic and precocious neuroblasts are scattered throughout this enlarged neuroepithelium.

Total bouton number at the neuromuscular junction is significantly decreased compared to controls in mir-8Δ1/mir-8Δ2 larvae.

Wing size is reduced by 10% compared to controls in mir-8Δ2/Df(2R)mir-8Δ3 adults, and the wings have extra veins.

Mutant male and female adults are significantly smaller in size and mass than wild-type controls. Mutant adults have a reduced wing size which is caused by reduced wing cell number with no significant change in cell size.

Mutant larvae have a significantly smaller body volume than wild-type larvae at 100 hours after egg laying. The onset of pupariation occurs at the normal time, however, there is a slight delay in adult emergence in the mutant animals.

Cells of homozygous clones in the fat body are smaller than the adjacent wild-type twin-spot cells. When homozygous clones are induced in the fat body at the embryonic or newly hatched larval stage, few or no homozygous cells are seen next to the wild-type twin spots, suggesting a failure of proliferation and survival of the mutant cells during larval development. Homozygous clones in the wing or eye disc have little growth defects.

Homozygous mir-8Δ2 mutants show a malformed adult third leg phenotype. No defects are observed in early leg disc patterning and morphogenesis. Neuromuscular junction defects are seen in third instar larvae.

A mir-8Δ2/+ background enhances the malformed third leg phenotype seen when mir-8sponge.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4αTub84B.PL.

A mir-8Δ2/+ background enhances the third instar larval neuromuscular junction phenotypes seen when mir-8sponge.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4αTub84B.PL.

No detectable neuromuscular junction phenotypes are seen when mir-8sponge.Scer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4elav.PLu in a mir-8Δ2/+ background.

Flies expressing mir-8sponge.Scer\UAS.T:Avic\GFP-EGFP in a mir-8Δ2 background under the control of the leg specific driver Scer\GAL4Dll.PU appear indistinguishable from controls.

Mutants show no defects in targeting of the DL1 glomerulus in the antennal lobe.

mir-8Δ1/mir-8Δ2 and mir-8Δ2/Df(2R)mir-8Δ3 animals show reduced survival during pupal and early adult stages, either failing to emerge from the pupal case or dying within the first 24 hours of eclosion.

mir-8Δ1/mir-8Δ2 and mir-8Δ2/Df(2R)mir-8Δ3 adult escapers often have wing defects (the wings are not properly unfolded) or leg defects.

mir-8Δ2/Df(2R)mir-8Δ3 third instar larvae show increased levels of apoptosis in the brain compared to controls.

3 day old mir-8Δ2/Df(2R)mir-8Δ3 adults perform less well in a climbing assay than controls, and the severity of the defect is increased in 9 day old flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

SerRX106/+ fully rescues the defects in wing size and partially suppresses the extra wing vein phenotype caused by mir-8Δ2/Df(2R)mir-8Δ3.

Expression of ushVDRC.cUa under the control of Scer\GAL4Cg.PA significantly rescues the reduced weight of mir-8Δ2 adults.

ush1513/+ significantly rescues the reduced weight of mir-8Δ2 adults.

One copy of ena210 partially suppresses the neuromuscular junction phenotypes seen in mir-8Δ2 homozygous mutants.

The survival defect of mir-8Δ2/Df(2R)mir-8Δ3 animals is partially suppressed by Gug35/+. The proportion of surviving adults that have leg defects is also reduced in the double mutants and the leg malformations are less severe than those seen in mir-8Δ2/Df(2R)mir-8Δ3 single mutants.

The impairment in climbing ability that is seen in 3 and 9 day old mir-8Δ2/Df(2R)mir-8Δ3 adults is less severe if the flies also carry Gug35/+.

Xenogenetic Interactions
Statement
Reference

Expression of P{UAS-mir-200-c} (which encodes Hsap\MIR200CScer\UAS.cHa and Hsap\MIR141Scer\UAS.cHa) under the control of either Scer\GAL4Cg.PA or Scer\GAL4ppl.PP rescues the reduced weight of mir-8Δ2 adults to near that of wild type.

Expression of Zzzz\actAFP4mito.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4how-24B rescues the neuromuscular junction phenotypes seen in mir-8Δ2 larvae.

The survival defect of mir-8Δ2/Df(2R)mir-8Δ3 animals is partially suppressed by low-level expression of BacA\p35Scer\UAS.cHa (in the absence of a Scer\GAL4 driver).

Complementation and Rescue Data
Not rescued by
Comments

Expression of mir-8Scer\UAS.cHa under the control of either Scer\GAL4Cg.PA or Scer\GAL4ppl.PP rescues the reduced weight of mir-8Δ2 adults to near that of wild type.

Expression of mir-8Scer\UAS.cHa under the control of Scer\GAL4elav.PU does not rescue the reduced weight of mir-8Δ2 adults.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (11)