FlyBase Allele Report: Dmel\mir-8[Δ2]

Homozygous larvae show a significant reduction in evoked excitatory junctional potential (EJP) amplitude at the neuromuscular junction compared to controls. Amplitude of the spontaneous miniature EJP (mEJP) is normal, but mEJP frequency and quantal content are reduced.

(Loya et al., 2014)

The neuroepithelium of mir-8^Δ2 L3 larvae is 143% overgrown compared to wild type. In the subsequent stage, ectopic and precocious neuroblasts are scattered throughout this enlarged neuroepithelium.

(Morante et al., 2013)

Total bouton number at the neuromuscular junction is significantly decreased compared to controls in mir-8^Δ1/mir-8^Δ2 larvae.

(Nesler et al., 2013)

Wing size is reduced by 10% compared to controls in mir-8^Δ2/Df(2R)mir-8^Δ3 adults, and the wings have extra veins.

(Vallejo et al., 2011)

Mutant male and female adults are significantly smaller in size and mass than wild-type controls. Mutant adults have a reduced wing size which is caused by reduced wing cell number with no significant change in cell size.

Mutant larvae have a significantly smaller body volume than wild-type larvae at 100 hours after egg laying. The onset of pupariation occurs at the normal time, however, there is a slight delay in adult emergence in the mutant animals.

Cells of homozygous clones in the fat body are smaller than the adjacent wild-type twin-spot cells. When homozygous clones are induced in the fat body at the embryonic or newly hatched larval stage, few or no homozygous cells are seen next to the wild-type twin spots, suggesting a failure of proliferation and survival of the mutant cells during larval development. Homozygous clones in the wing or eye disc have little growth defects.

(Hyun et al., 2009)

Homozygous mir-8^Δ2 mutants show a malformed adult third leg phenotype. No defects are observed in early leg disc patterning and morphogenesis. Neuromuscular junction defects are seen in third instar larvae.

A mir-8^Δ2/+ background enhances the malformed third leg phenotype seen when mir-8^{sponge.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^αTub84B.PL.

A mir-8^Δ2/+ background enhances the third instar larval neuromuscular junction phenotypes seen when mir-8^{sponge.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^αTub84B.PL.

No detectable neuromuscular junction phenotypes are seen when mir-8^{sponge.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^elav.PLu in a mir-8^Δ2/+ background.

Flies expressing mir-8^{sponge.Scer\UAS.T:Avic\GFP-EGFP} in a mir-8^Δ2 background under the control of the leg specific driver Scer\GAL4^Dll.PU appear indistinguishable from controls.

(Loya et al., 2009)

Mutants show no defects in targeting of the DL1 glomerulus in the antennal lobe.

(Berdnik et al., 2008)

mir-8^Δ1/mir-8^Δ2 and mir-8^Δ2/Df(2R)mir-8^Δ3 animals show reduced survival during pupal and early adult stages, either failing to emerge from the pupal case or dying within the first 24 hours of eclosion.

mir-8^Δ1/mir-8^Δ2 and mir-8^Δ2/Df(2R)mir-8^Δ3 adult escapers often have wing defects (the wings are not properly unfolded) or leg defects.

mir-8^Δ2/Df(2R)mir-8^Δ3 third instar larvae show increased levels of apoptosis in the brain compared to controls.

3 day old mir-8^Δ2/Df(2R)mir-8^Δ3 adults perform less well in a climbing assay than controls, and the severity of the defect is increased in 9 day old flies.

(Karres et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-8^Δ2/Df(2R)mir-8^Δ3 has visible phenotype, suppressible | partially by Ser[+]/Ser^RX106

(Vallejo et al., 2011)

mir-8^Δ2/Df(2R)mir-8^Δ3 has abnormal size phenotype, suppressible by Ser[+]/Ser^RX106

(Vallejo et al., 2011)

mir-8^Δ2 has decreased body size phenotype, suppressible by Scer\GAL4^Cg.PA/ush^VDRC.cUa

(Hyun et al., 2009)

mir-8^Δ2 has decreased body size phenotype, suppressible by ush[+]/ush¹⁵¹³

(Hyun et al., 2009)

mir-8^Δ2 has decreased body size phenotype, suppressible by Hsap\MIR200C^UAS.cHa/Scer\GAL4^Cg.PA/Hsap\MIR141^UAS.cHa

(Hyun et al., 2009)

mir-8^Δ2 has decreased body size phenotype, suppressible by Hsap\MIR200C^UAS.cHa/Scer\GAL4^ppl.PP/Hsap\MIR141^UAS.cHa

(Hyun et al., 2009)

mir-8^Δ2 has abnormal neuroanatomy phenotype, suppressible | partially by ena[+]/ena²¹⁰

(Loya et al., 2009)

mir-8^Δ2 has abnormal neuroanatomy phenotype, suppressible by Lmon\actA^{FP4mito.UAS.EGFP}/Scer\GAL4^how-24B

(Loya et al., 2009)

mir-8^Δ2/Df(2R)mir-8^Δ3 has abnormal locomotor behavior | adult stage phenotype, suppressible by Gug³⁵/Gug[+]

(Karres et al., 2007)

mir-8^Δ2/Df(2R)mir-8^Δ3 has partially lethal phenotype, suppressible | partially by Gug³⁵/Gug[+]

(Karres et al., 2007)

mir-8^Δ2/Df(2R)mir-8^Δ3 has partially lethal phenotype, suppressible | partially by BacA\p35^UAS.cHa

(Karres et al., 2007)

Other

Statement

Reference

miPEP-8^alt/miPEP-8[+], mir-8^Δ2 has visible | adult stage phenotype

(Montigny et al., 2021)

miPEP-8^alt/miPEP-8[+], mir-8^Δ2 has decreased size | adult stage phenotype

(Montigny et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

mir-8^Δ2/Df(2R)mir-8^Δ3 has wing vein | ectopic phenotype, suppressible | partially by Ser[+]/Ser^RX106

(Vallejo et al., 2011)

mir-8^Δ2/Df(2R)mir-8^Δ3 has wing phenotype, suppressible by Ser[+]/Ser^RX106

(Vallejo et al., 2011)

mir-8^Δ2 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by ena[+]/ena²¹⁰

(Loya et al., 2009)

mir-8^Δ2 has NMJ bouton | larval stage phenotype, suppressible | partially by ena[+]/ena²¹⁰

(Loya et al., 2009)

mir-8^Δ2 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Lmon\actA^{FP4mito.UAS.EGFP}/Scer\GAL4^how-24B

(Loya et al., 2009)

mir-8^Δ2 has NMJ bouton | larval stage phenotype, suppressible by Lmon\actA^{FP4mito.UAS.EGFP}/Scer\GAL4^how-24B

(Loya et al., 2009)

mir-8^Δ2/Df(2R)mir-8^Δ3 has leg phenotype, suppressible | partially by Gug³⁵/Gug[+]

(Karres et al., 2007)

Other

Statement

Reference

miPEP-8^alt/miPEP-8[+], mir-8^Δ2 has wing phenotype

(Montigny et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

miPEP-8^alt/+, mir-8^Δ2/+ individuals show a small wing.

(Montigny et al., 2021)

Ser^RX106/+ fully rescues the defects in wing size and partially suppresses the extra wing vein phenotype caused by mir-8^Δ2/Df(2R)mir-8^Δ3.

(Vallejo et al., 2011)

Expression of ush^VDRC.cUa under the control of Scer\GAL4^Cg.PA significantly rescues the reduced weight of mir-8^Δ2 adults.

ush¹⁵¹³/+ significantly rescues the reduced weight of mir-8^Δ2 adults.

(Hyun et al., 2009)

One copy of ena²¹⁰ partially suppresses the neuromuscular junction phenotypes seen in mir-8^Δ2 homozygous mutants.

(Loya et al., 2009)

The survival defect of mir-8^Δ2/Df(2R)mir-8^Δ3 animals is partially suppressed by Gug³⁵/+. The proportion of surviving adults that have leg defects is also reduced in the double mutants and the leg malformations are less severe than those seen in mir-8^Δ2/Df(2R)mir-8^Δ3 single mutants.

The impairment in climbing ability that is seen in 3 and 9 day old mir-8^Δ2/Df(2R)mir-8^Δ3 adults is less severe if the flies also carry Gug³⁵/+.

(Karres et al., 2007)

Xenogenetic Interactions

Statement

Reference

Expression of P{UAS-mir-200-c} (which encodes Hsap\MIR200C^Scer\UAS.cHa and Hsap\MIR141^Scer\UAS.cHa) under the control of either Scer\GAL4^Cg.PA or Scer\GAL4^ppl.PP rescues the reduced weight of mir-8^Δ2 adults to near that of wild type.

(Hyun et al., 2009)

Expression of Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^how-24B rescues the neuromuscular junction phenotypes seen in mir-8^Δ2 larvae.

(Loya et al., 2009)

The survival defect of mir-8^Δ2/Df(2R)mir-8^Δ3 animals is partially suppressed by low-level expression of BacA\p35^Scer\UAS.cHa (in the absence of a Scer\GAL4 driver).

(Karres et al., 2007)

Complementation and Rescue Data

Rescued by

mir-8^Δ2 is rescued by mir-8^UAS.cHa/Scer\GAL4^Cg.PA

(Hyun et al., 2009)

mir-8^Δ2 is rescued by mir-8^UAS.cHa/Scer\GAL4^ppl.PP

(Hyun et al., 2009)

Not rescued by

mir-8^Δ2 is not rescued by mir-8^UAS.cHa/Scer\GAL4^elav.PU

(Hyun et al., 2009)

Comments

Expression of mir-8^Scer\UAS.cHa under the control of either Scer\GAL4^Cg.PA or Scer\GAL4^ppl.PP rescues the reduced weight of mir-8^Δ2 adults to near that of wild type.

Expression of mir-8^Scer\UAS.cHa under the control of Scer\GAL4^elav.PU does not rescue the reduced weight of mir-8^Δ2 adults.

(Hyun et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58932

w^*; mir-8^Δ2/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

miR-8^Δ2

(Loya et al., 2014, Lu et al., 2014, Nesler et al., 2013)

miR8^Δ2

(Loya et al., 2009)

miR8^Δ

(Loya et al., 2009)

mir-8^Δ2

(Guo et al., 2020, Antonello et al., 2015, Morante et al., 2013, Vallejo et al., 2011, Hyun et al., 2009, Karres et al., 2007)

mir8²

(Loya et al., 2009)

Δ2

(Montigny et al., 2021, Chen et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (13)

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