FlyBase Allele Report: Dmel\sei[HP21840]

General Information

Symbol

Dmel\sei^HP21840

Species

D. melanogaster

Name

FlyBase ID

FBal0216619

Feature type

allele

Associated gene

Dmel\sei

Associated Insertion(s)

P{EPg}sei^HP21840

Carried in Construct

Also Known As

seiP

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EPg}sei^HP21840

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPg}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:24,051,424..24,051,424 [+]

(Matthews, 2008.4.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of heart conduction disease

CEA

(Hill et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception | adult stage

(Senthilan et al., 2012)

abnormal developmental rate

(Dermady et al., 2024)

abnormal developmental rate (with sei^trunc)

(Dermady et al., 2024)

abnormal heat stress response | adult stage | recessive

(Hill et al., 2019)

abnormal locomotor behavior | larval stage

(Hill et al., 2019)

abnormal locomotor behavior | larval stage | heat sensitive

(Zheng et al., 2014)

abnormal neuroanatomy | larval stage

(Hill et al., 2019)

abnormal neurophysiology | adult stage

(Senthilan et al., 2012)

abnormal neurophysiology | larval stage | heat sensitive

(Zheng et al., 2014)

chemical resistant | adult stage

(Hill et al., 2019)

increased body weight | female

(Dermady et al., 2024)

increased rate of movement | embryonic stage

(Dermady et al., 2024)

paralytic | adult stage | heat sensitive

(Zheng et al., 2014)

Phenotype Manifest In

embryonic/larval neuromuscular junction

(Hill et al., 2019)

motor neuron | larval stage | heat sensitive

(Zheng et al., 2014)

Detailed Description

Statement

Reference

sei^HP21840/sei^HP21840 mutant adults have a significantly reduced threshold to heat-induced seizures, compared to controls, but significantly increased survival during hydrogen peroxide exposure; mutant adults have no differences in the number of neuromuscular junction branches or boutons, compared to controls.

Homozygous sei^HP21840/sei^HP21840 larvae have significant alterations in locomotion behaviors at 37[o]C, but no effect on the distance they travel at 25[o]C or 13[o]C, compared to controls; they have significantly faster responses to being touched with a heated probe than controls; mutant larvae have significantly increased neuromuscular junction branches, compared to controls, with no effect on the number of boutons.

(Hill et al., 2019)

Mutant flies show more rapid paralysis in response to acute heat stress than wild-type controls.

At 38[o]C, motor neurons become hyper-excitable in mutant larvae. The larvae show increased abnormal seizure-like locomotion at this temperature compared to wild-type controls.

(Zheng et al., 2014)

Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. Nonlinear mechanical amplification is significantly reduced compared to wild type.

(Senthilan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sei^HP21840 is rescued by Scer\GAL4^elav-C155/sei^3'UTR.UAS

(Zheng et al., 2014)

sei^HP21840 is rescued by sei^Δ3'UTR.UAS/Scer\GAL4^elav-C155

(Zheng et al., 2014)

Not rescued by

sei^HP21840 is not rescued by Scer\GAL4^elav-C155/Avic\GFP^{EGFP.sei.3'UTR.UAS}

(Zheng et al., 2014)

Comments

The decreased time to total paralysis in response to heat stress which is seen in sei^HP21840 flies is fully rescued by expression of either sei^{3'UTR.Scer\UAS} or sei^{Δ3'UTR.Scer\UAS} under the control of Scer\GAL4^elav-C155.

The decreased time to total paralysis in response to heat stress which is seen in sei^HP21840 flies is not rescued by expression of Avic\GFP^E.sei.3'UTR under the control of Scer\GAL4^elav-C155.

(Zheng et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

21935

w¹¹¹⁸; P{EPg}sei^HP21840

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sei^HP21840

(Kanellopoulos et al., 2020, Senthilan et al., 2012, Matthews, 2008.4.15)

sei^P

(Zheng et al., 2014)

seiP

(Dermady et al., 2024, Hill et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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