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General Information
Symbol
Dmel\PDZ-GEF4
Species
D. melanogaster
Name
FlyBase ID
FBal0216662
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A deletion of about 900 bp in the 5' promoter sequence of the Gef26 gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eye and wing phenotypes (small rough eyes, abnormal wings) and some degree of lethality are seen in various PDZ-GEF allelic combinations, with their relative strength in the following order: PDZ-GEF4/PDZ-GEF6 = PDZ-GEF4/PDZ-GEF5 > PDZ-GEF3/PDZ-GEF4 > PDZ-GEFk13720/PDZ-GEF3 = PDZ-GEFk13720/PDZ-GEF4 > PDZ-GEFEP388/PDZ-GEF3 > PDZ-GEFk13720/PDZ-GEFk13720 > PDZ-GEFk13720/PDZ-GEFEP388 > PDZ-GEFEP388/PDZ-GEFEP388.

Many mutant females have three spermathecae instead of two, with different allelic combinations having different percentages of females with three spermathecae: PDZ-GEF3/PDZ-GEF3 22%, PDZ-GEF3/PDZ-GEF4 30%, PDZ-GEF4/PDZ-GEF5 59%, PDZ-GEF4/PDZ-GEF6 62%, PDZ-GEFk13720/PDZ-GEF4 % (all PDZ-GEFk13720/PDZ-GEF4, PDZ-GEFk13720/PDZ-GEF3 or PDZ-GEFEP388/PDZ-GEF3 females have two spermathecae).

The number of male germline stem cells is dramatically reduced in the testes of Gef263/Gef264 animals compared to wild-type. In Gef264/Gef265 or Gef264/Gef266 the reduction is even more dramatic, with some testes lacking germ cells altogether. The secondary spermatogonia (identifiable by their branched fusomes) move to the tip.

In Gef263/Gef264 testes, the hub is not lost, but expression of some marker genes in the hub is. The number of cyst progenitor cells is also reduced in these testes.

During larval stages, male germline stem cells in Gef263/Gef264 animals become detached from the hub. Marker localisation data suggests that the adherens junctions between the hub cells and the surrounding stem cells are impaired.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Suppressed by
Statement
Reference

PDZ-GEF3/PDZ-GEF4 has cell-cell adherens junction & hub cell phenotype, suppressible by shgUAS.sgGFP/Scer\GAL4upd1.PU

PDZ-GEF6/PDZ-GEF4 has cell-cell adherens junction & hub cell phenotype, suppressible by Rap1V12.UAS.Tag:MYC/Scer\GAL4upd1.PU

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of shgScer\UAS.T:Avic\GFP-rs driven by Scer\GAL4da.PU almost fully suppresses the supernumerary spermathecae phenotype (as well as wing and eye phenotypes) in PDZ-GEF4/PDZ-GEF3 flies.

PDZ-GEF3/PDZ-GEF4 has no effect on the male germline stem cell over-proliferation phenotype due to upd1Scer\UAS.cCa; Scer\GAL4nos.UTR.T:Hsim\VP16.

The reduction in germline stem cells seen in the testes of PDZ-GEF3/PDZ-GEF4 animals is significantly suppressed by Egfrf24/+.

RV12.Scer\UAS.T:Hsap\MYC; Scer\GAL4upd1.PU rescues the formation of adherens junctions between male germline stem cells and hub cells in the germinal proliferation center of PDZ-GEF4/PDZ-GEF6 animals. The same phenotype in PDZ-GEF4/PDZ-GEF3 animals is rescued by shgScer\UAS.T:Avic\GFP-rs; Scer\GAL4upd1.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

PDZ-GEF+t11.6 rescues eye and wing phenotypes of PDZ-GEF3/PDZ-GEF4, PDZ-GEF4/PDZ-GEF5 or PDZ-GEF4/PDZ-GEF6 flies. PDZ-GEF+t11.6 partially rescues the supernumerary spermathecae phenotype in PDZ-GEF4/PDZ-GEF5 flies.

Germline stem cell loss in the testes of Gef264/Gef266 animals is rescued by Gef26+t11.6.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PDZ-GEF4
Name Synonyms
Secondary FlyBase IDs
    References (2)