FB2025_01 , released February 20, 2025
Allele: Dmel\Atg1K38Q.UAS
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General Information
Symbol
Dmel\Atg1K38Q.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0216675
Feature type
allele
Associated gene
Dmel\Atg1
Associated Insertion(s)
Carried in Construct
P{UAS-Atg1.K38Q}
Also Known As
UAS-Atg1K38Q, Atg1K38Q, UAS-Atg1DN, UAS-Atg1KQ
Key Links
Transgenic product class
dominant_negative_variant
(Berry and Baehrecke, 2007)
missense_variant
(Scott et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Berry and Baehrecke, 2007)
missense_variant
(Scott et al., 2007)
Mutagen
in vitro construct
(Scott et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-Atg1.K38Q}
Cytology
Description

UAS regulatory sequences drive expression of a dominant-negative form of Atg1.

(Berry and Baehrecke, 2007)

Amino acid replacement: K38Q.

(Scott et al., 2007)

UASt regulatory sequences drive expression of a mutated form of Atg1.

(Scott et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Atg1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  dilated cardiomyopathy 1A
      modeled by LamCG489V.UAS
      (Bhide et al., 2018)
      exacerbates  Parkinson's disease 6
      modeled by Pink1RNAi.UAS.cYa
      (Liu and Lu, 2010)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN has no effect on neuroblast size or number in late pupal stages.

      (Homem et al., 2014)

      Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Sgs3.PD blocks the formation of the large acidic structures which normally occurs in salivary gland cells approximately 1.5 hours after head eversion, but caspase activation at 2 hours after head eversion is not blocked. This does not prevent breakdown of the salivary gland tissue, as no persistent salivary gland phenotype is seen at 24 hours after puparium formation.

      (Ihry and Bashirullah, 2014)

      Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Lsp2.PH results in 25% pupal lethality.

      (Liu et al., 2013)

      At 24 hours after puparium formation, animals expressing Atg1K38Q.Scer\UAS under the control of Scer\GAL4fkh.PH still contain vacuolated salivary gland fragments, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.

      (Berry and Baehrecke, 2007)

      The partial lethality caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4GMR.PF can be partially suppressed by co-expression of Atg1K38Q.Scer\UAS. The reduced eye size caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is rescued by co-expression of Atg1K38Q.Scer\UAS.

      (Scott et al., 2007)

      Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Cg.PA does not result in increased autophagy in the fat body of normally fed animals.

      Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Act5C.PP in clones in the wing disc does not result in cell elimination or DNA fragmentation.

      Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Act5C.PP in clones or under the control of Scer\GAL4Cg.PA is sufficient to inhibit starvation-induced autophagy.

      (Scott et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The robust formation of Atg8a-positive (or LysoTracker positive) autophagosome vesicles the AdukScer\UAS.cBa-expressing (controlled by Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) fat body clones in fed larvae is not observed when the clones co-express Atg1K38Q.Scer\UAS.

      (Braden and Neufeld, 2016)

      Simultaneous overexpression of Atg1K38Q.Scer\UAS and NcΔN.C-A.Scer\UAS in the remodelling fat body, under the control of Scer\GAL4Lsp2.PH dramatically reduces autophagy and cell death but increases lethality by approximately 40%.

      Simultaneous overexpression of Atg1K38Q.Scer\UAS and NcΔN.C-A.Scer\UAS in the remodelling fat body, under the control of Scer\GAL4Lsp2.PH delays pupal development by approximately 4 hours.

      (Liu et al., 2013)

      Neuroblasts are detected in the mushroom body of 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) and Atg1K38Q.Scer\UAS under the control of Scer\GAL4wor.PA (mushroom body neuroblasts are not seen in wild-type adults).

      (Siegrist et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4hs.PH/Atg1K38Q.UAS partially rescues Atg1Δ3D

      (Scott et al., 2007)
      Comments

      Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4hs.PH in the absence of heat shock results in rescue of 4% of Atg1Δ3D homozygotes to adulthood.

      Atg1Δ3D homozygous larvae expressing Atg1K38Q.Scer\UAS under the control of Scer\GAL4hs.PH in the absence of heat shock show no detectable autophagy when starved, indicating that starvation-induced autophagy is not rescued.

      (Scott et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Atg1K38Q.Scer\UAS
      Atg1K38Q.UAS
      Atg1K38Q
      (Braden and Neufeld, 2016, Liu and Lu, 2010)
      atg1KQ38A
      (Tarayrah-Ibraheim et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (24)