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General Information
Symbol
Dmel\Atg1K38Q.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0216675
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Atg1K38Q, Atg1K38Q
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a dominant-negative form of Atg1.

Amino acid replacement: K38Q.

UASt regulatory sequences drive expression of a mutated form of Atg1.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN has no effect on neuroblast size or number in late pupal stages.

Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Sgs3.PD blocks the formation of the large acidic structures which normally occurs in salivary gland cells approximately 1.5 hours after head eversion, but caspase activation at 2 hours after head eversion is not blocked. This does not prevent breakdown of the salivary gland tissue, as no persistent salivary gland phenotype is seen at 24 hours after puparium formation.

Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Lsp2.PH results in 25% pupal lethality.

At 24 hours after puparium formation, animals expressing Atg1K38Q.Scer\UAS under the control of Scer\GAL4fkh.PH still contain vacuolated salivary gland fragments, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.

The partial lethality caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4GMR.PF can be partially suppressed by co-expression of Atg1K38Q.Scer\UAS. The reduced eye size caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is rescued by co-expression of Atg1K38Q.Scer\UAS.

Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Cg.PA does not result in increased autophagy in the fat body of normally fed animals.

Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Act5C.PP in clones in the wing disc does not result in cell elimination or DNA fragmentation.

Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4Act5C.PP in clones or under the control of Scer\GAL4Cg.PA is sufficient to inhibit starvation-induced autophagy.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The robust formation of Atg8a-positive (or LysoTracker positive) autophagosome vesicles the AdukScer\UAS.cBa-expressing (controlled by Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) fat body clones in fed larvae is not observed when the clones co-express Atg1K38Q.Scer\UAS.

Simultaneous overexpression of Atg1K38Q.Scer\UAS and NcΔN.C-A.Scer\UAS in the remodelling fat body, under the control of Scer\GAL4Lsp2.PH dramatically reduces autophagy and cell death but increases lethality by approximately 40%.

Simultaneous overexpression of Atg1K38Q.Scer\UAS and NcΔN.C-A.Scer\UAS in the remodelling fat body, under the control of Scer\GAL4Lsp2.PH delays pupal development by approximately 4 hours.

Neuroblasts are detected in the mushroom body of 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) and Atg1K38Q.Scer\UAS under the control of Scer\GAL4wor.PA (mushroom body neuroblasts are not seen in wild-type adults).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Expression of Atg1K38Q.Scer\UAS under the control of Scer\GAL4hs.PH in the absence of heat shock results in rescue of 4% of Atg1Δ3D homozygotes to adulthood.

Atg1Δ3D homozygous larvae expressing Atg1K38Q.Scer\UAS under the control of Scer\GAL4hs.PH in the absence of heat shock show no detectable autophagy when starved, indicating that starvation-induced autophagy is not rescued.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Atg1K38Q.Scer\UAS
Atg1K38Q.UAS
Name Synonyms
Secondary FlyBase IDs
    References (15)