Allele Dmel\rumi^Δ26

General Information
Symbol	Dmel\rumi^Δ26	Species	D. melanogaster
Name		FlyBase ID	FBal0216685
Feature type	allele	Associated gene	Dmel\rumi

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms mechanosensory chaeta somatic clone wing margin wing vein
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Acar et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EPgy2}Usp12-46^EY00249 (Acar et al., 2008)
Nature of the lesion	Statement Reference Imprecise excision of the P{EPgy2}CG7023^EY00249 insertion, resulting in a deletion that removes most of the rumi open reading frame. (Acar et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	eclosion defective (Leonardi et al., 2011) lethal \| heat sensitive (Leonardi et al., 2011) neuroanatomy defective (Acar et al., 2008) partially lethal - majority die \| pharate adult stage (Leonardi et al., 2011) short lived (Leonardi et al., 2011) visible \| recessive (Leonardi et al., 2011)
Phenotype Manifest In
	embryonic/larval neuron \| supernumerary (Acar et al., 2008) joint (Leonardi et al., 2011) leg (Leonardi et al., 2011) mechanosensory chaeta \| somatic clone \| heat sensitive (Takeuchi et al., 2011) notal microchaeta (Leonardi et al., 2011) wing margin \| somatic clone \| heat sensitive (Takeuchi et al., 2011) wing vein \| somatic clone \| heat sensitive (Takeuchi et al., 2011)
Detailed Description
	Statement Reference At 25 [o]C, some homozygous rumi[Δ26] mutant animals reach adulthood but display very severe loss of bristles on the thorax and shortening of the legs. These flies die on the food after eclosion or trapped in the pupal case during eclosion, presumably owing to leg defects. As the temperature is decreased to room temperature (21-23 [o]C), approximately three quarter of the rumi[Δ26] mutants exhibit an intermediate loss of microchaetae. At room temperature, the legs are not short, but subtle leg joint defects are commonly observed. When raised at 18 [o]C, most homozygous rumi[Δ26] animals show loss of small patches of microchaetae in anterior parts of the notum and slight increase in the density of microchaetae. No shortening of the legs is observed at 18 [o]C, although most animals show minor defects in leg joints. Homozygous rumi[Δ26] mutant animals fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C. (Leonardi et al., 2011) rumi[Δ26] mitotic clones raised at 30[o]C exhibit the loss of thoracic sensory bristles and margin loss and vein expansion in the wing. (Takeuchi et al., 2011) rumi^Δ26 embryos lacking both maternal and zygotic rumi function raised at 28^oC have a neurogenic phenotype. (Acar et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-4.5 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-10-15 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^{gt-10.12.14.15} (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-10.13-15 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-12.14.15 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-12 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-16-20 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-16-35 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, suppressible by N^gt-24-35 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible \| partially by N^gt-4-35 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible \| partially by N^gt-10-15 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible \| partially by N^gt-10-20 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible \| partially by N^gt-10-35 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible \| partially by N^{gt-10.12.14.15} (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible by N^gt-4.5 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible by N^gt-10.13-15 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible by N^gt-12.14.15 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible by N^gt-12 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible by N^gt-16-20 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible by N^gt-16-35 (Leonardi et al., 2011) rumi^Δ26 has visible \| recessive phenotype, suppressible by N^gt-24-35 (Leonardi et al., 2011)
NOT suppressed by
Statement Reference rumi^Δ26 has lethal \| heat sensitive phenotype, non-suppressible by N^gt-4-35 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, non-suppressible by N^gt-10-20 (Leonardi et al., 2011) rumi^Δ26 has lethal \| heat sensitive phenotype, non-suppressible by N^gt-10-35 (Leonardi et al., 2011)
Suppressor of
Statement Reference rumi^Δ26 is a suppressor of size defective phenotype of N^{Scer\UAS.ΔLNR.T:Ecol\lexA}, Scer\GAL4^Act.PU (Leonardi et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference rumi^Δ26 has joint phenotype, suppressible \| partially by N^gt-4-35 (Leonardi et al., 2011) rumi^Δ26 has leg phenotype, suppressible \| partially by N^gt-4-35 (Leonardi et al., 2011) rumi^Δ26 has mechanosensory chaeta \| somatic clone \| heat sensitive phenotype, suppressible by Hsap\POGLUT1^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^αTub84B.PL (Takeuchi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible \| partially by N^gt-4-35 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible \| partially by N^gt-10-15 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible \| partially by N^gt-10-20 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible \| partially by N^gt-10-35 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible \| partially by N^{gt-10.12.14.15} (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-4.5 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-10.13-15 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-12.14.15 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-12 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-16-20 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-16-35 (Leonardi et al., 2011) rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-24-35 (Leonardi et al., 2011) rumi^Δ26 has wing margin \| somatic clone \| heat sensitive phenotype, suppressible by Hsap\POGLUT1^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^c684 (Takeuchi et al., 2011) rumi^Δ26 has wing vein \| somatic clone \| heat sensitive phenotype, suppressible by Hsap\POGLUT1^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^c684 (Takeuchi et al., 2011)
NOT suppressed by
Statement Reference rumi^Δ26 has mechanosensory chaeta \| somatic clone \| heat sensitive phenotype, non-suppressible by Hsap\KDELC1^{Scer\UAS.T:Zzzz\FLAG} (Takeuchi et al., 2011) rumi^Δ26 has mechanosensory chaeta \| somatic clone \| heat sensitive phenotype, non-suppressible by Scer\GAL4^αTub84B.PL/Hsap\KDELC1^{Scer\UAS.T:Zzzz\FLAG} (Takeuchi et al., 2011) rumi^Δ26 has wing margin \| somatic clone \| heat sensitive phenotype, non-suppressible by Hsap\KDELC2^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^c684 (Takeuchi et al., 2011) rumi^Δ26 has wing margin \| somatic clone \| heat sensitive phenotype, non-suppressible by Scer\GAL4^c684/Hsap\KDELC1^{Scer\UAS.T:Zzzz\FLAG} (Takeuchi et al., 2011) rumi^Δ26 has wing vein \| somatic clone \| heat sensitive phenotype, non-suppressible by Hsap\KDELC2^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^c684 (Takeuchi et al., 2011) rumi^Δ26 has wing vein \| somatic clone \| heat sensitive phenotype, non-suppressible by Scer\GAL4^c684/Hsap\KDELC1^{Scer\UAS.T:Zzzz\FLAG} (Takeuchi et al., 2011)
Suppressor of
Statement Reference rumi^Δ26 is a suppressor of wing disc \| somatic clone \| third instar larval stage phenotype of N^{Scer\UAS.ΔLNR.T:Ecol\lexA}, Scer\GAL4^Act.PU (Leonardi et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Homozygous rumi[Δ26] mutant animals also carrying N[gt-10-20] fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C. Homozygous rumi[Δ26] mutant animals also carrying N[gt-10-35] fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C. Homozygous rumi[Δ26] mutant animals also carrying N[gt-4-35] fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C. Mutants cultured at room temperature (21-23 [o]C), similarly to rumi[Δ26] single mutants, commonly exhibit mild defects in leg joints. At 18 [o]C, the mutants show a mild loss of microchaetae and normal legs. rumi[Δ26] fully suppresses the N[Scer\UAS.ΔLNR.T:Ecol\lexA] wing imaginal disc phenotypes. (Leonardi et al., 2011)
Xenogenetic Interactions
Statement Reference Overexpression of Hsap\POGLUT1[Scer\UAS.T:Zzzz\FLAG] suppresses the sensory organ and wing phenotypes of rumi[Δ26] mitotic clones raised at 30[o]C. Overexpression of Hsap\KDELC1[Scer\UAS.T:Zzzz\FLAG] or Hsap\KDELC2[Scer\UAS.T:Zzzz\FLAG] fails to suppress the sensory organ and wing phenotypes of rumi[Δ26] mitotic clones raised at 30[o]C. (Takeuchi et al., 2011)
Complementation & Rescue Data
Rescued by	rumi^Δ26 is rescued by rumi^Scer\UAS.cAa/Scer\GAL4^unspecified (Acar et al., 2008)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	rumi^Δ26 (Acar et al., 2008, Takeuchi et al., 2011, Leonardi et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Leonardi et al., 2011, Development 138(16): 3569--3578 Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling. [FBrf0214548] Takeuchi et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(40): 16600--16605 Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase. [FBrf0216311] Acar et al., 2008, Cell 132(2): 247--258 Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling. [FBrf0204793]

Allele Dmel\rumiΔ26

Allele Dmel\rumi^Δ26