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General Information
Symbol
Dmel\grpfs1
Species
D. melanogaster
Name
FlyBase ID
FBal0216722
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    The grp coding region is expressed from both GMR and Scer\UAS regulatory sequences.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Homozygous larvae are sensitive to low levels of hydroxyurea in a survival assay.

    grpfs1 has no effect on the apoptotic response seen in eye discs containing dicentric chromosomes (generated using Scer\FLP1hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). This effect is seen at 12-14 hours after heat shock of DcYy+.

    Embryos derived from homozygous females fail to undergo cellularisation and nuclei are either lost from the surface or fuse to form aggregates. The mutant embryos exit mitosis 13 and immediately continue through additional cleavage cycles (in wild-type embryos, the cell cycle pauses after mitosis 13 and cellularisation occurs).

    The mutant embryos do not undergo gastrulation.

    The wing discs of grpfs1 mutant third instar larvae exhibit similar levels of apoptosis to controls in response to X-ray induced irradiation. As in controls, very little apoptosis is seen in the absence of irradiation. grpfs1 mutant larvae exhibit a reduction in irradiation induced cell cycle arrest.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Statement
    Reference

    grpfs1, lokp6 has cell death defective | conditional phenotype, suppressible by grpGUS.cBa, grpfs1

    NOT suppressed by
    Statement
    Reference
    Enhancer of
    Statement
    Reference

    grpfs1 is an enhancer of cell death defective | conditional phenotype of lokp6/lokp6

    Suppressor of
    Statement
    Reference

    grpGUS.cBa, grpfs1 is a suppressor of cell death defective | conditional phenotype of grpfs1, lokp6

    Phenotype Manifest In
    Enhancer of
    Statement
    Reference

    grpfs1 is an enhancer of eye disc | conditional phenotype of lokp6/lokp6

    grpfs1 is an enhancer of wing disc | conditional phenotype of lokp6/lokp6

    grpfs1 is an enhancer of larval neuroblast | larval stage | conditional phenotype of lokp6/lokp6

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The developmental delay and arrest at cycle 3 characteristic for embryos from ddbtZ4344 fathers is not affected in embryos from ddbtZ4344 fathers and grpfs1 mothers.

    grpfs1 partially suppresses the residual apoptotic response seen in lokp6 mutants containing dicentric chromosomes (generated using Scer\FLP1hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). Eye disc apoptosis is abolished 12-14 hours after heat shock of DcYy+, P{FRT(whs)}(8F)105 or P{inv.FRT}Dc3-FrTr1D. Wing disc apoptosis is also suppressed in grpfs1 lokp6 mutants at 12-24 hours after heat shock of DcYy+, P{FRT(whs)}(8F)105 or P{inv.FRT}Dc3-FrTr1D. At 24 hours after heat shock wing disc apoptosis is suppressed in double mutant cells containing Y chromosome dicentrics, but apoptosis is still seen when dicentric chromosomes are generated on the X or autosome.

    Expression of grpGUS.cBa partially suppresses the apoptosis seen in lokp6 grpfs1 double mutant cells containing dicentric chromosomes (generated using Scer\FLP1hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). This effect is seen at 12-14 hours after heat shock of DcYy+.

    A significant number of embryos derived from grpfs1 lokp6 double mutant females have a uniform monolayer of cortical nuclei surrounded by a hexagonal actin network, indicating that they have initiated or completed cellularisation. The nuclei in the double mutant embryos are larger than in wild-type controls. The double mutant embryos consistently proceed through a fifth cortical mitotic cycle (14th cycle) before cellularisation (in wild-type embryos, cellularisation occurs after the 13th cycle). The double mutant embryos also undergo gastrulation. The cephalic furrow does not form in the double mutant embryos.

    grpfs1 lokp6 double mutants exhibit normal telomere protection and chromosome break repair.

    lokp6 enhances the partial loss of cell cycle arrest seen in grpfs1 mutant third instar larval wing discs in response to X-ray induced irradiation. No arrest is seen in the double mutant larvae.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (6)