Allele Dmel\Spn42Da^4.1.Scer\UAS

General Information
Symbol	Dmel\Spn42Da^4.1.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0216764
Feature type	allele	Associated gene	Dmel\Spn42Da

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Osterwalder et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a 2.2kb Spn4.1 isoform fragment from the EST clone LD1292 fused to a 1.1kb fragment from the EST clone GH08104. (Osterwalder et al., 2004)
Carried in construct	P{UASp-Spn4.1} (Osterwalder et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage, with Scer\GAL4^dimm-929 (Osterwalder et al., 2004) lethal \| larval stage, with Scer\GAL4^how-24B (Osterwalder et al., 2004) lethal \| pupal stage, with Scer\GAL4^dimm-929 (Osterwalder et al., 2004)
Phenotype Manifest In
	cephalopharyngeal skeleton, with Scer\GAL4^dimm-929 (Osterwalder et al., 2004) cephalopharyngeal skeleton, with Scer\GAL4^how-24B (Osterwalder et al., 2004)
Detailed Description
	Statement Reference Expression of Spn4^4.1.Scer\UAS under the control of Scer\GAL4^dimm-929 (which drives expression in ~200 peptidergic CNS neurons and in the peritracheal Inka cells) results in a significant increase in both larval and pupal lethality. There are no apparent defects in either embryonic development or hatching. The first instar larvae exhibit no significant defects in either locomotion or feeding behavior; however, there is a block of developmental progress at a characteristic point in larval ecdysis for many Spn4^4.1.Scer\UAS inserts that results in larval death (the strongest effects are found with P{UASp-Spn4.1}283). Some Spn4^4.1.Scer\UAS larvae survive to pupation, wherein they are observed as partially collapsed within their pupal cases. Lethality and molting defects are observed when Spn4^4.1.Scer\UAS is expressed using Scer\GAL4^how-24B to drive expression in muscles and other mesodermally derived tissues, nerves, and trachea, as well as a subset of cells in the CNS (with the larvae arresting development at the L1 to L2 ecdysis). Larvae overexpressing Spn4^4.1.Scer\UAS under the control of Scer\GAL4^how-24B or Scer\GAL4^dimm-929 are able to proceed to the sclerotised double mouthhooks and double ventral plates stage normally but then remain trapped there for up to 24 hours (compared to the whole period lasting for about 1 hour in wild-type). (Osterwalder et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Spn4^4.1.Scer\UAS Spn42Da^4.1.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Osterwalder et al., 2004, J. Neurosci. 24(24): 5482--5491 Drosophila serpin 4 functions as a neuroserpin-like inhibitor of subtilisin-like proprotein convertases. [FBrf0179368]

Allele Dmel\Spn42Da4.1.Scer\UAS

Allele Dmel\Spn42Da^4.1.Scer\UAS