Allele Dmel\MTF-1^Tub.PY

General Information
Symbol	Dmel\MTF-1^Tub.PY	Species	D. melanogaster
Name	Tubulin promoter construct of Yepiskoposyan	FlyBase ID	FBal0216890
Feature type	allele	Associated gene	Dmel\MTF-1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms chemical conditional starvation stress response defective chemical resistant chemical sensitive short lived
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Yepiskoposyan et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A Tubulin promoter drives expression of MTF-1. (Yepiskoposyan et al., 2006)
Carried in construct	P{Tub-MTF-1.Y} (Yepiskoposyan et al., 2006, Vardanyan et al., 2008, Bahadorani et al., 2010, Bahadorani et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant (Bahadorani et al., 2010) chemical sensitive (Bahadorani et al., 2010) short lived \| chemical conditional (Bahadorani et al., 2010) starvation stress response defective (Bahadorani et al., 2010)
Phenotype Manifest In

Detailed Description
	Statement Reference Ubiquitous expression of MTF-1[Tub.PY] sensitises flies to zinc toxicity as compared with wild-type flies. Males expressing MTF-1[Tub.PY] fed with zinc metal die at faster rate in comparison with wild-type flies but show no sensitivity or resistance on cadmium-supplemented medium relative to wild-type. MTF-1[Tub.PY] overexpression sensitises larvae to zinc overload but increases larval resistance to copper and ammonium tetrathiomolybdate (TTM) toxicity. Ubiquitous overexpression of MTF-1[Tub.PY] sensitises flies to starvation induced mortality. (Bahadorani et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference MTF-1^Tub.PY is a suppressor of chemical sensitive phenotype of Dpy-30L1^Scer\UAS.cVa, Scer\GAL4^Act.PU (Vardanyan et al., 2008) MTF-1^Tub.PY is a suppressor of partially lethal - majority die \| pharate adult stage P12 phenotype of ATP7^{dsRNA.Sym.Scer\UAS}, Scer\GAL4^NP2020 (Bahadorani et al., 2010)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Overexpression of MTF-1[Tub.PY] in Scer\GAL4[NP2020]>ATP7[dsRNA.Sym.Scer\UAS] flies significantly enhances pupal survival. (Bahadorani et al., 2010) The increased sensitivity of flies expressing Dpy-30L1[Scer\UAS.cVa] under the control of Scer\GAL4[Act] to cadmium or zinc in the food medium is rescued by co-expression of MTF-1[Tub.PY]. (Vardanyan et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	MTF-1^Tub.PY
Name Synonym	Tubulin promoter construct of Yepiskoposyan
Secondary FlyBase IDs

References ( 4 )
Research paper	Bahadorani et al., 2010, Dis. Model Mech. 3(1-2): 84--91 A Drosophila model of Menkes disease reveals a role for DmATP7 in copper absorption and neurodevelopment. [FBrf0209723] Bahadorani et al., 2010, Neurobiol. Aging 31(7): 1215--1226 Overexpression of metal-responsive transcription factor (MTF-1) in Drosophila melanogaster ameliorates life-span reductions associated with oxidative stress and metal toxicity. [FBrf0215638] Vardanyan et al., 2008, BMC Dev. Biol. 8: 68 Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in Drosophila. [FBrf0207335] Yepiskoposyan et al., 2006, Nucleic Acids Res. 34(17): 4866--4877 Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc. [FBrf0193346]

Allele Dmel\MTF-1Tub.PY

Allele Dmel\MTF-1^Tub.PY