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General Information
Symbol
Dmel\glob1KG06649
Species
D. melanogaster
Name
FlyBase ID
FBal0217290
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P-glob1
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    P{SUPor-P}glob1KG06649
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    glob1KG06649 homozygous and glob1KG06649/Df(3R)Exel8162 transheterozygous females show severe decrease in fertility and some are completely sterile.

    Ovaries from 3-day-old glob1KG06649 homozygous females are smaller compared to age-matched controls largely due to decreased number of late-stage follicles, but no obvious defects are observed in the morphology of other reproductive organs. Most of the eggs laid by these females are poorly developed and fragile with shrunken and flaccid dorsal appendages. Up to stage 7 the mutant egg chambers have morphology comparable to wild-type but during the subsequent stages most of the egg chambers attain irregular arrangement of follicle cells with multilayering, especially at the posterior surface (also observed in glob1KG06649/Df(3R)Exel8162 follicles). In some cases, the nurse cell nuclei form clusters and abnormal positioning of oocytes is also occasionally observed. In about 30% of stage 10 mutant egg chambers the oocyte nucleus is mispositioned, the polar cells are often misplaced too, moreover the 'dumpless' phenotype is also frequent among the mutant follicles. glob1KG06649 mutant egg chambers also display highly increased apoptotic signal in follicle and nurse cells (TUNEL assay) relative to stage-matched wild-type controls.

    Vast majority of glob1KG06649/glob1KG06649 follicles show aberrant organization of F-actin with occasional aggregations from stages 6-7 onwards. During stages 10-12 an F-actin 'cage' develops around nurse cell nuclei to restrain its position and this is almost completely missing in ~90% of the mutant egg chambers; it appears that in some cases this leads to nurse cell nuclei actually entering the oocyte along with the cytoplasmic material pumped into it by nurse cells at this stage. glob1KG06649 follicles also display abnormalities in the distribution of cell adhesion and cell-cell septate junction markers and severe defects in the positioning and morphology of ring canals, in some cases the ring canals are even pushed into the oocyte.

    Similarly, in glob1KG06649 mutant somatic clones in the follicular epithelium, the F-actin is erratically organized, the cells within the clone appear larger and more spherical than the surrounding non-clonal cells and are irregularly arranged.

    Mosaic ovaries containing germline glob1KG06649 clones (created by the DFS/OvoD technique) contain fewer mature eggs and abnormally developing follicles are frequently observed. Females with these mosaic ovaries lay much fewer eggs, about half of them unfertilized (wild-type females lay hardly any unfertilized eggs). Post-stage 7 mosaic egg chambers display disrupted F-actin cytoskeleton in the nurse cells and oocytes, abnormal positioning of the ring canals, and occasionally multiple oocytes within a single follicle (usually two, placed at the opposite ends of the egg chamber).

    glob1KG06649/glob1KG06649 as well as glob1KG06649/Df(3R)Exel8162 mutants display partial lethality with majority of homozygotes dying during embryonic stage, high mortality is observed also in larval stage. Only about a fifth of glob1KG06649 and 4% of glob1KG06649/Df(3R)Exel8162 transheterozygotes survive to adulthood. glob1KG06649 homozygotes as well as glob1KG06649/Df(3R)Exel8162 frequently show melanotic masses, visible especially during larval stages.

    glob1KG06649/glob1KG06649 animals are developmentally delayed and first instar larvae display defects in tracheal liquid clearance as well as the tracheal morphology.

    glob1KG06649/glob1KG06649 adults show age-progressive decline in climbing abilities that is more pronounce than in wild-type controls and a decrease in adult life span accompanied by aging associated impairments.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    Comments
    Comments

    Excision of the insertion can completely revert the mutant phenotype.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    glob1KG06649
    Name Synonyms
    Secondary FlyBase IDs
      References (3)