glob1KG06649 homozygous and glob1KG06649/Df(3R)Exel8162 transheterozygous females show severe decrease in fertility and some are completely sterile.
Ovaries from 3-day-old glob1KG06649 homozygous females are smaller compared to age-matched controls largely due to decreased number of late-stage follicles, but no obvious defects are observed in the morphology of other reproductive organs. Most of the eggs laid by these females are poorly developed and fragile with shrunken and flaccid dorsal appendages. Up to stage 7 the mutant egg chambers have morphology comparable to wild-type but during the subsequent stages most of the egg chambers attain irregular arrangement of follicle cells with multilayering, especially at the posterior surface (also observed in glob1KG06649/Df(3R)Exel8162 follicles). In some cases, the nurse cell nuclei form clusters and abnormal positioning of oocytes is also occasionally observed. In about 30% of stage 10 mutant egg chambers the oocyte nucleus is mispositioned, the polar cells are often misplaced too, moreover the 'dumpless' phenotype is also frequent among the mutant follicles. glob1KG06649 mutant egg chambers also display highly increased apoptotic signal in follicle and nurse cells (TUNEL assay) relative to stage-matched wild-type controls.
Vast majority of glob1KG06649/glob1KG06649 follicles show aberrant organization of F-actin with occasional aggregations from stages 6-7 onwards. During stages 10-12 an F-actin 'cage' develops around nurse cell nuclei to restrain its position and this is almost completely missing in ~90% of the mutant egg chambers; it appears that in some cases this leads to nurse cell nuclei actually entering the oocyte along with the cytoplasmic material pumped into it by nurse cells at this stage. glob1KG06649 follicles also display abnormalities in the distribution of cell adhesion and cell-cell septate junction markers and severe defects in the positioning and morphology of ring canals, in some cases the ring canals are even pushed into the oocyte.
Similarly, in glob1KG06649 mutant somatic clones in the follicular epithelium, the F-actin is erratically organized, the cells within the clone appear larger and more spherical than the surrounding non-clonal cells and are irregularly arranged.
Mosaic ovaries containing germline glob1KG06649 clones (created by the DFS/OvoD technique) contain fewer mature eggs and abnormally developing follicles are frequently observed. Females with these mosaic ovaries lay much fewer eggs, about half of them unfertilized (wild-type females lay hardly any unfertilized eggs). Post-stage 7 mosaic egg chambers display disrupted F-actin cytoskeleton in the nurse cells and oocytes, abnormal positioning of the ring canals, and occasionally multiple oocytes within a single follicle (usually two, placed at the opposite ends of the egg chamber).