FlyBase Allele Report: Dmel\Frq1[UAS.cRPa]

General Information

Symbol

Dmel\Frq1^UAS.cRPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Romero-Pozuelo

FlyBase ID

FBal0217375

Feature type

allele

Associated gene

Dmel\Frq1

Associated Insertion(s)

Carried in Construct

P{UAS-Frq1.RP}

Key Links

Transgenic product class

wild_type

(Romero-Pozuelo et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Romero-Pozuelo et al., 2007)

Mutagen

in vitro construct

(Romero-Pozuelo et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Frq1.RP}

Cytology

Description

UASt regulatory sequences drive expression of a 709bp fragment containing the Frq1 open reading frame.

(Romero-Pozuelo et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Frq1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Dason et al., 2009)

abnormal neuroanatomy, with Scer\GAL4^Toll-6-D42

(Dason et al., 2009)

abnormal neuroanatomy | larval stage, with Scer\GAL4^Toll-6-D42

(Romero-Pozuelo et al., 2007)

abnormal neurophysiology, with Scer\GAL4^elav-C155

(Dason et al., 2009)

abnormal neurophysiology | larval stage, with Scer\GAL4^Toll-6-D42

(Romero-Pozuelo et al., 2007)

Phenotype Manifest In

embryonic/larval neuromuscular junction, with Scer\GAL4^Toll-6-D42

(Romero-Pozuelo et al., 2007)

NMJ bouton, with Scer\GAL4^elav-C155

(Dason et al., 2009)

type I bouton, with Scer\GAL4^Toll-6-D42

(Romero-Pozuelo et al., 2007)

Detailed Description

Statement

Reference

Overexpression of Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^D42 depresses short-term synaptic plasticity in a high Ca[2+] concentration, compared to controls. Expression of Frq1^{Scer\UAS.cRPa} does not change the number of active zones per synapse in 1b boutons nor the number of docked vesicles. These mutants do not show any significant difference in resting Ca[2+] level compared to controls.

Overexpression of Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^elav-C155 significantly reduces the number of 1s synaptic boutons.

(Dason et al., 2009)

Expression of Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^D42 results in a reduction in the total area of the larval neuromuscular junction compared to wild type. The number of type 1s boutons is reduced, while the number of type 1b boutons is not significantly affected.

The amplitude of the evoked excitatory junctional potential (EJP) at the neuromuscular junction is increased compared to wild type in larvae expressing Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^D42. The amplitude and frequency of spontaneous mEJPs is not affected in these flies. Quantal content of type 1b and type 1s boutons is increased in these flies compared to wild type.

(Romero-Pozuelo et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Frq1^UAS.cRPa, Scer\GAL4^elav-C155 has abnormal neurophysiology phenotype, non-enhanceable by Df(3L)7C/fwd³, Scer\GAL4^elav-C155

(Dason et al., 2009)

NOT suppressed by

Statement

Reference

Frq1^UAS.cRPa, Scer\GAL4^elav-C155 has abnormal neurophysiology phenotype, non-suppressible by Df(3L)7C/fwd³, Scer\GAL4^elav-C155

(Dason et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The presence of a fwd³/Df(3L)7C background does not affect the enhanced quantal release found upon overexpression of Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^elav-C155.

The presence of a fwd³/Df(3L)7C background does not affect the reduced number of synaptic boutons observed upon overexpression of Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^elav-C155.

(Dason et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Toll-6-D42/Frq1^UAS.cRPa rescues Df(1)frq^del1

(Dason et al., 2009)

Scer\GAL4^Toll-6-D42/Frq1^UAS.cRPa rescues Df(1)frq^del3

(Dason et al., 2009)

Scer\GAL4^Toll-6-D42/Frq1^UAS.cRPa rescues Df(1)frq^del1/Df(1)frq^del3

(Dason et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Frq1^{Scer\UAS.cRPa}

Frq1^UAS.cRPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Romero-Pozuelo

Secondary FlyBase IDs

References (2)

Report Sections

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