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General Information
Symbol
Scer\GAL4ppk.PG
Species
S. cerevisiae
Name
pickpocket promoter construct of Grueber
FlyBase ID
FBal0217476
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ppk-GAL4, ppkGal4, Gal4ppk
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A ppk promoter fragment regulates expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4ppk.PG drives expression strongly in class IV dendritic arborization neurons and weakly in class III neurons.

Drives expression strongly in class IV dendritic arborization neurons and weakly in class III neurons.

Scer\GAL4ppk.PG drives expression in larval multidendritic neurons (Type II). All of the Type II neurons identified by Scer\GAL4ppk.PG are also labeled with Scer\GAL4Piezo.1.0.

Scer\GAL4ppk.PG drives expression in all class IV dendritic arborizing neurons which co-express Ncra\QFTrpA1.PP.

Scer\GAL4ppk.PG drives expression in peripheral sensory neurons in the legs, wings, body wall, and reproductive tract. In the reproductive tract it is expressed in 2-3 fruitless expressing neurons on either side of the uterus as well as a small number of neurons surrounding the oviduct. The reproductive tract neurons project fine processes between the muscle and epithelial layers that enter and arborize within the lumen of the uterus while other processes bifurcate close to the soma and innervate the lower regions of the common oviduct, both these aborizations run along the inner surface of the epithelial cell layer. Infrequently, expression is also seen in 1-2 neurons near the base of the antennal nerve and the the lateral horn. Afferents from all of the above neurons contribute to the abdominal nerve trunk, most terminating in the thoracico-abdominal ganglion but some extend further in an anterior direction, and a pair of bilateral fibers emerge from the network in the abdominal ganglion, traverse the entire length of the ventral side of the nerve cord, extend through the cervical connective before terminating in the posterior region of the adult gnathal ganglion.

Scer\GAL4ppk.PG drives expression in the adult peripheral neurons, including 2 neurons on each lateral oviduct and 15 neurons on each side of the uterus, organised in 3 clusters.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

dendrite & dendritic arborising neuron, with abd-AUAS.cGa

dendrite & dendritic arborising neuron, with UbxUAS.cUa

dendrite & dorsal multidendritic neuron ddaC, with ssUAS.A5

dendrite & dorsal multidendritic neuron ddaC | larval stage | conditional - heat sensitive, with Scer\GAL80ts.αTub84B, IKKεUAS.cKa

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with IKKεDN.UAS

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with IKKεdsRNA.UAS

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with IKKεG250D.UAS.Tag:MYC

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with IKKεGD5424

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with kat-60L1GD7435

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with EcRB1-ΔC655.W650A.UAS

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with IKKεdsRNA.UAS

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with IKKεG250D.UAS.Tag:MYC

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with IKKεGD5424

Detailed Description
Statement
Reference

Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4ppk.PG-expressing neurons respectively abolishes and severely decreases larval responses (rolling) to harsh touch (44mN) and noxious heat (39.5[o]C).

Hyperpolarization (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4ppk.PG cells in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; there is no effect on the response to gentle touch and there is a small, but significant, increase in locomotion speed, compared to controls.

Inhibition (using Ctet\tetXTNT-LC.UAS) of Scer\GAL4ppk.PG cells in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament and increases the latency of rolling in response to high temperature (local) stimulation, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 625 nm (red) light) of Scer\GAL4ppk.PG cells in larvae significantly increases rolling responses in the absence of pain stimuli, compared to controls; expression of Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus in Scer\GAL4ppk.PG neurons results in their partial degeneration in the larva.

Inhibition (using shi1.UAS at 31[o]C) of Scer\GAL4ppk.PG cells, or the combined set of Scer\GAL4ppk.PG and Scer\GAL419-12 cells, in larvae significantly reduces the probability of backwards motion in response to a head touch, compared to controls.

Silencing neurons expressing Scer\GAL4ppk.PG results in reduced locomotor turning frequency and thermal nociception defects in third instar larvae.

Inhibition (using shi1.UAS at 30[o]C) of Scer\GAL4ppk.PG cells significantly reduces mating receptivity and increases egg laying in virgin females, to the extent that egg laying occurs at a similar rate to that seen in mated females, compared to controls, with no effect on egg laying in mated females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4ppk.PG, par-1HMS00405 has dendrite | pupal stage phenotype, enhanceable by IKKepsilon[+]/IKKεalice

NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Scer\GAL4ppk.PG, par-1HMS00405 has dendrite | pupal stage phenotype, non-suppressible by futschK68/futsch[+]

Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Scer\GAL4ppk.PG
Name Synonyms
pickpocket promoter construct of Grueber
Secondary FlyBase IDs
    References (94)