|Feature type||allele||Associated gene||Dmel\Mical|
|Map ( GBrowse )|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
pr_change=R113 | Mical-PC; R113 | Mical-PH; R113 | Mical-PG; R113 | Mical-PD; R113 | Mical-PA; R113 | Mical-PB; R113 | Mical-PE; R113 | Mical-PF; R113 | Mical-PI; R113 | Mical-PK; R113 | Mical-PL; R113 | Mical-PM
comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: R113@.
|Phenotype Manifest In|
Projection errors and branching defects are seen in the SNa pathway in MicalK583/Df(3R)swp2MICAL embryos, although the frequency of defects (27.7%) is only slightly increased compared to wild-type embryos (11.2%). The number of boutons in the cleft between muscles 6 and 7 is increased compared to controls in MicalK583/Df(3R)swp2MICAL third instar larvae and the muscle 6+7 surface area is also consistently larger than wild type. The neuromuscular junctions of MicalK583/MicalI666 embryos do not show any obvious structural differences to wild type. MicalK583/MicalI666 first instar larvae have shorter synaptic branches and more clustered boutons at the neuromuscular junction than control larvae. The overall organisation of the presynaptic terminal at the neuromuscular junction of MicalK583/MicalI666 third instar larvae appears ultrastructurally normal. However, there are dramatic changes on the postsynaptic side compared to wild type; the subsynaptic reticulum (SSR) occupies a larger volume around presynaptic boutons and is reduced to small cisternae that are fewer in number and less convoluted than in wild type. The usually highly structured postsynaptic domain is completely disorganised; myofilaments accumulate in postsynaptic regions instead of being strictly separated from the SSR. Myosin-rich thick filaments, often surrounded by associated thin filaments, are seen at the plasma membrane and even directly beneath active zones. The thick filaments are distributed singly or in loose bundles in the cytoplasm and are oriented in all directions. In nearly all cases, boutons are deeply embedded in the muscle tissue and covered with a thick layer of sarcoplasm and disorganised myofilaments. MicalK583/MicalI666 third instar larvae show disruption of the sarcomeric patterning normally seen in the somatic muscles, showing misaligned myofilaments. These misaligned myofilaments are located mainly in the peripheral cortex of the muscle fibre and do not, in most cases, extend to the innermost core. The average length of the disorganised filament bundles appears longer than the length of sarcomeres, and the coat of disorganised myofilaments is often interrupted by vessel-like channels (which are derived from terminal tracheal branches). Synaptic boutons of larvae show strong postsynaptic accumulations of filamentous actin (in contrast to wild type) and F-actin also colocalises with the chaotically arranged myosin filaments in the peripheral regions of the muscle.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
|Secondary FlyBase IDs|
|References ( 3 )|