Allele Dmel\Mical^K583

General Information
Symbol	Dmel\Mical^K583	Species	D. melanogaster
Name		FlyBase ID	FBal0217515
Feature type	allele	Associated gene	Dmel\Mical

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Beuchle et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:5,846,768..5,846,768 na_change=A5846768T pr_change=R113 \| Mical-PC; R113 \| Mical-PH; R113 \| Mical-PG; R113 \| Mical-PD; R113 \| Mical-PA; R113 \| Mical-PB; R113 \| Mical-PE; R113 \| Mical-PF; R113 \| Mical-PI; R113 \| Mical-PK; R113 \| Mical-PL; R113 \| Mical-PM reported_pr_change=R113@ comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. (Beuchle et al., 2007)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: R113@. (Beuchle et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| embryonic stage (with Df(3R)swp2^MICAL) (Beuchle et al., 2007) neuroanatomy defective \| first instar larval stage (with Mical^I666) (Beuchle et al., 2007) neuroanatomy defective \| larval stage (Beuchle et al., 2007) neuroanatomy defective \| third instar larval stage (with Df(3R)swp2^MICAL) (Beuchle et al., 2007) neuroanatomy defective \| third instar larval stage (with Mical^I666) (Beuchle et al., 2007) visible (with Df(3R)swp2^MICAL) (Hung et al., 2010)
Phenotype Manifest In
	abdominal segmental nerve (with Df(3R)swp2^MICAL) (Beuchle et al., 2007) bouton (with Df(3R)swp2^MICAL) (Beuchle et al., 2007) bouton (with Mical^I666) (Beuchle et al., 2007) embryonic/larval muscle system (with Df(3R)swp2^MICAL) (Beuchle et al., 2007) embryonic/larval neuromuscular junction (Beuchle et al., 2007) embryonic/larval neuromuscular junction (with Df(3R)swp2^MICAL) (Beuchle et al., 2007) embryonic/larval neuromuscular junction (with Mical^I666) (Beuchle et al., 2007) embryonic/larval somatic muscle (with Mical^I666) (Beuchle et al., 2007) filamentous actin (with Mical^I666) (Beuchle et al., 2007) macrochaeta (with Df(3R)swp2^MICAL) (Hung et al., 2010) sarcomere (with Mical^I666) (Beuchle et al., 2007)
Detailed Description
	Statement Reference Surviving Mical[K583]/Df(3R)swp2[MICAL] adults usually have defects in bristle morphology. (Hung et al., 2010) Projection errors and branching defects are seen in the SNa pathway in Mical^K583/Df(3R)swp2^MICAL embryos, although the frequency of defects (27.7%) is only slightly increased compared to wild-type embryos (11.2%). The number of boutons in the cleft between muscles 6 and 7 is increased compared to controls in Mical^K583/Df(3R)swp2^MICAL third instar larvae and the muscle 6+7 surface area is also consistently larger than wild type. The neuromuscular junctions of Mical^K583/Mical^I666 embryos do not show any obvious structural differences to wild type. Mical^K583/Mical^I666 first instar larvae have shorter synaptic branches and more clustered boutons at the neuromuscular junction than control larvae. The overall organisation of the presynaptic terminal at the neuromuscular junction of Mical^K583/Mical^I666 third instar larvae appears ultrastructurally normal. However, there are dramatic changes on the postsynaptic side compared to wild type; the subsynaptic reticulum (SSR) occupies a larger volume around presynaptic boutons and is reduced to small cisternae that are fewer in number and less convoluted than in wild type. The usually highly structured postsynaptic domain is completely disorganised; myofilaments accumulate in postsynaptic regions instead of being strictly separated from the SSR. Myosin-rich thick filaments, often surrounded by associated thin filaments, are seen at the plasma membrane and even directly beneath active zones. The thick filaments are distributed singly or in loose bundles in the cytoplasm and are oriented in all directions. In nearly all cases, boutons are deeply embedded in the muscle tissue and covered with a thick layer of sarcoplasm and disorganised myofilaments. Mical^K583/Mical^I666 third instar larvae show disruption of the sarcomeric patterning normally seen in the somatic muscles, showing misaligned myofilaments. These misaligned myofilaments are located mainly in the peripheral cortex of the muscle fibre and do not, in most cases, extend to the innermost core. The average length of the disorganised filament bundles appears longer than the length of sarcomeres, and the coat of disorganised myofilaments is often interrupted by vessel-like channels (which are derived from terminal tracheal branches). Synaptic boutons of larvae show strong postsynaptic accumulations of filamentous actin (in contrast to wild type) and F-actin also colocalises with the chaotically arranged myosin filaments in the peripheral regions of the muscle. (Beuchle et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	K583 (Hung et al., 2010) Mical^K583 (Kirilly et al., 2009) mical^K583 (Beuchle et al., 2007) milch^K583 (Beuchle et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Hung et al., 2010, Nature 463(7282): 823--827 Mical links semaphorins to F-actin disassembly. [FBrf0214846] Kirilly et al., 2009, Nat. Neurosci. 12(12): 1497--1505 A genetic pathway composed of Sox14 and Mical governs severing of dendrites during pruning. [FBrf0209365] Beuchle et al., 2007, Mech. Dev. 124(5): 390--406 Drosophila MICAL regulates myofilament organization and synaptic structure. [FBrf0200539]

Allele Dmel\MicalK583

Allele Dmel\Mical^K583